Aim: The present in vitro study aimed to evaluate and compare the remineralization potential of—2% sodium fluoride (NaF), 2% NaF along with Psoralea corylifolia L. (Leguminosae) (Bakuchi), and white mineral trioxide aggregate (MTA) using Vickers hardness number (VHN) and scanning electron microscopy–energy dispersive X-ray analyzer (SEM-EDX). Materials and methods: The present in vitro study was conducted on 45 sound premolars, sectioned into two parts, further embedded in acrylic resins, polished, and divided into three groups of normal, demineralized, and remineralized teeth (n = 15). The remineralized teeth were further subdivided into three groups (n = 15) as remineralized with 2% NaF, 2% NaF, and Psoralea corylifolia L. (Bakuchi) and white MTA. Specimens of each group were treated with the above-mentioned remineralizing agents and then subjected to VHN and SEM-EDX for further evaluation. Results: The test results showed significantly the highest VHN and the emission peak of elements under the EDX test, such as calcium (Ca), phosphorous (P), oxygen, and fluorine (F) with the group remineralized with NaF + Bakuchi (group III-B). Conclusion: Sodium fluoride (NaF) + Bakuchi showed effective results in VHN and SEM-EDX with no antagonist interaction. Thus, Psoralea corylifolia L. has a remineralizing effect on the tooth and can be used as a herbal extract for effective reduction in pathogenic bacteria. Clinical significance: Psoralea corylifolia L. (Bakuchi), when mixed with NaF, has great potential for use in dentifrices and mouthwash and has revealed appreciable results on remineralization of artificially demineralized enamel lesions when compared to the MTA and NaF alone.