World Journal of Dentistry

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VOLUME 11 , ISSUE 1 ( January-February, 2020 ) > List of Articles


Antifungal Activity of Aloe Vera Leaf and Gel Extracts Against Candida albicans: An In Vitro Study

M Shilpa, Vinaya Bhat, A Veena Shetty, Mora SR Reddy, Prashant Punde

Keywords : Aloe vera, Antifungal, Candida albicans, Natural

Citation Information : Shilpa M, Bhat V, Shetty AV, Reddy MS, Punde P. Antifungal Activity of Aloe Vera Leaf and Gel Extracts Against Candida albicans: An In Vitro Study. World J Dent 2020; 11 (1):36-40.

DOI: 10.5005/jp-journals-10015-1701

License: CC BY-NC 4.0

Published Online: 25-10-2012

Copyright Statement:  Copyright © 2020; The Author(s).


Aim: The present study was conducted with an aim to assess the antimicrobial activity of ethanolic extracts of aloe vera leaf and gel against Candida albicans in vitro. Materials and methods: Fresh leaves were collected from the aloe vera plants naturally grown in Coorg. Aloe vera leaf as well as gel was separated, extracted with 95% ethanol in rotary shaker at constant temperature for 3 days, evaporated in a heating mantle, and stored in screw cap test tubes at 4°C for further analysis. Antifungal activity of aloe vera leaf and gel extracts against C. albicans was assessed by well diffusion method. Further, gel extracts of aloe vera at different dilutions (500, 400, 300, and 200 μL) were prepared and the turbidity was analyzed. Results: Results showed that the ethanolic extract of aloe vera leaf did not show antifungal activity against C. albicans. A maximum of 99.33% antifungal activity was shown by 400 μL of aloe vera gel extract. The minimum inhibitory concentration of aloe vera gel extract was 200 μL (98.2% inhibition). Conclusion: The ethanolic extract of aloe vera gel showed considerable antifungal activity against C. albicans. Clinical significance: Modalities targeted on the use of aloe vera against C. albicans can prove to be more beneficial and consistent compared to conventional antifungals for preventive and/or therapeutic purposes against a variety of oral fungal diseases.

  1. Farahnejad Z, Ghazanfari T, Yaraee R. Immunomodulatory effects of Aloe vera and its fractions on response of macrophages against Candida albicans. Immunopharmacol Immunotoxicol 2011;33(4): 676–681. DOI: 10.3109/08923973.2011.560158.
  2. Raksha B, Pooja S, Babu S. Bioactive compounds and medicinal properties of Aloe vera L.: an update. J Plant Sci 2014;2(3):102–107.
  3. Sharma A, Gautam S. An overview on medicinal properties of Aloe vera: antibacterial & antifungal aspects. Int J Pharm Bio Sci 2013;4(3):694–705.
  4. Gupta VK, Malhotra S. Pharmacological attribute of Aloe vera: revalidation through experimental and clinical studies. Ayu 2012;33(2):193–196. DOI: 10.4103/0974-8520.105237.
  5. Shireen F, Manipal S, Prabu D. Anti-fungal activity of Aloe vera: in vitro study. SRM J Res Dent Sci 2015;6(2):92–95. DOI: 10.4103/0976-433X.155464.
  6. Doddanna SJ, Patel S, Sundarrao MA, et al. Antimicrobial activity of plant extracts on Candida albicans: an in vitro study. Indian J Dent Res 2013;24(4):401–405. DOI: 10.4103/0970-9290.118358.
  7. Hegde V, Rodrigues SJ, Saldanha S. Redefining the treatment modalities for denture stomatitis- a review. Int J Res Dent 2014;4(2): 8–27.
  8. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, et al. Development of candida-associated denture stomatitis: new insights. J Appl Oral Sci 2008;16(2):86–94. DOI: 10.1590/s1678-77572008000200002.
  9. Shah N, Madhu KS, Sreenivasa Murthy BV, et al. Identification of presence of Candida albicans in primary root canal infections: an in vitro study. Endodontology 2016;28(2):109–113. DOI: 10.4103/0970-7212.195440.
  10. Khaing TA. Evaluation of the antifungal and antioxidant activities of the leaf extract of Aloe vera (Aloe barbadensis miller). Proc World Acad Sci, Eng Tech 2011;75:610–612.
  11. Agarry OO, Olaleye MT, Bello-Michael CO. Comparative antimicrobial activities of aloe vera gel and leaf. Afr J Biotechnol 2005;4:1411–1414.
  12. Tamilarasi L, Ponnulakshmi R, Ezhilarasi BS. Comparative study on the antifungal activity of Aloe vera leaf and gel in different extracts. Int J Pharm Bio Sci 2014;5(2):B895–B901.
  13. Devi DL, Srinivas B, Rao BN. An evaluation of antimicrobial activity of Aloe barbadensis miller (Aloe vera) gel extract. JPBMS 2012;21(03):1–4.
  14. Renisheya T, Johnson M, Nancy BS, et al. Anti-bacterial and antifungal activity of aloe veragel extract. IJBAR 2012;03(03):184–187.
  15. Stanley MC, Ifeanyi OE, Eziokwu OG. Antimicrobial effects of aloe vera on some human pathogens. Int J Curr Microbiol App Sci 2014;3(3):1022–1028.
  16. Bhat G, Kudva P, Dodwad V. Aloe vera: Nature’s soothing healer to periodontal disease. J Indian Soc Periodontal 2011;15(3):205–209. DOI: 10.4103/0972-124X.85661.
  17. Sundarkar P, Govindwar R, Nyamati SB, et al. Use of aloe vera in dentistry. J Indian Acad Oral Med Radiol 2011;23(3):S389–S391.
  18. Tayal E, Sardana D, Indushekar KR, et al. Current perspectives on use of aloe vera in dentistry. Eur J Med Plants 2014;4(12):1408–1419. DOI: 10.9734/EJMP/2014/10843.
  19. Agarwal A, Dwivedi N. Aloe vera: magic or myth. SRM J Res Dent Sci 2013;4(3):119–124. DOI: 10.4103/0976-433X.121638.
  20. Isadkar YS, Palaskar SJ, Narang B, et al. Aloe vera as denture cleanser. J Dent Allied Sci 2018;7(1):23–26. DOI: 10.4103/jdas.jdas_44_17.
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