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VOLUME 9 , ISSUE 6 ( November-December, 2018 ) > List of Articles


Increase of Enamel Hardness Score after Cow Milk Immersion of Demineralized Tooth: An In vitro Study

Yendriwati, Rizka M Sinaga, D Dennis

Keywords : Artificial saliva, Cow milk, Enamel hardness, Remineralization

Citation Information : Y, Sinaga RM, Dennis D. Increase of Enamel Hardness Score after Cow Milk Immersion of Demineralized Tooth: An In vitro Study. World J Dent 2018; 9 (6):439-443.

DOI: 10.5005/jp-journals-10015-1577

License: CC BY-NC 4.0

Published Online: 01-12-2018

Copyright Statement:  Copyright © 2018; The Author(s).


Aim: To investigate the difference of enamel hardness score on tooth immersed in artificial saliva and cow milk. Materials and methods: The present study was an experimental laboratory with pre- and post-test design. Thirty-two maxillary first premolars were collected and randomly divided into two groups, with 16 samples each immersed in artificial saliva and cow milk. Samples were immersed in an orange flavored soft drink for 5 minutes before exposing to cow milk (group 1) or artificial saliva (group 2) for 5 minutes twice a day until day 3. Enamel hardness score was measured as follows: pretreatment, post demineralization stage and posttreatment at day 1 and day 3 by Micro Vickers hardness tester. Data were analyzed using paired t-test and one way analysis of variance (ANOVA). Results: There was a significant difference (p value = 0.000) on enamel hardness score of group 1 at day 1 (324.39 ± 20.35 VHN) and day 3 (354.80 ± 21.09 VHN) as well as group 2 at day 1 (308.06 ± 15.94 VHN) and day 3 (322.18 ± 16.94 VHN). Tooth enamel hardness on group 1 was significantly different to group 2 both at day 1 (p value = 0.018) and day 3 (p value=0.000). Conclusion: These results suggest that artificial saliva and cow milk can increase the enamel hardness score. However, the efficacy of cow milk to augment the tooth enamel hardness is much higher than that of artificial saliva. Clinical significance: Can be used as a primary advice to the public in as precautionary measures and inhibiting tooth enamel damage from acidic drinks.

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  1. Lachowski KM, Ferreira D, de Oliveira TA, Sobral MAP. Effect of the mixture of coffee or chocolate to milk in the progression of des-remineralization of tooth enamel – an in vitro study. Braz Research in Pediatric Dent and Integrated Clinic 2014;14(3):183-190.
  2. Miller KR. Remineralization strategies. http://www.rdhmag. com/articles/print/volume-26/issue7/feature/remineralization- strategies.html (8 October 2015).
  3. Yamamoto ETC, Vanderlei A, Amaral R, Nicolo RD, da Rocha JC, de Araujo MAM. Influence of three types of drinks on the surface of human dental enamel: in vitro study. Rev Gaucha- Odontol 2013;61(1):42.
  4. McCabe JF, Walls AWG. Bahankedokterangigi. AlihBahasa. Sunarinstyas S, Mustaqimah DN. Jakarta: EGC, 2015: 19-20.
  5. Gutierrez-Salazar MDP, Reyes-Gasga J. Microhardness and chemical composition of human tooth. Material Research 2003;6(3):367-373.
  6. Owens BM, Malette JD, Phebus JG. Effect of carbonated cola beverages, sports and energy drinks and orange juice on primary and permanent enamel dissolution. Austin J Dent 2014;1:1-2.
  7. Widyaningtyas V, Rahayu YC, Barid I. Analisispeningkatanremineralisasi enamel gigisetelahdirendamdalamsusukedelaimurni (Glycine max (L.) merill) menggunakan scanning electron microscope (SEM). J Pustaka Kesehatan 2014;2: 258-261.
  8. Stegeman CA, Davis JR. The dental hygienist's guide to nutritional care. 3 th ed., Missouri: Saunders Elsevier, 2010:146.
  9. Amoras DR, Corona SA, Rodrigues Jr AL, Serra MC. Effect of beverages on bovine dental enamel subjected to erosive challenge with hydrochloric acid. Brazilian dental journal. 2012;23(4):367-372.
  10. Palti DG, Machado MAAM, Silva SMB, Abdo RCC, Lima JEO. Evaluation of superficial microhardness in dental enamel with different eruptive ages. Braz Oral Res, 2008;22(4):313-314.
  11. Collin FM. Treatment options for tooth discoloration and remineralization. Pennwell, 2008:5.
  12. Hemagaran G, Neelakantan P. Remineralization of the tooth structure–the future of dentistry. Int J of Pharm Tech Research 2014;6(2):487-491.
  13. Davari AR, Kazemi ARD, Ataei E, Vatanpour M, Abdollahi H. Effects of bleaching and remineralising agents on the surface hardness of enamel. J Dent Shiraz Univ Med Scien 2012;13(4):157-158.
  14. Telgi RL, Yadav V, Telgi CR, Boppana N. In vivo dental plaque pH after consumption of dairy products. General Dentistry 2013.56-57.
  15. Zafar S, Harnekar SY, Siddiqi A. Early childhood caries: etiology, clinical considerations, consequences andand management. International Dentistry SA 2009;11(4):24-36.
  16. Wang X, Mihailova B, Klocke A, Heidrich S, Bismayer U. Effect of artificial saliva on the apatite structure of eroded enamel. Internatinal J of Spectroscopy 2011;2011
  17. Reema SD, Lahiri PK, Roy SS. Review of casein phosphopeptides- amorphous calcium phosphate. The Chinese Journal of Dental Research, 2014;17(1):7-14.
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