World Journal of Dentistry

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VOLUME 12 , ISSUE 5 ( September-October, 2021 ) > List of Articles


Stability of Platelet-rich Fibrin Treated with Tranexamic Acid In Vivo: A Histological Study in Rats

Vellayappan Radha, Sheeja Saji Varghese, Mohanraj Karthik Ganesh

Keywords : Healing, Innovation, Platelet-rich fibrin, Regeneration, Tranexamic acid, Wistar rats

Citation Information : Radha V, Varghese SS, Ganesh MK. Stability of Platelet-rich Fibrin Treated with Tranexamic Acid In Vivo: A Histological Study in Rats. World J Dent 2021; 12 (5):386-391.

DOI: 10.5005/jp-journals-10015-1861

License: CC BY-NC 4.0

Published Online: 29-09-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Aim and objective: The present study aimed to evaluate the effect of tranexamic acid on the stability of platelet-rich fibrin (PRF) and its influence on connective tissue healing in Wistar rats. Materials and methods: This experimental study included 12 male Wistar rats in three groups; group I–control (saline), group II–untreated PRF, and group III–PRF treated with tranexamic acid (PRF + TXA). Platelet-rich fibrin membrane was prepared by drawing blood from each rat and the prepared membrane was cut into two halves wherein one half was treated with tranexamic acid. After anesthetizing the animal, a linear incision was made on the mid-posterior part of the dorsal side of the body to expose the muscle and three sites were prepared. Sterile saline was applied in the control site, untreated PRF membrane was placed in group II site, and PRF + TXA in group III site. Six rats were sacrificed at 14 days and the other six at 28 days. Specimens were subjected to histological evaluation for measuring the remaining PRF and immunohistochemical analysis for evaluating vimentin expression for assessing connective tissue healing. Results: Histopathological evaluation revealed that group III had more area of aggregation of PRF bundles at 28 days than group II. On immunohistochemical imaging, the intensity and proportionality score of vimentin expression was more in group III at 28 days. Conclusion: From the study, it can be concluded that tranexamic acid is effective in delaying the degradation of the PRF membrane and has a positive influence on connective tissue healing. Clinical significance: Improved stability of PRF observed with the addition of tranexamic acid will have wider applications in regenerative therapy using PRF.

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