Aim: Squamous cell carcinoma (OSCC) is the most common malignancy occurring in the oral cavity. It causes local invasion and nodal metastasis leading to an increase in morbidity rate. Vascular endothelial growth factor (VEGF)-C has been the most widely studied molecule involved in lymphangiogenesis. The aim is to study the expression of VEGF-C in OSCC using the immunohistochemical (IHC) method and is correlated with nodal metastasis.
Materials and methods: The study group consisted of 49 clinically and histopathologically diagnosed cases of OSCC. The tissue sections were subjected to IHC staining with anti-VEGF-C antibody and assessed for its expression and are correlated with the lymph node metastasis.
Results: Out of 49 cases, 26 cases showed lymph node metastasis. On Chi-square analysis, the expression of VEGF-C was statistically significant with lymph node metastasis (p < 0.05). But, the expression of VEGF-C with clinicopathological staging and grading was not significant.
Conclusion: The present study results suggest VEGF-C can be used as an indicator marker in lymph node metastasis.
Clinical significance: Vascular endothelial growth factor-C marker can be recommended for the prediction of lymph node metastasis in oral squamous cell carcinoma.
Sugiura T, Inque Y, Matsuki R, et al. VEGF-C and VEGF-D expression is correlated with lymphatic vessel density and lymph node metastasis in oral squamous cell carcinoma: implications for use as a prognostic marker. Int J Oncol 2009;34(3):673–680. DOI: 10.3892/ijo_00000193.
Siriwardena BSMS, Kudo Y, Ogawa I, et al. VEGF-C is associated with lymphatic status and invasion in oral cancer. J Clin Pathol 2008;61(1):103–108. DOI: 10.1136/jcp.2007.047662.
Zhang Z, Joseph I, Helman JI, et al. Lymphangiogenesis, lymphatic endothelial cells and lymphatic metastasis in head and neck cancer — a review of mechanisms. Int J Oral Sci 2010;2(1):5–14. DOI: 10.4248/IJOS10006.
Franchi A, Gallo O, Massi D, et al. Tumor lymphangiogenesis in head and neck squamous cell carcinoma A morphometric study with clinical correlations. Cancer 2004;101(5):973–978. DOI: 10.1002/cncr.20454.
Miyahara M, Tanuma J, Sugihara K, et al. Tumor lymphangiogenesis correlates with lymph node metastasis and clinicopathologic parameters in oral squamous cell carcinoma. Cancer 2007;110(6):1287–1294. DOI: 10.1002/cncr.22900.
Soini Y, Puhakka A, Kahlos K, et al. Endothelial nitric oxide synthase is strongly expressed in malignant mesothelioma but does not associate with vascular density or the expression of VEGF, FLK1 or FLT1. Histopathol 2001;39(2):179–186. DOI: 10.1046/j.1365-2559.2001.01211.x.
Markopoulos KA. Current aspects on oral squamous cell carcinoma. The Open Dentis J 2012;6:126–130.
Tandon P, Dadhich A, Saluja H, et al. The prevalence of squamous cell carcinoma in different sites of oral cavity at our rural health care centre in Loni, Maharashtra – a retrospective 10-year study. Contemp Oncol (Pozn) 2017;21(2):178–183. DOI: 10.5114/wo.2017.68628.
Singh MP, Kumar V, Agarwal A, et al. Clinico-epidemiological study of oral squamous cell carcinoma: a tertiary care centre study in North India. J Oral Biol Craniofac Res 2016;6(1):31–34. DOI: 10.1016/j.jobcr.2015.11.002.
Coelho KR. Challenges of the oral cancer burden in India. J Cancer Epidemiol 2012. 1–17. DOI: 10.1155/2012/701932.
Selvi UPG, Kamatchi D, Tharani R, et al. Demographic, habits and clinical presentation of oral cancer in Trichy's population. IJCMR 2017;4(1):287–290.
Kishimoto K, Sasaki A, Yoshihama Y, et al. Expression of vascular endothelial growth factor-C predicts regional lymph node metastasis in early oral squamous cell carcinoma. Oral Oncol 2003;39(4):391–396. DOI: 10.1016/S1368-8375(02)00143-4.
Sedivy R, Beck-Mannagetta J, Haverkampf C, et al. Expression of vascular endothelial growth factor-C correlates with the lymphatic microvessel density and the nodal status in oral squamous cell cancer. J Oral Pathol Med 2003;32(8):455–460. DOI: 10.1034/j.1600-0714.2003.00168.x.
Shintani S, Li C, Ishikawa T, et al. Expression of vascular endothelial growth factor A, B, C, and D in oral squamous cell carcinoma. Oral Oncol 2004;40(1):13–20. DOI: 10.1016/S1368-8375(03)00127-1.
Sahaf R, Aman-ur-Rehman, Hussain S, et al. Expression of vascular endothelial growth factor-C in oral squamous cell carcinoma: an immunohistochemical study. Biomed J Sci Tech Res 2018;2(1):1–6. DOI: 10.26717/BJSTR.2017.01.000626.
Naruse T, Kawasaki G, Yanamoto S, et al. Immunohistochemical study of VEGF expression in oral squamous cell carcinomas: correlation with the mTOR-HIF-1α pathway. Anticancer Res 2011;31(12):4429–4437.
Matsui T, Shigeta T, Umeda M, et al. Vascular endothelial growth factor C (VEGF-C) expression predicts metastasis in tongue cancer. Oral Surg, Oral Med, Oral Pathol Oral Radiol 2015;120(4):436–442. DOI: 10.1016/j.oooo.2015.06.002.
Faustino SES, Oliveira DT, Nonogaki S, et al. Expression of vascular endothelial growth factor-C does not predict occult lymph-node metastasis in early oral squamous cell carcinoma. Int J Oral Maxillofac Surg 2008;37(4):372–378. DOI: 10.1016/j.ijom.2007.11.021.
Yanase M, Kato K, Yoshizawa K, et al. Prognostic value of vascular endothelial growth factors A and C in oral squamous cell carcinoma. J Oral Pathol Med 2014;43(7):514–520. DOI: 10.1111/jop.12167.
Kitadai Y, Amioka T, Haruma K, et al. Clinicopathological significance of vascular endothelial growth factor (VEGF)-C in human esophageal squamous cell carcinomas. Int J Cancer 2001;93(5):662–666. DOI: 10.1002/ijc.1379.
Omoto I, Matsumoto M, Okumura H, et al. Expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 in esophageal squamous cell carcinoma. Oncol Lett 2014;7(4):1027–1032. DOI: 10.3892/ol.2014.1823.
Han FH, Li HM, Zheng DH, et al. The effect of the expression of vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 on the clinical outcome in patients with gastric carcinoma. Eur J Surg Oncol 2010;36(12):1172–1179. DOI: 10.1016/j.ejso.2010.09.002.
Witte D, Thomas A, Ali N, et al. Expression of the vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligand VEGF-C in human colorectal adenocarcinoma. Anticancer Res 2002;22:1463–1466.
Arinaga M, Noguchi T, Takeno S, et al. Clinical significance of vascular endothelial growth factor C and vascular endothelial growth factor receptor 3 in patients with non small cell lung carcinoma. Cancer 2003;97(2):457–464. DOI: 10.1002/cncr.11073.
Singh P, Augustine D, Rao RS, et al. Interleukin-1beta and Caspase-3 expression serve as independent prognostic markers for metastasis and survival in oral squamous cell carcinoma. Cancer Biomark 2019;26(1):109–122. DOI: 10.3233/CBM-190149.