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VOLUME 7 , ISSUE 2 ( April-June, 2016 ) > List of Articles

RESEARCH ARTICLE

Tissue Preservation with Natural Fixatives: An Immunohistochemical Evaluation

Barnali Majumdar

Citation Information : Majumdar B. Tissue Preservation with Natural Fixatives: An Immunohistochemical Evaluation. World J Dent 2016; 7 (2):87-91.

DOI: 10.5005/jp-journals-10015-1371

Published Online: 00-06-2016

Copyright Statement:  Copyright © 2016; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background

The search for formalin substitute has been enduring from the time it was proven as a carcinogen. The natural sweetener, jaggery, as a tissue fixative has already been established and its compatibility with special stains demonstrated in our previous study. Hence, in the present study, the efficacy of jaggery, along with honey, was further evaluated using immunohistochemical procedures.

Materials and methods

A cross-sectional study with 30 samples of commercially available fresh goat oral mucosa, fixed with 10% buffered formalin (positive control), 20% honey, and 30% jaggery solution was conducted. After 24 hours of fixation, tissue sections were subjected to immunohistochemical stains pan-cytokeratin and desmin. Following which blinded evaluation was done by two examiners. Statistical analysis was accomplished using Kruskal—Wallis analysis of variance, Mann—Whitney U test, chi-square test, and Kappa statistics.

Results

The pan-cytokeratin staining intensity of jaggeryfixed tissues (2.67 ± 0.488) was observed to be more intense than honey (1.60 ± 0.507, p < 0.001) and formalin (2.13 ± 0.352, p = 0.003)-fixed tissues. Jaggery (2.33 ± 0.617) and formalin (2.40 ± 0.632) showed comparable results with respect to desmin.

Conclusion

Based on the findings, the fixative properties of jaggery surpassed honey and was found to be on par with formalin.

How to cite this article

Majumdar B, Rao RS, Patil S. Tissue Preservation with Natural Fixatives: An Immunohistochemical Evaluation. World J Dent 2016;7(2):87-91.


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