World Journal of Dentistry

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VOLUME 12 , ISSUE 1 ( January-February, 2021 ) > List of Articles

ORIGINAL RESEARCH

Analysis of Mast Cells Counts in Different Histopathological Grades of Leukoplakia and Squamous Cell Carcinoma of Oral Cavity

Kishor Patil

Keywords : Mast cells, Oral leukoplakia, Oral squamous cell carcinoma

Citation Information : Patil K. Analysis of Mast Cells Counts in Different Histopathological Grades of Leukoplakia and Squamous Cell Carcinoma of Oral Cavity. World J Dent 2021; 12 (1):74-77.

DOI: 10.5005/jp-journals-10015-1795

License: CC BY-NC 4.0

Published Online: 00-02-2021

Copyright Statement:  Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim and objective: The aim and objective of this study was to estimate mast cell (MC) count in histopathological dysplasia grades in oral leukoplakia (OL) patients and in histopathological grades in oral squamous cell carcinoma (OSCC) patients and thereby determining their possible role in these lesions pathogenesis. Materials and methods: Forty histopathologically confirmed cases of OL and OSCC were taken. Sections of 4 microns thickness were taken from each paraffin block and stained with 1% standard toluidine blue stain for demonstrating MCs. Counting of MCs was done by 3 observers using a 10× eyepiece fitted with 1 cm2 graticule and 40× objectives in 10 different fields in each slide. Results: It was found that the MC count was higher in OSCC than OL and control group and the difference was found to be statistically significant. (One way Analysis of Variance test) Also, the MC count increased from mild to severe dysplasia in OL patients, as well as from well to poorly differentiated grades in OSCC patients (p < 0.01). Conclusion: This study indicates that MC count was increased from mild to moderate to severe dysplasia of OL patients and also from well differentiated (WD) to moderately differentiated (MD) to poorly differentiated (PD) grades of OSCC patients. Clinical significance: MC count was increased in higher grades of epithelial dysplasia in OL patients and higher grades in OSCC patients.


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