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VOLUME 12 , ISSUE 5 ( September-October, 2021 ) > List of Articles


Association between Occupational Exposure to Tobacco Dust and Absolute Telomere Length: A Cross-sectional Study on Female Beedi Workers

Yamini Kanipakam, Vezhavendhan Nagaraja, Suganya Rajaram, Vidyalakshmi Santhanam, Sivaramakrishnan Muthanandam, Santha Devy Arumugam, Bala Maddileti Gandla, Raj Kumar Chinnadurai

Keywords : Occupational hazards, Real-time polymerase chain reaction, Telomere length, Tobacco dust

Citation Information : Kanipakam Y, Nagaraja V, Rajaram S, Santhanam V, Muthanandam S, Arumugam SD, Gandla BM, Chinnadurai RK. Association between Occupational Exposure to Tobacco Dust and Absolute Telomere Length: A Cross-sectional Study on Female Beedi Workers. World J Dent 2021; 12 (5):417-422.

DOI: 10.5005/jp-journals-10015-1854

License: CC BY-NC 4.0

Published Online: 29-09-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Aim and objective: The main aim of the study was to assess the absolute telomere length (aTL) in female beedi workers using real-time polymerase chain reaction (RT-PCR) and to compare the aTL with female non-beedi workers. Materials and methods: A cross-sectional study was carried out among age-matched 20 female non-beedi workers and 20 female beedi workers were enrolled for molecular analysis. The workers were in the age-group of 20–35 years and were workers exposed from 1 to 3 years. Saliva samples were collected from workers and control subjects for molecular analysis. The genomic DNA was extracted from saliva and aTL was estimated using real-time polymerase chain reaction. Results: The mean and standard deviation of average absolute TL/each chromosome end for the control group and study group were 0.75 ± 0.94 and 1.45 ± 2.76 kb. There was no statistically significant difference between the control group and the study group (Z = −0.112, p = 0.911). Conclusion: The present study revealed that there is no significant association in average absolute TL in early exposed female beedi workers when compared with female non-beedi workers. Furthermore, horizons are to be expanded for the population to prevent any occupational health hazards. Clinical significance: Telomere length is a biological clock that decides the lifetime of a cell and organism. Determination of TL is a better tool to detect genomic damage. Unburnt tobacco has been related to several health issues in beedi employees. The importance is to predict the genetic liability by estimating the aTL in beedi workers at early exposure to tobacco dust (TD).

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