Aim and objective: This study aimed to scrutinize sources and degree of stress in dental undergraduates in Asian Institute of Medicine, Science and Technology (AIMST) Dental Center during clinical years and correlating clinically with their oral hygiene. Materials and methods: A cross-sectional study was conducted among a total of 216 students including the third-, fourth-, and fifth-year dental students, and a modified version of the dental environment stress (DES) questionnaire was used to investigate the possible sources of stress during the clinical years. The results were evaluated using the statistical package for social sciences (SPSS) version 16. Basic descriptive statistics and Chi-square test were used to analyze and correlate the variables. A significant level was set at p < 0.05. Results: Total mean rank stress in terms of the academic year for the third year was 78.56, 125.6 in fourth, and 117.65 in the fifth year and was statistically higher for year 4 students. There is no statistical difference in the stress experienced by male and female undergraduate students. In response to the questionnaire pertaining to the source of stressors, an overall four-point Likert scale scoring of stress was marked as mild to moderate by the majority of the students. Pertaining to the level of oral hygiene to stress, statistical significance was noted with year 5 with a mean value higher for year 3 students. Conclusion: The overall findings of this investigation showed that dental students of AIMST University perceived comparatively mild-to-moderate levels of stress across the clinical study years. Clinical significance: Assessment of potential sources of stressors in the dental curriculum will point out the areas that need to be focused so that effective management can be instituted.