World Journal of Dentistry

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VOLUME 1 , ISSUE 3 ( October-December, 2010 ) > List of Articles

RESEARCH ARTICLE

Fibroblast Proliferation due to Exposure to a Platelet Concentrate: An in vitro Study

Shelly Ahuja

Citation Information : Ahuja S. Fibroblast Proliferation due to Exposure to a Platelet Concentrate: An in vitro Study. World J Dent 2010; 1 (3):163-166.

DOI: 10.5005/jp-journals-10015-1033

Published Online: 00-12-2010

Copyright Statement:  Copyright © 2010; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Introduction

The major cellular events in the tissue repair are mitogenesis, migration and metabolism. The proteins responsible for coordination of these events are called “growth factors”. The activated platelets at the wound margins release several growth factors, such as PDGF, TGF-β and EGF, etc., and plasma exudates also provide an important source of TGF-β factors.

Materials and methods

Periodontal ligament fibroblast obtained from third molar impaction surgery, periodontal ligaments were cultured under standard conditions and spread on 96 well tissue culture plates. Platelet concentrate was obtained after centrifugation of 350-400 ml of blood at 1000 and 5000 rpm. 15 μl of platelet concentrate was added to each well. The proliferation rate of test and control group was determined by Redox indicator (Alamar blue® assay). The number of cells were counted by neu bar counting chamber after 24, 48 and 72 hours.

Results

The proliferation activity of cells was considerably higher in the platelet concentrate group (test group) than the control group. The difference was highly significant upto 72 hours after addition of platelet concentrates (Mann-Whitney U test p < 0.001).

Conclusion

A cellular effect of the platelet concentrate is clearly discernible. It was concluded that the use of platelet concentrate is an effective modality of regeneration.


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