Aim: To evaluate and compare the efficacy of the topical application of Boswellia serrata gel and chlorhexidine gluconate gel in depletion of gingival inflammation and plaque in study participants diagnosed with plaque-induced gingivitis. Materials and methods: A total of 60 study participants diagnosed with chronic gingivitis in the age-group 18–40 years signed up for the research and were categorized into three equal groups. Group I: scaling + Boswellia serrata gel, group II: Scaling + Chlorhexidine gluconate gel, group III: scaling + placebo gel. A detailed case history including Gingival Index (Loe and Silness, 1963), Plaque Index (Silness and Loe, 1964), and Sulcus Bleeding Index (Muhlemann & Son, 1971) was recorded in their first visit which were reassessed after 14 days (second visit) and 21 days (third visit) and comparison was made with the baseline data. Results: The mean scores for Plaque Index, Gingival Index, and Sulcus Bleeding Index were significantly decreased during 2nd and 3rd visit after 14 and 21 days, respectively when compared with baseline scores. No significant difference was recorded between group I and group II (test groups) whereas there was significant difference between the test groups and group III (control group). Conclusion: Both Boswellia serrata gel and chlorhexidine gluconate gel were similar in action when used for topical application along with scaling and polishing in the management of chronic gingivitis study participants. Clinical significance: Boswellia serrata gel can be used as an adjunct to scaling and root planing as a long-term intervention due to its low cost availability and better tolerance when compared to the widespread side effects of chlorhexidine.