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VOLUME 8 , ISSUE 1 ( January-February, 2017 ) > List of Articles

RESEARCH ARTICLE

Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres

Ravindra Kotian, P Prasad Rao, Prashanthi Madhyastha, KL Shobha, BS Satish Rao

Citation Information : Kotian R, Rao PP, Madhyastha P, Shobha K, Rao BS. Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World J Dent 2017; 8 (1):28-36.

DOI: 10.5005/jp-journals-10015-1406

Published Online: 01-06-2013

Copyright Statement:  Copyright © 2017; The Author(s).


Abstract

Aim

This study was performed to evaluate the biocompatibility of pure titanium and Ti-6Al-4V metals coated with hydroxyapatite (HA) by plasma spray using different plasma gas atmospheres.

Materials and methods

The cell viabilities for each HA-coated sample in an atmosphere of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen were studied using MTT assay and platelet adhesion test.

Results

The mean cell viabilities by MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] assay of samples coated with HA in argon—hydrogen plasma atmosphere showed maximum cell viability at different time intervals compared with other coating atmospheres of argon—hydrogen, nitrogen, and nitrogen—hydrogen. A statistically significant value of cell viability (p < 0.001) was observed between and within the groups of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen plasma gas atmosphere. The platelet adhesion study showed agglomerates of platelet cells in some isolated regions of HA for all atmospheres.

Significance

The results obtained in this study can serve as a guide for the development of new Ti-based HA-coated implants in different plasma gas atmospheres.

How to cite this article

Kotian R, Rao PP, Madhyastha P, Shobha KL, Rao BSS, Ginjupalli K. Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World J Dent 2017;8(1):28-36.


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