Flow cytometry is an invaluable tool to unravel the complexities of cell signaling. This article discusses the flow focusing principle behind most conventional cytometers and proposes an optimal Reynolds number range supported by published literature for increasing the accuracy of the data obtained by reducing doublets in the data. A description of the implementation of the microstructures in microfluidic cytometry chips for two-dimensional focusing along with an overview of its advantages has been presented along with how the proposed hypothesis can benefit this particle focusing scheme. Methods to test the given hypothesis have also been discussed.
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