Evaluation of Stability and Antibacterial Activity of
Various Concentrations of Triple Antibiotic Paste
against Enterococcus faecalis: An in vitro Study
Citation Information :
Evaluation of Stability and Antibacterial Activity of Various Concentrations of Triple Antibiotic Paste against Enterococcus faecalis: An in vitro Study. World J Dent 2017; 8 (5):403-406.
Aim: The aim of this study is to evaluate the stability and antibacterial effect of various concentrations of triple antibiotic paste (TAP) against Enterococcus faecalis with antibiotic
susceptibility testing.
Materials and methods: The stability and antibacterial effect of TAP was tested with antibiotic susceptibility test against E. faecalis by using Agar disk diffusion method over a period
of 1, 3, 7, 14, and 21 days. The samples were divided into three groups: group I (1% TAP), group II (2% TAP), group III (3% TAP), and group IV (chlorhexidine as control). The zones of
inhibition were measured after 24 hours and were recorded in millimeters and the same procedure was repeated again after 3, 7, 14, and 21 days.
Results: Higher concentration of 3% TAP showed superior antibacterial effect against E. faecalis compared with 1% TAP, 2% TAP, and control group (chlorhexidine). The antibacterial
efficacy increased more rapidly after 21 days when compared with 24 hours, 3 days, and 7 days.
Conclusion: Under specified limitations of this in vitro study, higher concentration of TAP can be used as an effective intracanal medicament against E. faecalis. It is clearly experimented
that longer duration of application of intracanal medicament increases its antibacterial effect against E. faecalis. Clinical significance: Triple antibiotic paste can be prepared, stabilized, and used for a period of 21 days.
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