Citation Information :
Reshma AP, Varghese SS, Pampadykandathil LP. Comparative Evaluation of Platelet-derived Growth Factor Isoforms between Platelet-rich Fibrin Prepared with Three Different Centrifugation Protocols. World J Dent 2023; 14 (11):977-982.
Aim: The aim of the present study is to evaluate the impact of centrifugation protocols and release profile of platelet-derived growth factor (PDGF) isoforms among platelet-rich fibrin (PRF) variants that were prepared using different relative centrifugation forces (RCF) and centrifugation times.
Materials and methods: The PDGF isoforms (PDGF-AA, PDGF-BB, and PDGF-AB) release was evaluated using enzyme-linked immunosorbent assay (ELISA) (Quantikine ELISA kits (R&D Systems, Inc, Minneapolis, Minnesota, United States of America).
Results: The highest reported PDGF isoforms released from platelet concentrates (PCs) was PDGF-BB, followed by PDGF-AB and PDGF-AA. I-PRF released significantly higher growth factors (GF) when compared to A-PRF (p < 0.001) and L-PRF (p < 0.005).
Conclusion: The current study indicated that a systematic reduction in the applied RCF increased GF levels. Thus, the determined GFs PDGF-AA, -AB, and -BB were the lowest in L-PRF, which was prepared within the high RCF range. However, reductions in RCF significantly increased PDGF isoforms in I-PRF than in A-PRF and L-PRF.
Clinical significance: The differences in the centrifugation protocol have an influence on the release profile of PDGF isoforms among PRF variants. I-PRF prepared with low RCF range can be recommended for fast delivery of PDGF isoforms and may prove clinically beneficial for future regenerative procedures.
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