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VOLUME 9 , ISSUE 3 ( May-June, 2018 ) > List of Articles

ORIGINAL RESEARCH

Scanning Electron Microscopic Evaluation of Smear Layer Removal Ability of Novel Irrigant QMix compared with Others at Apical Third of the Root

Sreeha K Kolanu, Bolla Nagesh, Phani HD Vaaka, Swapnika Polineni

Keywords : Laboratory research, MTAD, QMix, Root canal irrigation, Scanning electron microscopy, Smear layer, Tubulicid Plus

Citation Information : Kolanu SK, Nagesh B, Vaaka PH, Polineni S. Scanning Electron Microscopic Evaluation of Smear Layer Removal Ability of Novel Irrigant QMix compared with Others at Apical Third of the Root. World J Dent 2018; 9 (3):220-224.

DOI: 10.5005/jp-journals-10015-1537

License: CC BY-SA 4.0

Published Online: 00-06-2018

Copyright Statement:  Copyright © 2018; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: The success of an endodontic treatment depends on the complete elimination of the microorganisms from the root canal system for which effective eradication of smear layer is crucial. Hence, various final irrigating solutions were introduced in endodontics. The aim of this in vitro study is to compare the smear layer removal efficacy of different final irrigating solutions at the apical third of the root canal through scanning electron microscopic (SEM) image analysis. Materials and methods: Forty human single-rooted mandibular premolar teeth were taken and decoronated to standardize the canal length. Each tooth was prepared endodontically with same chemomechanical technique and the teeth were randomly divided into four groups based on the final irrigation regimen. Group I: saline, group II: Tubulicid Plus, group III: BioPure MTAD, and group IV: QMix. After final irrigation, SEM evaluation was done for the assessment of removal of the smear layer at apical third, and data were analyzed using the Kruskal–Wallis test and Mann–Whitney U test. Results: Intergroup comparison of the groups showed statistically significant differences in the smear layer removal efficacy of irrigating solutions tested. QMix is most effective in the removal of smear layer when compared with other tested irrigating solutions. Conclusion: QMix is the most effective final irrigating solution for smear layer removal followed by MTAD and Tubulicid Plus. Clinical significance: Final irrigating solution plays a major role in the elimination of smear layer, thereby enhancing the hermetic seal of obturating materials, resulting in greater success rate of root canal treatment. Further research is required regarding the use of QMix on smear layer removal and also its effect on ultrastructural changes in dentin.


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  1. Violich DR, Chandler NP. The smear layer in endodontics—a review. Int Endod J 2010 Jan;43(1):2-15.
  2. Heard F, Walton RE. Scanning electron microscope study comparing four root canal preparation techniques in small curved canals. Int Endod J 1997;30:323-331.
  3. Pashley DH. Smear layer: overview of structure and function. Proc Finn Dent Soc 1992;88 (Suppl 1):215-224.
  4. Economides N, Liolios E, Kolokuris I, Beltes P. Long-term evaluation of the influence of smear layer removal on the sealing ability of different sealers. J Endod 1999 Feb;25(2): 123-125.
  5. Saunders WP, Saunders EM. Influence of smear layer on the coronal leakage of thermafil and laterally condensed guttapercha root fillings with a glass ionomer sealer. J Endod 1994 Apr;20(4):155-158.
  6. Brannstrom M. Smear layer: pathological and treatment considerations. Oper Dent (Suppl) 1984;3:35-42.
  7. Pashley DH. Smear layer: physiological considerations. Oper Dent (Suppl) 1984;3:13-29.
  8. Torabinejad M, Handysides R, Khademi AA, Bakland LK. Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002 Dec;94(6):658-666.
  9. Torabinejad M, Khademi AA, Babagoli J, Cho Y, Johnson WB, Bozhilov K, Kim J, Shabahang S. A new solution for the removal of the smear layer. J Endod 2003 Mar;29(3):170-175.
  10. Kara Tuncer A, Tuncer S, Siso SH. Effect of Qmix irrigant on microhardness of dentine. Aust Dent J 2015 Jun;60(2):163-168.
  11. McComb D, Smith DC. A preliminary scanning electron microscopic study of rootcanals after endodontic procedures. J Endod 1975 Jul;1(7):238-242.
  12. Moodnik RM, Dorn SO, Feldman MJ, Meadow E, Levely M, Borden BG. Efficacy of biomechanical instrumentation: a scanning electron microscopic study. J Endod 1976 Sep;2(9): 261-266.
  13. Lui JN, Kuah HG, Chen NN. Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. J Endod 2007;33:472-475.
  14. Kuah HG, Lui JN, Tseng PS, Chen NN. The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod 2009;35:393-396.
  15. Chandrasekhar V, Amulya V, Rani VS, Prakash TJ, Ranjani AS, Gayathri Ch. Evaluation of biocompatibility of a new root canal irrigant QMix™ 2 in 1—an in vivo study. J Conserv Dent 2013 Jan;16(1):36-40.
  16. Eliot C, Hatton JF, Stewart GP, Hildebolt CF, Jane Gillespie M, Gutmann JL. The effect of the irrigant QMix on removal of canal wall smear layer: An ex vivo study. Odontology 2014 Jul;102(2):232-240.
  17. Stojicic S, Shen Y, Qian W, Johnson B, Haapasalo M. Antibacterial and smear layer removal ability of a novel irrigant, QMiX. Int Endod J 2012;45:363-371.
  18. Ballal NV, Tweeny A, Khechen K, Prabhu KN, Satyanarayan, Tay FR. Wettability of root canal sealers on intraradicular dentine treated with different irrigating solutions. J Dent 2013 Jun;41(6):556-560.
  19. Dai L, Khechen K, Khan S, Gillen B, Loushine BA, Wimmer CE, Gutmann JL, Pashley D, Tay FR. The effect of QMix, an experimental antibacterial root canal irrigant, on removal of canal wall smear layer and debris. J Endod 2011 Jan;37(1): 80-84.
  20. Singla MG, Garg A, Gupta S. MTAD in endodontics: an update review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Sep;112(3):e70-e76.
  21. Vemuri S, Kolanu SK, Varri S, Pabbati RK, Penumaka R, Bolla N. Effect of different final irrigating solutions on smear layer removal in apical third of root canal: a scanning electron microscope study. J Conserv Dent 2016 Jan-Feb;19(1):87-90.
  22. Paul ML, Mazumdar D, Niyogi A, Baranwal AK. Comparative evaluation of the efficacy of different irrigants including MTAD under SEM. J Conserv Dent 2013;16:336-341.
  23. Liolios E, Economides N, Parissis-Messimeris S, Boutsioukis A. The effectiveness of three irrigating solutions on root canal cleaning after hand and mechanical preparation. Int Endod J 1997 Jan;30(1):51-57.
  24. Kargula B, Özcanb M, Pekera S, Nakamoto T, Simmons WB, Falsterd AU. Evaluation of human enamel surfaces treated with theobromine: a pilot study. Oral Health Prev Dent 2012;10(3):275-282.
  25. Gold OG, Jordan HV, Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol 1973 Nov;18(11): 1357-1364.
  26. Loe H. The gingival index, the plaque index and the retention index systems. Journal of Periodontology.1967; 38(6 part II): 610-616.
  27. Mullally BH, James JA, Coulter WA, Linden GJ. The efficacy of a herbal-based toothpaste on the control of plaque and gingivitis. J Clin Periodontol 1995 Sep;22(9):686-689.
  28. Ozaki F, Pannuti CM, Imbronito AV, Pessotti W, Saraiva L, de Freitas NM, Ferrari G, Cabral VN. Efficacy of a herbal toothpaste on patients with established gingivitis—a randomized controlled trial. Braz Oral Res 2006 Jun;20(2):172-177.
  29. Jabbarifar SE, Tabibian SA, Poursina F. Effect of fluoride mouthrinse and toothpaste on number of streptococcal colony forming units of dental plaque. J Res Med Sci 2005;10(6): 363-367.
  30. Ooshima M, Osaka Y, Sasaki H, Osawa K, Yasuda H, Matsumura M, Sobue S, Matsumoto M. Caries inhibitory activity of cocao bean husk extract in in-vitro and animal experiments. Arch Oral Biol 2000 Aug;45(2):639-645.
  31. Sadhehpour A. A neural network analysis of theobromine vs fluoride on the enamel surface of human teeth. Dissertation Abstracts International, Vol. 68(07) (Suppl B), 2007; p. 150
  32. Babu NS, Vivek DK, Ambika G. Comparative evaluation of chlorhexidine mouthrinse versus cacao bean husk extract mouthrinse as antimicrobial agents in children. Eur Arch Paediatr Dent 2011 Oct 1;12(5):245-249.
  33. Dukle S, Patel A, Lakade L, Shah P, Lodaya R, Bha C. Comparison of chlorhexidine mouthrinse versus cacao bean husk extract mouthrinse with the addition of xylitol, as anti-plaque agents in children. Int J Curr Res 2017;9(7): 53680-53685.
  34. Eazhil R, Swaminathan SV, Gunaseelan M, Kannan GV, Alaqesan C. Impact of implant diameter and length on stress distribution in osseointegrated implants: a 3D FEA study. J Int Soc Prev Community Dent 2016 Nov-Dec;6(6):590-596.
  35. Abraham HM, Philip JM, Jain AR, Venkatakrishnan CJ. The effect of implant and abutment diameter on peri-implant bone stress: a 3D FEA analysis. J Oral Res Rev 2016 Oct;8(2): 49-52.
  36. Theobaldo JD, Aguiar FH, Pini NI, Lima DA, Liporoni PC, Catelan A. Effect of preheating and light-curing unit on physicochemical properties of a bulk fill composite. Clin Cosmet Investig Dent 2017 May;9:39-43.
  37. Tauböck TT, Tarle Z, Marovic D, Attin T. Preheating of highviscosity bulk fill resin composites: effects on shrinkage force and monomer conversion. J Dent 2015 Nov;43(11): 1358-1364.
  38. Dionysopoulos D, Tolidis D, Gerasimou P. The effect of composition, temperature and post-irradiation curing of bulk fill resin composites on polymerization efficiency. Mater Res 2016 Mar-Apr;19(2):466-473.
  39. Dionysopoulos D, Tolidis D, Gerasimou P, Koliniotou- Koumpia E. Effect of preheating on the film thickness of contemporary restorative materials. J Dent Sci 2014 Dec;9(4): 313-319.
  40. Zitzmann N, Walter C, Berglundh T. Atiologie, diagnostik und therapie der periimplantitis.eine ubersicht. Deutsche Zahnarztliche Zeitschrift 2006 Jan;61:642-649.
  41. Rams TE, Degener JE, van Winkelhoff AJ. Antibiotic resistance in human peri-implantitis microbiota. Clin Oral Implants Res 2013 Jan;25(1):82-90.
  42. Salonen MA, Oikarinen K, Virtanen K, Pernu H. Failures in the osseointegration of endosseous implants. Int J Oral Maxillofac Implants 1993;8(1):92-97.
  43. Zhang X, Sun Z, Yang Q. Application of Denaturing gradient gel electrophoresis to the analysis of bacterial communities associated with asymptomatic and symptomatic pericoronitis. J Oral Maxillofac Surg 2018 Mar;76(3):483-489.
  44. Suárez-López Del Amo F, Yu SH, Wang HL. Non-surgical therapy for peri-implant diseases: a systematic review. J Oral Maxillofac Res 2016 Sep;7(3):e13.
  45. Aberg CH, Kelk P, Johansson A. Aggregatibacter actinomycetemcomitans: virulence of its leukotoxin and association with aggressive periodontitis. Virulence 2015 Apr;6(3):188-195.
  46. Larsen OI, Enersen M, Kristoffersen AK, Wennerberg A, Bunæs DF, Lie SA, Leknes KN. Antimicrobial effects of three different treatment modalities on dental implant surfaces. J Oral Implantol 2017 Dec;43(6):429-436.
  47. Prathapachandran J, Suresh N. Management of periimplantitis. Dent Res J (Isfahan) 2012 Sep-Oct;9(5):516-521.
  48. Bhattacharyya M, Kumar AN, Kapuria S. Synthesis and characterization of Al/SiC and Ni/Al2O3 functionally graded materials. Mater Sci Eng A 2008 Jul;487(1-2):524-535.
  49. Chenglin C, Jingchuan Z, Zhongda Y, Shidong W. Hydroxyapatite-Ti functionally graded biomaterial fabricated by powder metallurgy. Mater Sci Eng A 1999 Nov;271(1):95-100.
  50. Oleiwi JK, Anaee RA, Muhsin SA. Fabrication, characterization and physical properties of functionally graded Ti/HAP bioimplants. Wulfenia J 2015 Jul;22(7):336-348.
  51. Ogawa T, Watanabe Y, Sato H, Kim IS, Fukui Y. Theoretical study on fabrication of functionally graded material with density gradient by a centrifugal solid-particle method. Compos Part A Appl S 2006 Dec;37(12):2194-2200.
  52. Yang ZM, Tian F, Zhang LM. Theoretical study on two sedimentation processes used to form functionally graded materials. J Mater Sci Lett 2003 May;22(10):739-741.
  53. Yang ZM, Gong DR, Zhang LM. Design of co-sedimentation experiments used to fabricate functionally graded materials with a continuous change of composition. J Wuhan Univ Technol Mater Sci Ed 2004 Jan;19(2):11-13.
  54. Jin G, Takeuchi M, Honda S, Nishikawa T, Awaji H. Properties of multilayered mullite/Mo functionally graded materials fabricated by powder metallurgy processing. Mater Chem Physics 2005 Feb;89(2):238-243.
  55. Jamaludin SN, Basri S, Hussain A, Al-Othmany DS, Mustapha F, Nuruzzaman DM. Three-dimensional finite element modeling of thermomechanical problems in functionally graded hydroxyapatite/titanium plate. Math Probl Eng 2014 Apr;2014:371462.
  56. Halpern LR, Dodson TB. Does prophylactic administration of systemic antibiotics prevent postoperative inflammatory complications after third molar surgery? J Oral Maxillofac Surg 2007 Feb;65(2):177-185.
  57. Lopez-Cedrun JL, Pijoan JI, Fernandez S, Santamaria J, Hernandez G. Efficacy of amoxycillin treatment in preventing postoperative complications in patients undergoing third molar surgery: a prospective, randomized, double-blind controlled study. J Oral Maxillofac Surg 2011 Jun;69(6):e5-e14.
  58. Arteagoitia MI, Barbier L, Santamaria J, Santamaria G, Ramos E. Efficacy of amoxicillin and amoxicillin/clavulanic acid in the prevention of infection and dry socket after third molar extraction. A systematic review and meta-analysis. Med Oral Patol Oral Cir Bucal 2016 Jul;21(4):e494-e504.
  59. Piltz JR, Starita RJ. The use of subconjunctivally administered tissue plasminogen activator after trabeculectomy. Ophthalmic Surg 1994 Jan;25(1):51-53.
  60. Por YM, Tan YL, Mehta JS, Tan DT. Intracameral fibrin tissue sealant as an adjunct in tectonic lamellar keratoplasty for large corneal perforations. Cornea 2009 May;28(4):451-455.
  61. Sakarya Y, Sakarya R, Yildirim A. Sutureless amniotic membrane fixation with fibrin glue in symptomatic bullous keratopathy with poor visual potential. Eur J Ophthalmol 2010 Jan-Feb;20(1):249; author reply 249-250.
  62. Lee GA, Holcombe DJ. Surgical revision of dysfunctional filtration blebs with bleb preservation sliding conjunctival flap and fibrin glue. Eye (Lond) 2010 Jun;24(6):947-953.
  63. Kahook MY, Noecker RJ. Fibrin glue-assisted glaucoma drainage device surgery. Br J Ophthalmol 2006 Dec;90(12):1486-1489.
  64. Valimaki J. Fibrin glue for preventing immediate postoperative hypotony following glaucoma drainage implant surgery. Acta Ophthalmol Scand 2006 Jun;84(3):372-374.
  65. Malhotra C, Jain AK. Human amniotic membrane transplantation: different modalities of its use in ophthalmology. World J Transplant 2014 Jun;4(2):111-121.
  66. Dua HS, Gomes JA, King AJ, Maharajan VS. The amniotic membrane in ophthalmology. Surv Ophthalmol 2004 Jan-Feb;49(1):51-77.
  67. Shimmura S, Shimazaki J, Ohashi Y, Tsubota K. Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea 2001 May;20(4):408-413.
  68. Yazdani S, Mahboobipour H, Pakravan M, Doozandeh A, Ghahari E. Adjunctive mitomycin c or amniotic membrane transplantation for Ahmed glaucoma valve implantation: a randomized clinical trial. J Glaucoma 2016 May;25(5):415-421.
  69. Sheha H, Kheirkhah A, Taha H. Amniotic membrane transplantation in trabeculectomy with mitomycin C for refractory glaucoma. J Glaucoma 2008 Jun-Jul;17(4):303-307.
  70. Khairy HA, Elsawy MF. Trabeculectomy with mitomycin-C versus trabeculectomy with amniotic membrane transplant: a medium-term randomized controlled trial. J Glaucoma 2015 Sep;24(7):556-559.
  71. Rauscher FM, Barton K, Budenz DL, Feuer WJ, Tseng SC. Long-term outcomes of amniotic membrane transplantation for repair of leaking glaucoma filtering blebs. Am J Ophthalmol 2007 Jun;143(6):1052-1054.
  72. Sethi P, Patel RN, Goldhardt R, Ayyala RS. Conjunctival advancement with subconjunctival amniotic membrane draping technique for leaking cystic blebs. J Glaucoma 2016 Feb;25(2):188-192.
  73. Kitagawa K, Yanagisawa S, Watanabe K, Yunoki T, Hayashi A, Okabe M, Nikaido T. A hyperdry amniotic membrane patch using a tissue adhesive for corneal perforations and bleb leaks. Am J Ophthalmol 2009 Sep;148(3):383-389.
  74. Nagai-Kusuhara A, Nakamura M, Fujioka M, Negi A. Longterm results of amniotic membrane transplantation-assisted bleb revision for leaking blebs. Graefes Arch Clin Exp Ophthalmol 2008 Apr;246(4):567-571.
  75. Rai P, Lauande-Pimentel R, Barton K. Amniotic membrane as an adjunct to donor sclera in the repair of exposed glaucoma drainage devices. Am J Ophthalmol 2005 Dec;140(6): 1148-1152.
  76. Ainsworth G, Rotchford A, Dua HS, King AJ. A novel use of amniotic membrane in the management of tube exposure following glaucoma tube shunt surgery. Br J Ophthalmol 2006 Apr;90(4):417-419.
  77. Papadaki TG, Siganos CS, Zacharopoulos IP, Panteleontidis V, Charissis SK. Human amniotic membrane transplantation for tube exposure after glaucoma drainage device implantation. J Glaucoma 2007 Jan;16(1):171-172.
  78. Mulder EJ, Leiblum DM, Visser GH. Fetal breathing movements in late diabetic pregnancy: relationship to fetal heart rate patterns and Braxton Hicks’ contractions. Early Hum Dev 1995 Nov;43(3):225-232.
  79. Schulte FJ, Michaelis R, Nolte R, Albert G, Parl U, Lasson U. Brain and behavioral maturation in newborn infants of diabetic mothers. I. Nerve conduction and EEG patterns. Neuropadiatrie 1969 Jan-Jul;1(1):24-35.
  80. Devoe LD, Youssef AA, Castillo RA, Croom CS. Fetal biophysical activities in third-trimester pregnancies complicated by diabetes mellitus. Am J Obstet Gynecol 1994 Aug;171(2):298-303.
  81. Dierker LJ Jr, Pillay S, Sorokin Y, Rosen MG. The change in fetal activity periods in diabetic and nondiabetic pregnancies. Am J Obstet Gynecol 1982 May;143(2):181-185.
  82. Doherty NN, Hepper PG. Habituation in fetuses of diabetic mothers. Early Hum Dev 2000 Aug;59(2):85-93.
  83. Kainer F, Prechtl HF, Engele H, Einspieler C. Assessment of the quality of general movements in fetuses and infants of women with type-1 diabetes mellitus. Early Hum Dev 1997 Nov;50(1):13-25.
  84. Mulder EJ, O'Brien MJ, Lems YL, Visser GH, Prechtl HF. Body and breathing movements in near-term fetuses and newborn infants of type-1 diabetic women. Early Hum Dev 1990 Nov;24(2):131-152.
  85. Mulder EJ, Visser GH. Growth and motor development in fetuses of women with type-1 diabetes. I. Early growth patterns. Early Hum Dev 1991 May;25(2):91-106.
  86. Mulder EJ, Visser GH. Growth and motor development in fetuses of women with type-1 diabetes. II. Emergence of specific movement patterns. Early Hum Dev 1991 May;25(2):107-115.
  87. Mulder EJ, Visser GH. Impact of early growth delay on subsequent fetal growth and functional development: a study on diabetic pregnancy. Early Hum Dev 1992 Dec;31(2):91-95.
  88. Mulder EJ, Visser GH, Bekedam DJ, Prechtl HF. Emergence of behavioral states in fetuses of type-1 diabetic women. Early Hum Dev 1987 Jul;15(4):231-251.
  89. Mulder EJ, Visser GH, Morssink LP, de Vries JI. Growth and motor development in fetuses of women with type-1 diabetes. III. First trimester quantity of fetal movement patterns. Early Hum Dev 1991 May;25(2):117-133.
  90. Aberg A, Westbom L, Kallen B. Congenital malformations among infants whose mothers had gestational diabetes or preexisting diabetes. Early Hum Dev 2001 Mar;61(2):85-95.
  91. Deregnier RA, Nelson CA, Thomas KM, Wewerka S, Georgieff MK. Neurophysiologic evaluation of auditory recognition memory in healthy newborn infants and infants of diabetic mothers. J Pediatr 2000 Dec;137(6):777-784.
  92. Nelson CA, Wewerka S, Thomas KM, Tribby-Walbridge S, deRegnier R, Georgieff M. Neurocognitive sequelae of infants of diabetic mothers. Behav Neurosci 2000 Oct;114(5):950-956.
  93. Reece EA, Homko CJ. Infant of the diabetic mother. Semin Perinatol 1994 Oct;18(5):459-469.
  94. Reece EA, Homko CJ. Why do diabetic women deliver malformed infants? Clin Obstet Gynecol 2000 Mar;43(1):32-45.
  95. Rizzo T, Metzger BE, Burns WJ, Burns K. Correlations between antepartum maternal metabolism and intelligence of offspring. N Engl J Med 1991 Sep;325(13):911-916.
  96. Rizzo TA, Metzger BE, Dooley SL, Cho NH. Early malnutrition and child neurobehavioral development: insights from the study of children of diabetic mothers. Child Dev 1997 Feb;68(1):26-38.
  97. Schwartz R, Teramo KA. Effects of diabetic pregnancy on the fetus and newborn. Semin Perinatol 2000 Apr;24(2):120-135.
  98. Vaarasmaki MS, Hartikainen A, Anttila M, Pramila S, Koivisto M. Factors predicting peri- and neonatal outcome in diabetic pregnancy. Early Hum Dev 2000 Jul;59(1):61-70.
  99. Robertson SS, Dierker LJ. Fetal cyclic motor activity in diabetic pregnancies: sensitivity to maternal blood glucose. Dev Psychobiol 2003 Jan;42(1):9-16.
  100. Accardo PJ, Blondis TA, Whitman BY. Disorders of attention and activity level in a referral population. Pediatrics 1990 Mar;85(3 Pt 2):426-431.
  101. Ornoy A, Uriel L, Tennenbaum A. Inattention, hyperactivity and speech delay at 2-4 years of age as a predictor for ADDADHD syndrome. Isr J Psychiatry Relat Sci 1993;30(3):155-163.
  102. Smyth TR. Impaired motor skill (clumsiness) in otherwise normal children: a review. Child Care Health Dev 1992 Sep-Oct;18(5):283-300.
  103. Petersen MB, Pedersen SA, Greisen G, Pedersen JF, Mølsted- Pedersen L. Early growth delay in diabetic pregnancy: relation to psychomotor development at age 4. Br Med J (Clin Res Ed) 1988 Feb;296(6622):598-600.
  104. Sells CJ, Robinson NM, Brown Z, Knopp RH. Long-term developmental follow-up of infants of diabetic mothers. J Pediatr 1994 Jul;125(1):S9-S17.
  105. Vladareanu R, Lebit D, Constantinescu S. Ultrasound assessment of fetal neurobehaviour in high risk pregnancies. DSJUOG 2012 Apr-Jun;6(2):132-147.
  106. Talic A, Kurjak A, Ahmed B, Stanojevic M, Predojevic M, Kadic AS, Di Renzo GC. The potential of 4D sonography in the assessment of fetal behavior in high-risk pregnancies. J Matern Fetal Neonatal Med 2011 Jul;24(7):948-954.
  107. Bekedam DJ, Visser GH, de Vries JJ, Prechtl HF. Motor behaviour in the growth retarded fetus. Early Hum Dev 1985 Nov;12(2):155-165.
  108. Cioni G, Prechtl HF. Preterm and early postterm motor behaviour in low-risk premature infants. Early Hum Dev 1990 Sep;23(3):159-191.
  109. Seme-Ciglenecki P. Predictive value of assessment of general movements for neurological development of highrisk preterm infants: comparative study. Croat Med J 2003 Dec;44(6):721-727.
  110. Abo-Yaqoub S, Kurjak A, Mohammed AB, Shadad A, Abdel-Maaboud M. The role of 4-D ultrasonography in prenatal assessment of fetal neurobehaviour and prediction of neurological outcome. J Matern Fetal Neonatal Med 2012 Mar;25(3):231-236.
  111. Athanasiadis AP, Mikos T, Tambakoudis GP, Theodoridis TD, Papastergiou M, Assimakopoulos E, Tarlatzis BC. Neurodevelopmental fetal assessment using KANET scoring system in low and high-risk pregnancies. J Matern Fetal Neonatal Med 2013 Mar;26(4):363-368.
  112. Fauser BC, Tarlatzis BC, Rebar RW, Legro RS, Balen AH, Lobo R, Carmina E, Chang J, Yildiz BO, Laven JS, et al. Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil Steril 2012 Jan;97(1):28-38.e25
  113. Sirmans SM, Pate KA. Epidemiology, diagnosis and management of polycystic ovary syndrome. Clin Epidemiol 2014; 6:1-13.
  114. Fauser BC, Diedrich K, Bouchard P, Domínguez F, Matzuk M, Franks S, Hamamah S, Simón C, Devroey P, Ezcurra D, et al. Contemporary genetic technologies and female reproduction. Human Reproduction Update 2011 Nov;17(6):829-847.
  115. Balen A, Conway G, Kaltsas G. Polycystic ovary syndrome: the spectrum of the disorder in 1741 patients. Hum Reprod 1995 Aug;10(8):2107-2111.
  116. Azziz R, Sanchez L, Knochenhauer ES, Moran C, Lazenby J, Stephens KC, Taylor K, Boots LR. Androgen excess in women: experience with over 1000 consecutive patients. J Clin Endocrinol Metab 2004 Feb;89(2):453-462.
  117. Wild S, Pierpoint T, McKeigue P, Jacobs H. Cardiovascular disease in women with polycystic ovary syndrome at longterm follow-up: a retrospective cohort study. Clin Endocrinol (Oxf) 2000 May;52(5):595-600.
  118. Hollinrake E, Abreu A, Maifeld M, Van Voorhis BJ, Dokras A. Increased risk of depressive disorders in women with polycystic ovary syndrome. Fertil Steril 2007 Jun;87(6): 1369-1376.
  119. Zawadski JK, Dunaif A. Diagnostic criteria for polycystic ovary syndrome. In: Givens JHF, Merriman G, editors. The polycystic ovary syndrome. Cambridge, MA: Blackwell Scientific; 1992. p. 377-384.
  120. Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril 2004 Jan;81(1):19-25.
  121. Azziz R, Carmina E, DeWailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Futterweit W, Janssen OE, Legro RS, Norman RJ, Taylor AE, et al. Position statement: criteria for defining polycystic ovary syndrome as a predominantly hyperandrogenic syndrome: an Androgen Excess Society guideline. J Clin Endocrinol Metab 2006 Nov;91(11):4237-4245.
  122. Lujan ME, Jarrett BY, Brooks ED, Reines JK, Peppin AK, Muhn N, Haider E, Pierson RA, Chizen DR. Updated ultrasound criteria for polycystic ovary syndrome: reliable thresholds for elevated follicle population and ovarian volume. Hum Reprod 2013 May;28(5):1361-1368.
  123. Badawy A, Elnashar A. Treatment options for polycystic ovary syndrome. Int J Womens Health 2011 Feb;3:25-35.
  124. ACOG Committee on Practice Bulletins—Gynecology. ACOG Practice Bulletin No 108: Polycystic ovary syndrome. Obstet Gynecol 2009 Oct;114(4):936-949.
  125. Thessaloniki ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group Consensus on infertility treatment related to polycystic ovary syndrome. Fertil Steril 2008 Mar;89(3): 505-522.
  126. Balen AH. Ovulation induction in the management of anovulatory polycystic ovary syndrome. Mol Cell Endocrinol 2013 Jul;373(1-2):77-82.
  127. Berridge DL, Winter TC. Saline infusion sonohysterography: technique, indications, and imaging findings. J Ultrasound Med 2004 Jan;23(1):97-112.
  128. Allison SJ, Horrow MM, Kim HY, Lev-Toaff AS. Salineinfused sonohysterography: tips for achieving greater success. Radiographics 2011 Nov-Dec;31(7):1991-2004.
  129. O'Neill MJ. Sonohysterography. Radiol Clin North Am 2003 Jul;41(4):781-797.
  130. Bonnamy L, Marret H, Perrotin F, Body G, Berger C, Lansac J. Sonohysterography: a prospective survey of results and complications in 81 patients. Eur J Obstet Gynecol Reprod Biol 2002 Apr;102(1):42-47.
  131. Lindheim SR, Adsuar N, Kushner DM, Pritts EA, Olive DL. Sonohysterography: a valuable tool in evaluating the female pelvis. Obstet Gynecol Surv 2003 Nov;58(11):770-784.
  132. Lindheim SR, Sprague C, Winter TC. Hysterosalpingography and sonohysterography: lessons in technique. AJR Am J Roentgenol 2006 Jan;186(1):24-29.
  133. Jeanty P, Besnard S, Arnold A, Turner C, Crum P. Air-contrast sonohysterography as a first step assessment of tubal patency. J Ultrasound Med 2000 Aug;19(8):519-527.
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