World Journal of Dentistry

Register      Login

VOLUME 11 , ISSUE 2 ( March-April, 2020 ) > List of Articles

ORIGINAL RESEARCH

An In Vitro Assessment of Physicomechanical Properties of Heat-cured Denture Base Resin Disinfected by Ozonized Water

Reham M Abdallah, Neven S Aref

Keywords : Flexural strength, Microhardness, Ozone, Polymethyl methacrylate, Surface roughness

Citation Information : Abdallah RM, Aref NS. An In Vitro Assessment of Physicomechanical Properties of Heat-cured Denture Base Resin Disinfected by Ozonized Water. World J Dent 2020; 11 (2):146-150.

DOI: 10.5005/jp-journals-10015-1718

License: CC BY-NC 4.0

Published Online: 01-08-2020

Copyright Statement:  Copyright © 2020; The Author(s).


Abstract

Aim: This study investigates the influence of ozonized water disinfection on flexural strength, surface roughness, and surface microhardness of heat-cured denture base material [(polymethyl methacrylate (PMMA)]. Materials and methods: A total number of 90 specimens were prepared from heat-cured denture base material. In the control group (n = 30), 10 specimens from each test were immersed only in distilled water at 37°C for 48 hours before testing. For the two experimental groups (n = 60), 10 specimens of each group in each test were immersed in 2% chlorhexidine for 10 minutes and another 10 specimens were immersed in ozonized water with a concentration of 10 mg/L for 30 minutes. In the flexural strength test, specimens were subjected to three-point loading at a crosshead speed of 5 mm/minute of a universal testing machine. Hardness measurements using Vickers microhardness tester and roughness measurements by the Surftest analyzer were performed. Measurements of flexural strength, surface roughness (Ra, μm), and hardness (kg/mm2) were analyzed using one-way analysis of variance (ANOVA) and Tukey least significant difference (LSD) test (α = 0.05). Results: Flexural strength values of ozonized water-disinfected specimens were insignificantly decreased. However, the use of ozonized water disinfection significantly increased roughness values. At the same time, microhardness values significantly decreased. Conclusion: The use of ozonized water in disinfecting heat-cured denture base resin did not exhibit a deleterious effect on its strength nor surface roughness. Thus, it may be a much more safe disinfection method rather than chlorhexidine chemical disinfectant. Clinical significance: Disinfection of heat-cured PMMA denture base resin using ozonized water may be a more valuable hygienic method compared to chlorhexidine, the most common chemical disinfectant.


HTML PDF Share
  1. Kossioni AE. The prevalence of denture stomatitis and its predisposing conditions in an older greek population. Gerodontology 2011;28(2):85–90. DOI: 10.1111/j.1741-2358.2009.00359.x.
  2. Dills SS, Olshan AM, Goldner S, et al. Comparison of the antimicrobial capability of an abrasive paste and chemical soak denture cleaners. J Prosthet Dent 1988;60(4):467–470. DOI: 10.1016/0022-3913(88)90250-8.
  3. Jagger DC, Harrison A. Denture cleansing–the best approach. Braz Dent J 1995;178(11):413–417. DOI: 10.1038/sj.bdj.4808788.
  4. Glass RT, Goodson LB, Bullard JW, et al. Comparison of the effectiveness of several denture sanitizing systems: a clinical study. Compend Contin Educ Dent 2001;22(12):1093–1096.
  5. Harrison Z, Johnson A, Douglas CW. An in vitro study into the effect of a limited range of denture cleaners on surface roughness and removal of candida albicans from conventional heat-cured acrylic resin denture base material. J Oral Rehabil 2004;31(5):460–467. DOI: 10.1111/j.1365-2842.2004.01250.x.
  6. Brace ML, Plummer KD. Practical denture disinfection. J Prosthet Dent 1993;70(6):538–540. DOI: 10.1016/0022-3913(93)90268-s.
  7. Oliveira LV, Mesquita MF, Henriques GE, et al. The effect of brushing on surface roughness of denture lining materials. J Prosthodont 2007;16(3):179–184. DOI: 10.1111/j.1532-849X.2006.00169.x.
  8. Arita M, Nagayoshi M, Fukuizumi T, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol 2005;20(4):206–210. DOI: 10.1111/j.1399-302X.2005.00213.x.
  9. Hems RS, Gulabivala K, Ng YL, et al. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Inter Endod J 2005;38(1):22–29. DOI: 10.1111/j.1365-2591.2004.00891.x.
  10. Baysan A, Whiley RA, Lych E. Antimicrobial effect of a novel ozone-generating device on microorganisms associated with primary root carious lesions in vitro. Caries Res 2000;34(6):498–501. DOI: 10.1159/000016630.
  11. Velano HE, Nascimento LC, Barros LM, et al. In vitro evaluation of the antibacterial activity of ozonized water against Staphylococcus aureus. Pesq Odontol Bras 2001;15(1):18–22. DOI: 10.1590/S1517-74912001000100004.
  12. Murakami H, Sakuma S, Nakamura K, et al. Disinfection of removable dentures using ozone. Dent Mater J 1996;15(2):220–225. DOI: 10.4012/dmj.15.220.
  13. Oizumi M, Suzuki T, Uchida M, et al. In-vitro testing of a denture cleaning method using ozone. J Med Dent Sci 1998;45(2): 135–139.
  14. Baley JE. Neonatal candidiasis: the current challenge. Clin Perinatol 1991;18(2):263–279. DOI: 10.1016/S0095-5108(18)30523-2.
  15. Saballs P, Towers-Rodrigues JM, Saved M. La candidemia in acquired immunodeficiency syndrome. Retrospect Study Nine Cases Rev Iberoam Micol 2000;17:2–5.
  16. Sandvén P. Laboratory identification and sensitivity testing of yeast isolates. Acta Odontol Scand 1990;48(1):27–36. DOI: 10.3109/00016359009012731.
  17. Julio C, Tânia C, Juliana C, et al. Antimicrobial effects of ozonated water on the sanitization of dental instruments contaminated with E. coli, S. aureus, C. albicans, or the spores of B. atrophaeus. J Infect Pub Health 2012;5(4):269–274. DOI: 10.1016/j.jiph.2011.12.007.
  18. Bezirtzoglou E, Cretoiu SM, Moldoveanu M, et al. A quantitative approach to the effectiveness of ozone against microbiota organisms colonizing tooth brushes. J Dent 2008;36(8):600–605. DOI: 10.1016/j.jdent.2008.04.007.
  19. Huth KC, Jakob FM, Saugel B, et al. Hollweck ret al. effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006;114(5):435–440. DOI: 10.1111/j.1600-0722.2006.00390.x.
  20. Shen C, Javid NS, Colaizzi FA. The effect of glutaraldehyde base disinfectants on denture base resins. J Prosthet Dent 1989;61(5):583–589. DOI: 10.1016/0022-3913(89)90281-3.
  21. Carvalho CF, Vanderlei AD, Marocho SM, et al. Effect of disinfectant solutions on a denture base acrylic resin. Acta Odontol 2012;25(3):255–260.
  22. Schmidlin PR, Zimmermann J, Bindl A. Effect of ozone on enamel and dentin bond strength. J Adhes Dent 2005;7(1):29–32.
  23. Neppelenbroek KH, Pavarina AC, Vergani CE, et al. Hardness of heat-polymerized acrylic resins after disinfection and long-term water immersion. J Prosthet Dent 2005;93(2):171–176. DOI: 10.1016/j.prosdent.2004.10.020.
  24. Durkan R, Ayaz EA, Bagis B, et al. Comparative effects of denture cleansers on physical properties of polyamide and polymethyl methacrylate base polymers. Dent Mater J 2013;32(3):367–375. DOI: 10.4012/dmj.2012-110.
  25. Vallittu PK, Miettinen V, Alakuijala P. Residual monomer content and its release into water from denture base materials. Dent Mater 1995;11(6):338–342. DOI: 10.1016/0109-5641(95)80031-x.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.