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VOLUME 8 , ISSUE 1 ( January-February, 2017 ) > List of Articles

RESEARCH ARTICLE

Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres

Ravindra Kotian, P Prasad Rao, Prashanthi Madhyastha, KL Shobha, BS Satish Rao

Citation Information : Kotian R, Rao PP, Madhyastha P, Shobha K, Rao BS. Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World J Dent 2017; 8 (1):28-36.

DOI: 10.5005/jp-journals-10015-1406

Published Online: 00-02-2017

Copyright Statement:  Copyright © 2017; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim

This study was performed to evaluate the biocompatibility of pure titanium and Ti-6Al-4V metals coated with hydroxyapatite (HA) by plasma spray using different plasma gas atmospheres.

Materials and methods

The cell viabilities for each HA-coated sample in an atmosphere of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen were studied using MTT assay and platelet adhesion test.

Results

The mean cell viabilities by MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] assay of samples coated with HA in argon—hydrogen plasma atmosphere showed maximum cell viability at different time intervals compared with other coating atmospheres of argon—hydrogen, nitrogen, and nitrogen—hydrogen. A statistically significant value of cell viability (p < 0.001) was observed between and within the groups of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen plasma gas atmosphere. The platelet adhesion study showed agglomerates of platelet cells in some isolated regions of HA for all atmospheres.

Significance

The results obtained in this study can serve as a guide for the development of new Ti-based HA-coated implants in different plasma gas atmospheres.

How to cite this article

Kotian R, Rao PP, Madhyastha P, Shobha KL, Rao BSS, Ginjupalli K. Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World J Dent 2017;8(1):28-36.


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  1. Development of endosseous implants on the base titanium alloys with improved functionality. In: Donath K, editor. The minerals, metals and materials society, Titanium 92 Science and Technology, San Diego, CA; 1992. p. 2757-2764.
  2. Influence of spray parameters on the microstructure and mechanical properties of gas-tunnel plasma sprayed hydroxyapatite coatings. Mater Sci Eng 2007;B139:209-215.
  3. Changes in nonosseous tissue adjacent to titanium implants. J Biomed Mater Res 1973 Nov;7(6):555-572.
  4. Characterization of porous hydroxyapatite. J Mater Sci Mater Med 1999 Mar;10(3):135-145.
  5. Hydroxyapatite coating. Ann NY Acad Sci 1998;523:72-80.
  6. Hydroxyapatite coating by dipping method and bone bonding strength. J Mater Sci Mater Med 1996;7:355-357.
  7. Structural analysis of hydroxyapatite coating on titanium. J Am Ceramic Soc 1984;6B:381-384.
  8. Auger electron spectroscopic analysis of hydroxyapatite coating on titanium. J Am Ceramic Soc 1984;63:381-384.
  9. Post-deposition heat treatments for ion beam sputter deposited calcium phosphate coating. Biomaterials 1994 Apr;15(5):337-341.
  10. Thin hydroxyapatite coating produced by the ion beam dynamic mixing method. Biomaterials 1994 Jun;15(7):529-535.
  11. Thermal spraying for bio ceramic applications. Mater Forum 1990;14: 161-173.
  12. Flame sprayed bio ceramics. Am Ceramic Soc Bull 1975;52:898
  13. Raman microprobe investigation of the calcium phosphate phases of three commercially available plasma-flame sprayed hydroxyapatite coated dental implants. J Mater Sci Mater Med 1992;3:397-401.
  14. Characterization of high velocity oxy-fuel combustion sprayed hydroxyapatite. Biomaterials 1995 Feb;16(3):229-237.
  15. Structure and properties of hydroxyapatite bioactive glass composites plasma sprayed on Ti-6Al-4V. J Mater Sci Mater Med 1994;3:279-283.
  16. Influence of spraying conditions on thermal and velocity properties of plasma sprayed hydroxyapatite. Mater Sci Eng 2007;C27:340-344.
  17. Adhesion properties of plasma sprayed hydroxyapatite coatings for orthopedic prosthesis. Biomed Mater Eng 1993 Fall;3(3):127-136.
  18. Biomaterials in orthopaedics. Basel: Marcel Dekker; 2004. p. 401-423.
  19. Plasma sprayed hydroxyapatite coatings on titanium substrates. Part 1: Mechanical properties and residual stress levels. Biomaterials 1998 Nov;19(22):2015-2029.
  20. Phase, structural and microstructural investigations of plasma sprayed hydroxyapatite. Mater Sci Eng 2003;360A:70-84.
  21. Effect of titanium and titania on chemical characteristics of hydroxyapatite plasma-sprayed into water. Mater Sci Eng 2006;26C:28-33.
  22. Plasma sprayed hydroxyapatite coatings on titanium substrates. Part 2: Optimisation of coating properties. Biomaterials 1998 Nov;19(22):2031-2043.
  23. Effect of gun current on the microstructure and crystallinity of plasma sprayed hydroxyapatite coatings. Appl Surf Sci 2007 Jun;253(17):7136-7142.
  24. Influence of gas rate on the microstructure and mechanical properties of hydroxyapatite coatings fabricated by gas tunnel type plasma spray. Surf Coat Technol 2006;201:2560-2566.
  25. Structure, mechanical performance and electrochemical characterization of plasma sprayed SiO2/Ti-reinforced hydroxyapatite biomedical coatings. Appl Surf Sci 2008;255:3426-3433.
  26. Influence of processing parameters and heat treatment on the phase composition and microstructure of plasma sprayed hydroxyapatite coatings. Trans Nonferrous Met Soc China 2009;19:463-469.
  27. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983 Dec 16;65(1-2):55–63.
  28. In vitro study of platelet adhesion on medical nickel-free stainless steel surface. Mater Lett 2005;59:1785-1789.
  29. A historical evaluation of TiO2-gritblasted and Ca-P magnetron sputter coated implants placed into the trabecular bone of the goat: part 2. Clin Oral Implants Res 2000 Aug;11(4):314-324.
  30. Initial interfacial healing events around calcium phosphate (Ca-P) coated oral implants. Clin Oral Implants Res 1997 Oct;8(5):393-400.
  31. Biological performance of biomimetic calcium phosphate coating of titanium implants in the dog mandible. J Biomed Mater Res A 2003 Feb 1;64(2):225-234.
  32. Platelet interactions with titanium: modulation of platelet activity by surface topography. Biomaterials 2001 Oct;22(19):2671-2682.
  33. Mechanical circulatory support-a long and winding road. Science 2002 Feb 8;295(5557):998-999.
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