GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study
SM Apoorva, Divya Bhat, Akanksha Garg, A Suchetha, N Sapna, Lakshmi Puzhankara
Citation Information :
Apoorva S, Bhat D, Garg A, Suchetha A, Sapna N, Puzhankara L. GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study. World J Dent 2014; 5 (1):42-46.
The literature suggests that periodontal disease and diabetes mellitus share a two-way relationship. The aim of this study was to evaluate and compare the levels of adrenomedullin (ADM) in gingival crevicular fluid (GCF) of periodontally healthy and periodontitis patients with or without type 2 diabetes with different glycemic controls.
Methods
Ninety patients were included in the study and were divided into five groups based on CPI scores and ADA classification of diabetes. Probing pocket depth (PPD) and clinical attachment level (CAL) were measured in all the subjects. GCF was collected from all the participants using micropipettes. ADM levels were measured in GCF samples by enzyme-linked immunosorbent assay.
Results
The results showed higher levels of ADM in patients with periodontitis as compared to healthy group. Significant correlation was present between PPD and CAL and ADM levels in all periodontitis patients with or without type 2 diabetes.
Conclusion
Increase in GCF levels of ADM from periodontal health to disease and in periodontitis patients with type 2 diabetes with the worsening of glycemic control underlines the possible role of ADM in mounting a protective response to worsening disease state.
How to cite this article
Garg A, Suchetha A, Sapna N, Apoorva SM, Bhat D, Puzhankara L. GCF Adrenomedullin Levels in Healthy and Periodontitis Patients with or without Type 2—Diabetes Mellitus: Clinicobiochemical Study. World J Dent 2014;5(1):42-46.
In vitro assessment of antimicrobial peptides as potential agents against several oral bacteria. J Antimicrob Chemother 2006;58:198-201.
Epithelial antimicrobial peptides in host defense against infection. Respir Res 2000;1:141-150.
Antimicrobial peptides in the oral environment: Expression and function in health and disease. Curr Issues Mol Biol 2005;7:119-133.
Radioimmunoassay quantification of adrenomedullin in human gingival crevicular fluid. Arch Oral Biol 2006;51: 334-338.
Expression of adrenomedullin and its receptors in human salivary tissue. J Dent Res 2004;83:333-337.
Adrenomedullin expression in pathogen-challenged oral epithelial cells. Peptides 2001;22:1485-1489.
Adrenomedullin has multiple roles in disease stress: Development and remission of the inflammatory response. Microsc Res Tech 2002;57:120-129.
Periodontitis and diabetes interrelationships: Role of inflammation. Ann Periodontol 2001;6(1):125-137.
The relationship between adrenomedullin, metabolic factors and vascular function in individuals with type 2 diabetes. Diabetes Care 2007 Jun;30(6):1513-1519.
Available at: http://http//:www.whocollab.od.mah.se/expl/orhcpitn97.html (19-07-2009).
Diabetes mellitus and periodontal disease. Periodontology 2000. 2007;44:127-153.
Periodontal disease: the sixth complication of diabetes mellitus. Diabetes Care 1993;16(1):329-334.
Periodontal disease and diabetes mellitus: a two-way relationship. Ann Periodontol 1998;3(1):51-61.
Evaluation of gingival crevicular fluid adrenomedullin and human neutrophil peptide 1-3 levels of patients with different periodontal diseases. J Periodontol 2010;81:284-291.
Adrenomedullin and diabetes mellitus. Diabetes Metab Res Rev 2001;17: 321-329.
Plasma adrenomedullin levels in type 1 diabetes: relationship with clinical parameters. Diabetes Care 1998;21: 999-1003.
Relationship between plasma adrenomedullin levels and metabolic control, risk factors, and diabetic microangiopathy in patients with type 2 diabetes. Diabetes Care 2000;23:864-867.
Diabetes mellitus. In: Rose LF, Genco RJ, Mealy BL, Cohen WD, editors. Periodontal Medicine. Hamilton, Ontario: BC Decker Inc.; 2000. p. 121-151.
Diabetes mellitus. In: Braunwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL, Jameson JL, editors. Harrison's Principles of Internal Medicine. 16th ed., New York: McGraw Hill Companies 2005;2: p 2137-2152.
The effect of improved periodontal health on metabolic control in type 2 diabetes mellitus. J Clin Periodontol 2005;32(3):266-272.
Clinical and metabolic changes after conventional treatment of type 2 diabetic patients with chronic periodontitis. J Periodontol 2006;77(4):591-598.
Screening of periodontitis with salivary enzyme test. Journal of Oral Science 2006;48(4): 177-183.
Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 1998;9(3):248-266.
Antimicrobial peptides of the oral cavity. Periodontology 2000. 2009;51:152-180.
Adrenomedullin suppresses tumour necrosis factor alphainduced CXC chemokine ligand 10 production by human gingival fibroblasts. Clin Exp Immunol 2008;152:568-575.
Adrenomedullin production in fibroblasts: Its possible function as a growth regulator of Swiss 3T3 cells. Endocrinology. 1998;139:2552-2563.
Interaction of adrenomedullin and calcitonin gene-related peptide with the periodontal pathogen porphyromonas gingivalis. FEMS Immunol Med Microbiol 2007;49:91-97.
