World Journal of Dentistry

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VOLUME 14 , ISSUE 11 ( November, 2023 ) > List of Articles

ORIGINAL RESEARCH

Comparative Evaluation of Platelet-derived Growth Factor Isoforms between Platelet-rich Fibrin Prepared with Three Different Centrifugation Protocols

Arunima Padmakumar Reshma, Sheeja S Varghese, Lizymol P Pampadykandathil

Keywords : Centrifugation, Growth factors, Platelet-derived growth factor, Platelet-rich fibrin, Relative centrifugal force

Citation Information : Reshma AP, Varghese SS, Pampadykandathil LP. Comparative Evaluation of Platelet-derived Growth Factor Isoforms between Platelet-rich Fibrin Prepared with Three Different Centrifugation Protocols. World J Dent 2023; 14 (11):977-982.

DOI: 10.5005/jp-journals-10015-2327

License: CC BY-NC 4.0

Published Online: 16-01-2024

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

Aim: The aim of the present study is to evaluate the impact of centrifugation protocols and release profile of platelet-derived growth factor (PDGF) isoforms among platelet-rich fibrin (PRF) variants that were prepared using different relative centrifugation forces (RCF) and centrifugation times. Materials and methods: The PDGF isoforms (PDGF-AA, PDGF-BB, and PDGF-AB) release was evaluated using enzyme-linked immunosorbent assay (ELISA) (Quantikine ELISA kits (R&D Systems, Inc, Minneapolis, Minnesota, United States of America). Results: The highest reported PDGF isoforms released from platelet concentrates (PCs) was PDGF-BB, followed by PDGF-AB and PDGF-AA. I-PRF released significantly higher growth factors (GF) when compared to A-PRF (p < 0.001) and L-PRF (p < 0.005). Conclusion: The current study indicated that a systematic reduction in the applied RCF increased GF levels. Thus, the determined GFs PDGF-AA, -AB, and -BB were the lowest in L-PRF, which was prepared within the high RCF range. However, reductions in RCF significantly increased PDGF isoforms in I-PRF than in A-PRF and L-PRF. Clinical significance: The differences in the centrifugation protocol have an influence on the release profile of PDGF isoforms among PRF variants. I-PRF prepared with low RCF range can be recommended for fast delivery of PDGF isoforms and may prove clinically beneficial for future regenerative procedures.


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