Periodontal In Vitro Cells Response on Zirconia Implant Surfaces Textured with Milled Machining Micropores
Mariana B da Cruz, Joana F Marques, Neusa Silva, Sara Madeira, Óscar Carvalho, Filipe S Silva, João MM Caramês, António DSP da Mata
Conventional milling, Human fetal osteoblasts, Human gingival fibroblast, Micro-pore, Zirconia
Citation Information :
da Cruz MB, Marques JF, Silva N, Madeira S, Carvalho Ó, Silva FS, Caramês JM, da Mata AD. Periodontal In Vitro Cells Response on Zirconia Implant Surfaces Textured with Milled Machining Micropores. World J Dent 2022; 13 (4):307-315.
Aim: The aim of this in vitro study was to investigate the influence of milled micropores created on zirconia implant surfaces with different widths, depths, and spacing on osteoblasts and fibroblasts cells response.
Materials and methods: A total of 108 zirconia disks were produced using press-and-sintering techniques and randomly assigned in five groups textured with milled micropores with different dimensions of widths, depths, and spacings. All samples including control samples were sandblasted and acid-etched (SBAE). Fibroblasts and osteoblasts were cultured on disks for up to 14 days. Morphology and cellular adhesion were observed using scanning electron microscopy (SEM). Cell viability and proliferation were assessed using CellTiter-Blue® reagent and the alkaline phosphatase (ALP) activity was evaluated using a fluorometric enzyme assay. The levels of interleukin-1β, collagen type I, interleukin-8, and osteopontin were assessed using an appropriate enzyme-linked immunosorbent assay (ELISA) kit. The data was subject to statistical analysis performed using the IBM® SPSS® 24.0 software for Mac (SPSS, Chicago, USA). Group comparisons were tested using two-way ANOVA or Mann-Whitney U test with Tukey's multiple comparisons (Tukey's post hoc). Results were presented as mean ± standard deviation and the significance level was set at p < 0.05.
Results: Cell viability and proliferation increase over time in all groups, in both cell lines, without significant differences between them. SEM images reveal adherent cells after 1 day of culture. The production of interleukin-1β, collagen type I, interleukin-8, and osteopontin did not show statistically significant differences, as well as the ALP activity when all groups were compared.
Conclusion: Milled micro-pore dimensions between 10 μm and 100 μm on Zirconia implant surfaces with different widths, depths, and spacings did not improve periodontal cells behavior in SBAE surfaces.
Clinical significance: The production of milled micro-pore modified Zirconia implant surfaces may help us to improve their clinical behavior.
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