Aim: To compare and assess the efficacy of buccal pad fat in combination with demineralized freeze-dried bone allograft (DFDBA) as a barrier material vs buccal pad fat alone in the treatment of grade II furcation defects. Materials and methods: Participants (n = 12) in the study age-group of 30–50 years old with no systemic disease, chronic generalized periodontitis, and grade II furcation defects were randomly assigned to groups I and II. Group I patients were assigned DFDBA and buccal fat pad (BFP), while group II received BFP alone. At baseline, 3 months, and 6 months, clinical measures such as gingival index (GI), plaque index (PI), relative clinical attachment level (CAL), horizontal pocket depth, vertical pocket depth, probing pocket depth (PPD), and radiographic findings were compared between groups I (buccal pad and DFDBA) and II (buccal pad). Results: In both groups, clinical parameters from baseline to 6 months at varied time intervals revealed a significant reduction in mean GI and PI. Intergroup comparisons, however, showed a clinically and statistically significant reduction in group I at the end of 6 months. Conclusion: According to the findings of this study, the association between DFDBA and BFP is a practical and acceptable therapeutic method that leads to higher patient compliance and has high regenerative potential. Clinical significance: In the treatment of grade II furcation defects, the combined treatment of BFP and DFDBA produced a good response.