In Vitro Cytotoxic Evaluation of Mineral Trioxide Aggregate with Silver and Titanium Dioxide Nanoparticles

Teena Sheethal Dsouza; Vishakh Radhakrishna; Neevan Dsouza; Suchetha Kumari

doi:10.5005/jp-journals-10015-1684

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In Vitro Cytotoxic Evaluation of Mineral Trioxide Aggregate with Silver and Titanium Dioxide Nanoparticles

Teena Sheethal Dsouza, Vishakh Radhakrishna, Neevan Dsouza, Suchetha Kumari

Keywords : Cytotoxicity, Lymphocytes, Mineral trioxide aggregate, MTT assay, Nanoparticle, Silver, Titanium dioxide

Citation Information : Dsouza TS, Radhakrishna V, Dsouza N, Kumari S. In Vitro Cytotoxic Evaluation of Mineral Trioxide Aggregate with Silver and Titanium Dioxide Nanoparticles. World J Dent 2019; 10 (6):432-434.

DOI: 10.5005/jp-journals-10015-1684

License: CC BY-NC 4.0

Published Online: 01-12-2016

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Abstract

Aim: The aim of the study was to evaluate the cytotoxicity of mineral trioxide aggregate (MTA) when mixed with either silver or titanium dioxide (TiO₂) nanoparticles on human lymphocytes. Materials and methods: Human lymphocytes were exposed to white MTA or MTA in combination with silver or TiO₂ nanoparticles. The cytotoxicity was assessed using the MTT assay for freshly mixed pellets, at 24 hours, at 48 hours, and at 72 hours time periods. The results were statistically analyzed. Results: There was no significant difference in the experimental groups with regard to cell viability. The addition of nanoparticles to MTA showed similar cell viability compared with MTA alone. Conclusion: Mineral trioxide aggregate mixed with silver or TiO₂ nanoparticles showed similar biocompatibility to MTA alone. Clinical significance: The cytotoxicity exhibited by silver or TiO₂ nanoparticles when mixed with MTA showed no significant difference and therefore, they can be used as a suitable additive to improve the antimicrobial efficacy of MTA.

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