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VOLUME 13 , ISSUE 1 ( January-February, 2022 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Interleukin 6 Levels in Gingival Crevicular Fluid and Periodontal Ligament on Application of Orthodontic Forces

Akhil Shetty, Mahima Jain, K Sneha, Veena Shetty, Shama Rao, Ashutosh Shetty

Keywords : ELISA, Gingival crevicular fluid, Interleukin 6, Orthodontic force, Periodontal ligament

Citation Information : Shetty A, Jain M, Sneha K, Shetty V, Rao S, Shetty A. Evaluation of Interleukin 6 Levels in Gingival Crevicular Fluid and Periodontal Ligament on Application of Orthodontic Forces. World J Dent 2022; 13 (1):16-20.

DOI: 10.5005/jp-journals-10015-1891

License: CC BY-NC 4.0

Published Online: 29-12-2021

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Aim and objective: The purpose of this study was to assess and compare the interleukin 6 levels in gingival crevicular fluid and periodontal ligament before and after application of the orthodontic force. Materials and methods: It is a split mouth study where the gingival crevicular fluid (GCF) and periodontal ligament (PDL) samples of 20 individuals were collected from control and experimental site. GCF samples were collected using 1–5 μL calibrated volumetric microcapillary pipette. PDL samples were collected from the extracted tooth, and PDL tissue was scraped using Gracey curette and was immediately transferred to Eppendorf microcentrifuge tubes (Sigma–Aldrich; St. Louis, MO, USA). The collected samples were stored at −80°C for further analysis using ELISA kit (Human IL-6 ELISA MAXTM Delux-5 plates) and the optical density was recorded at 450 nm. Result: In the study it was observed that there was an increase in IL-6 concentration in the PDL in the experimental group (3rd day of force application). The concentration of IL-6 was recorded after 3rd day of force application between the experimental groups of PDL and GCF and an increase in the levels of IL-6 was noted. The increase in the level of IL-6 in these groups was statistically insignificant. Conclusion: The study depicted similar concentration level of IL-6 in GCF and PDL on the 3rd day of application of orthodontic force. Therefore, GCF can be used as a biomarker in the assessment of IL-6 for up to 3 days of force application instead of PDL. Clinical significance: GCF can be assessed to know the concentration of cytokines (IL-6) upto 3 days of force application in the PDL, as collection of GCF is a non-invasive technique.


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  1. Başaran G, Özer T, Kaya FA, et al. Interleukins 2, 6, and 8 levels in human gingival sulcus during orthodontic treatment. Am J Orthod Dentofacial Orthop 2006;130(1):7.e1–7.e6. DOI: 10.1016/j.ajodo.2005.12.027
  2. Tantilertanant Y, Niyompanich J, Everts V, et al. Cyclic tensile force-upregulated IL6 increases MMP3 expression by human periodontal ligament cells. Arch Oral Biol 2019;107:104495. DOI: 10.1016/j.archoralbio.2019.104495
  3. Madureira DF, da Silva JM, Teixeira AL, et al. Cytokine measurements in gingival crevicular fluid and periodontal ligament: are they correlated? Am J Orthod Dentofacial Orthop 2015;148(2):293–301. DOI: 10.1016/j.ajodo.2015.03.030
  4. Madureira DF, de Albuquerque Taddei S, Abreu MH, et al. Kinetics of interleukin-6 and chemokine ligands 2 and 3 expression of periodontal tissues during orthodontic tooth movement. Am J Orthod Dentofacial Oorthop 2012;142(4):494–500. DOI: 10.1016/j.ajodo.2012.05.012
  5. Davidovitch Z, Nicolay OF, Ngan PW, et al. Neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics. Dent Clin North Am 1988;32(3):411–435. DOI: 10.1155/2015/876509
  6. Shimizu N, Ogura N, Yamaguchi M, et al. Stimulation by interleukin-1 of interleukin-6 production by human periodontal ligament cells. Arch Oral Biol 1992;37(9):743–748. DOI: 10.1016/0003–9969(92)90081-i
  7. Feller L, Khammissa RA, Schechter I, et al. Biological events in periodontal ligament and alveolar bone associated with application of orthodontic forces. Sci World J 2015;2015. DOI: 10.1155/2015/876509
  8. Andrade Jr I, Taddei SR, Souza PE. Inflammation and tooth movement: the role of cytokines, chemokines, and growth factors. Semin Orthod WB Saunders 2012;18(4):257–269.
  9. Krishnan V, Davidovitch ZE. Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop 2006;129(4):469.e1–432.e1. DOI: 10.1016/j.ajodo.2005.10.007
  10. Meikle MC. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod 2006;28(3):221–240. DOI: 10.1093/ejo/cjl001
  11. Schröder A, Käppler P, Nazet U, et al. Effects of compressive and tensile strain on macrophages during simulated orthodontic tooth movement. Mediators Inflamm 2020;2020. DOI: 10.1155/2020/2814015
  12. Uematsu S, Mogi M, Deguchi T. Interleukin (IL)—1β, IL-6, tumor necrosis factor-α, epidermal growth factor, and β2-microglobulin levels are elevated in gingival crevicular fluid during human orthodontic tooth movement. J Dental Res 1996;75(1):562–567. DOI: 10.4103/jfmpc.jfmpc_204_19
  13. Zainal Ariffin SH, Yamamoto Z, Abidin Z, et al. Cellular and molecular changes in orthodontic tooth movement. Sci World J 2011;11. DOI: 10.1100/2011/761768
  14. Alhashimi N, Frithiof L, Brudvik P, et al. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofacial Orthop 2001;119(3):307–312. DOI: 10.1067/mod.2001.110809
  15. Mohammed A, Saidath K, Mohindroo A, et al. Assessment and measurement of interleukin 6 in periodontal ligament tissues during orthodontic tooth movement. World J Dent 2019;10(2):88–92. DOi: 10.5005/jp-journals-10015–1610
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