World Journal of Dentistry

Register      Login

VOLUME 6 , ISSUE 3 ( July-September, 2015 ) > List of Articles


Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth

S Srilatha, Zeeshan Hasanali Ladhani, Sayli Dargad, Vighnesh Dixit

Citation Information : Srilatha S, Ladhani ZH, Dargad S, Dixit V. Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth. World J Dent 2015; 6 (3):174-177.

DOI: 10.5005/jp-journals-10015-1337

Published Online: 01-04-2017

Copyright Statement:  Copyright © 2015; The Author(s).



To evaluate microhardness of enamel surface after microabrasion of enamel using casein phosphopeptideamorphous calcium phosphate (CPP-ACP) and casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF).

Materials and methods

Ten freshly extracted anterior teeth were selected. Teeth were treated with abrasive slurry. This prepared slurry was applied to the labial aspects of teeth, using a rotating rubber cup, for 50 seconds. Each tooth was divided into four parts and treated accordingly with the topical application of the demineralizing agent stored in artificial saliva.

The teeth were divided into four groups, as follows:

– Group A: Control group

– Group B: Microabrasion done but not treated

– Group C: Microabrasion done and CPP-ACP paste applied

– Group D: Microabrasion done and CPP-ACPF (i.e. with fluoride) applied.

The microhardness was evaluated using the Vicker's microhardness test.


Microhardness values were highest for the control group followed by group D, group C and finally group B. Microabraded group of teeth with no topical application showed least microhardness values of all.


The CPP-ACPF increased the microabrasion significantly as compared to CPP-ACP and microabrasion group.

How to cite this article

Ladhani ZH, Dargad S, Dixit V, Srilatha S, Hegde V. Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth. World J Dent 2015;6(3):174-177.

PDF Share
  1. Enamel Microabrasion: observations after 10 years. J Am Dent Assoc 1997;128:45-50.
  2. A Technique for removal of fluorosis stains. J Am Dent Assoc 1984;109:63-64.
  3. Enamel color modification by controlled hydrochloric acid-pumice abrasion: Part 1. Technique and examples. Quintessence Int 1986;17:81-87.
  4. Enamel microabrasion for removal of superficial dysmineralization and decalcification defects. J Am Dent Assoc 1990;120:411-415.
  5. Textbook-enamel microabrasion. The technique 1991;395-400.
  6. In esthetic dentistry and ceramic restorations. London: Martin Dunitz. Treatment of tooth discolouration; 1999. p. 83-116.
  7. The effects of microabrasion on demineralization inhibition of enamel surfaces. Quintessence Int 1997;28:463-466.
  8. Presence or absence of plaque on subgingival restorations. Scand J Dent Res 1975;83:193-201.
  9. Remineralisation, the natural caries repair process—the need for new approaches. Adv Dent Res 2009; 21(1):4-7.
  10. Effects of continuous-wave COz laser on the ultrastructure of human dental enamel. Arch Oral Biol 1989;34:551-562.
  11. Effect of the fluoride gels and varnishes comparing to CPP-ACP complex on human enamel demineralization/ remineralization. Acta stomatol Croat 2013;47:99-110.
  12. The effect of fluoridated dentifrice formulations on enamel remineralisation and microhardness after in vitro demineralization. Acta Stomatol Croat 2011;45:159-165.
  13. Remineralization potential of fluoride and amorphous calcium phosphatecasein phosphopeptide on enamel lesions: an in vitro comparative evaluation. J Conserv Dent 2010;13:42-46.
  14. Timing of flouride toothpaste use and enamel dentin demineralization. Braz Oral Res 2011;25:1-6.
  15. Effect of fluoridated dentifrices on surface microhardness of the enamel of deciduous teeth. Dent Res 2011;8: 113-117.
  16. The effect of high calcium milk and casein phosphopeptide-amorphous calcium phosphate on enamel erosion caused by cholinated water. Southeast Asian J Trop Med Public Health 2010;41:1494-1499.
  17. Preventive dentistry for the general dental practitioner. Aust Dent J 2000;45:76-82.
  18. Anticariogenic casein phosphopeptides. Prot Peptide Lett 1999;6:295-303.
  19. Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques. Arch Oral Biol 2000;45:569-575.
  20. Remineralization of enamel subsurface lesions by casein phosphopeptide-stabilized calcium phosphate solutions. J Dent Res 1997;76(9):1587-1595.
  21. The role of phosphopeptides in caries prevention. Dent Perspectives 1999;3:67.
  22. Remineralization of early enamel lesions using casein phosphopeptide amorphous calcium phosphate: an ex-vivo study. Contemp Clini Dent 2010;1:210-213.
  23. Effect of CPP-ACP paste on tooth mineralization on FE-SEM study. J Oral Sci 2007;49:115-120.
  24. The effects of three different pastes on enamel caries formation and lesion depth progression: an in vitro study. J Oral Health Comm Dent 2007;1:1-6.
  25. The comparison of enamel hardness between fluoride and theobromine application. Int J Contemp Dent Med Rev 2014;1-4.
  26. Fluoride and casein phosphopeptideamorphous calcium phosphate. J Dent Res 2008;87(4):344-348.
  27. In vitro remineralization efficacy of NaF systems containing unique forms of calcium. Amer J Dent 2009;22:185-188.
  28. Protective effect of three different fluorides pretreatment on artificially induced dental erosion in primary and permanent teeth. J Amer Sci 2010;6:442-450.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.