Citation Information :
Ranju MB, Girish MS, Prashanth S. Comparative Evaluation of Antimicrobial Efficacy of Theobromine Toothpaste and a Low-fluoridated Toothpaste against Plaque Streptococcus mutans in Children. World J Dent 2018; 9 (3):215-219.
Introduction: Cocoa bean extracts contain certain anticariogenic substances and studies have proven that it has a role in preventing dental caries. Theobromine, a white crystalline powder, is an alkaloid, readily available in cocoa and chocolate has been considered responsible for its anticariogenic activity.
Aim: The aim of the present study was to evaluate the in vivo anticaries activity of theobromine toothpaste and a commercially available low-fluoridated toothpaste against plaque Streptococcus mutans in children of age group 6 to 9 years and to evaluate the efficacy of both the toothpastes on supragingival plaque.
Materials and methods: A total of 14 subjects were selected and randomly divided into experimental and control groups (n=7) in which theobromine toothpaste and a low-fluoridated toothpaste were provided. The subjects were instructed to carry out toothbrushing twice daily for 1 minute and the plaque samples were collected at baseline and after 21 days of intervention. Microbial analysis was done using selective media, mitis salivarius bacitracin (MSB) agar. The plaque index (Turesky modification of Quigley–Hein index) and gingival index (Loe and Silness) were recorded at baseline and after 21 days. The values were statistically analyzed using paired and unpaired t test.
Results: There was a significant reduction in the S. mutans count and plaque scores from baseline to postintervention in both the groups (p = 0.001). When both the groups were compared, there was no significant difference in the mean S. mutans count and plaque scores (p = 0.254).
Conclusion: Theobromine toothpaste is highly effective in reducing S. mutans count and plaque formation in children.
Clinical significance: In the future, theobromine toothpaste will prove to be beneficial as a safe anticariogenic agent, which could open a new era of approach to caries prevention.
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