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VOLUME 13 , ISSUE S1 ( Supplementary Issue 1, 2022 ) > List of Articles


Evaluation of Antibacterial Effect of Bioactive Primer over Chemical Primers in Bacterial-induced Secondary Caries: An In Vitro Study

Saumya G Nair, Rony T Kondody, Manjusha Nambiar

Keywords : 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer, Chlorhexidine digluconate solution, Fluorescent microscopy, Green tea extract solution

Citation Information : Nair SG, Kondody RT, Nambiar M. Evaluation of Antibacterial Effect of Bioactive Primer over Chemical Primers in Bacterial-induced Secondary Caries: An In Vitro Study. World J Dent 2022; 13 (S1):S45-S50.

DOI: 10.5005/jp-journals-10015-2132

License: CC BY-NC 4.0

Published Online: 01-10-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Aim: The study aimed to evaluate the protective effect of bioactive primer over chemical primers in and around the margins of class V composite restorations. Materials and methods: A total of 30 extracted mandibular premolars were chosen. These specimens were randomly divided into three groups (10 samples in each). At first, class V cavity was prepared, and cavity walls were then pretreated with 4-(2-hydroxyethyl)-1-pperazineethanesulfonic acid (HEPES) buffer solution (group I), chlorhexidine (CHX) digluconate (group II), and extract of an enriched fraction of green tea (group III). The in vitro model with Streptococcus mutans [25175 American Type Culture Collection (ATCC)] was incubated for a period of 4 days. The dentin and enamel margins of the cavity walls were analyzed by fluorescence microscopy analysis. Results: The comparison of the mean distance from restoration in the dentinal region and also in the enamel region revealed that the distance at which the initial lesion was seen was approximately similar in all groups, while group III green tea extract (GTE) showed a zone of inhibition adjacent to restoration, which indicated the protective effect of green tea extract primer. Whereas mean lesion depth in the enamel and dentin region showed that lesion depth was less for group III compared to the other two groups. Therefore overall result demonstrated that the green tea extract primer significantly inhibited the development of secondary caries adjacent to the dentin resin interface at cavity walls. At the same time, no significant inhibition was observed in the enamel margins of cavity walls. Conclusion: Application of a green tea extract primer inhibited secondary caries formation. Neither the application of CHX nor HEPES buffer had much effect on the inhibitory effect on secondary caries. Clinical significance: Within the limitation of this study, green tea extract has shown the potential to be used in preventing the development of secondary caries. While current chemical primers were less effective, more potent chemical agents needed to develop as an alternative in the inhibition of secondary caries.

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