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VOLUME 15 , ISSUE 1 ( January, 2024 ) > List of Articles


Evaluation of Cytotoxicity of Green Synthesized Strontium Fluorapatite Nanoparticles on Human Gingival Fibroblasts: An In Vitro Study

Nisshitha Rao Setvaji, Aravind Kumar Subramanian

Keywords : Cytotoxicity test, Fluorapatite, Human gingival fibroblasts, MTT assay, Nanoparticle, Strontium

Citation Information : Setvaji NR, Subramanian AK. Evaluation of Cytotoxicity of Green Synthesized Strontium Fluorapatite Nanoparticles on Human Gingival Fibroblasts: An In Vitro Study. World J Dent 2024; 15 (1):25-29.

DOI: 10.5005/jp-journals-10015-2357

License: CC BY-NC 4.0

Published Online: 20-02-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: To evaluate the cytotoxicity of the green synthesized strontium fluorapatite nanoparticles (SrFAp NPs) on human gingival fibroblast (HGF) cell lines using MTT assay. Materials and methods: Strontium fluorapatite nanoparticles (SrFAP NPs) used in the present study were developed using green synthesis method from plant extracts of Equisetum arvense (Horsetail) and Laminariales (Kelp) along with fluoride and hydroxyapatite precursors. The HGF cell lines were treated with different concentrations of 10, 20, 30, 40, and 80 μg/mL of SrFAp NPs and cultured. The cultured media was treated with MTT reagent and incubated. The number of damaged or dying cells was represented by the absorbance of the produced formazan at 570 nm and measured using a UV Vis spectrophotometer. Results: The cell viability decreased with increasing concentrations of SrFAp NPs. At the lowest concentrations of 10 μg/mL, cell viability was 83%, and at 20, 30, 40, and 80 μg/mL, the cell viability was 81, 79, 74, and 70%, respectively. One-way analysis of variance (ANOVA) showed a significant difference between the different concentrations (p = 0.000, F = 14.682). Conclusion: Green synthesized SrFAp NPs showed good biocompatibility against HGF using the MTT assay. At the lowest concentration of 10 μg/mL, cell viability was 83% and at the highest concentration of 80 μg/mL NPs, the cell viability was 70%. Clinical significance: Strontium fluorapatite nanoparticles (SrFAp NPs) were developed by green synthesis method, which is nontoxic and environmentally friendly. The NPs were hypothesized to have enamel remineralizing properties which could be further applied clinically to prevent white spot lesions.

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