World Journal of Dentistry

Register      Login

VOLUME 3 , ISSUE 4 ( October-December, 2012 ) > List of Articles


Antimicrobial Effects of the Combination of Chlorhexidine and Fluoride Varnish in Young Adult Population

BV Sreenivasa Murthy, Swaroop Hegde, KH Sumeera, Sylvia Mathew, John V George

Citation Information : Murthy BS, Hegde S, Sumeera K, Mathew S, George JV. Antimicrobial Effects of the Combination of Chlorhexidine and Fluoride Varnish in Young Adult Population. World J Dent 2012; 3 (4):290-293.

DOI: 10.5005/jp-journals-10015-1175

Published Online: 01-12-2012

Copyright Statement:  Copyright © 2012; The Author(s).



(1) Use of a cariogram to evaluate the caries risk assessment in the students. (2) To evaluate the effectiveness of chlorhexidine and fluoride varnish applications on Streptococcus mutans at a 3-month follow-up.

Materials and methods

All the students of first year BDS of MS Ramaiah Dental College were included in this study. Caries risk assessment was done using a Cariogram and the students were divided into moderate and low risk groups. Streptococcus mutans count was assessed at baseline for both the groups. Chlorhexidine and fluoride varnish application was done in the moderate risk group at an interval of 1 week. After a period of 3 months, the Streptococcus mutans count was determined for both the groups and the results statistically analyzed.


A significant reduction in the Streptococcus mutans count in the moderate risk group was seen at a 3 month follow-up.


The combined use of both chlorhexidine and fluoride varnish applications reduced the oral Streptococcus mutans count to significantly low levels in the study group at the 3-month follow-up.

How to cite this article

Sumeera KH, Mathew S, Hegde S, Murthy BVS, George JV. Antimicrobial Effects of the Combination of Chlorhexidine and Fluoride Varnish in Young Adult Population. World J Dent 2012;3(4):290-293.

PDF Share
  1. Assessing patients caries risk. J Am Dent Assoc 2006;137(9):123-19.
  2. Anderson M, How to integrate CAMBRA into private practice. J Calif Dent Assoc 2007;35(11):778-85.
  3. Evaluation of a computer-based caries risk assessment program in an elderly group of individuals. Acta Odontol Scand 2003;61(3):164-71.
  4. Evaluation of a computer program for caries risk assessment in schoolchildren. Caries Res 2002;36:327-40.
  5. Effect of sodium fluoride varnish on the progress of initial caries lesions. Scand J Dent Res 1983;91:96-98.
  6. The effect of fluoride in saliva on remineralization of dental enamel in humans. Caries Res 1981;15:341-45.
  7. A simultaneous reduction of caries and gingivitis in a group of school children receiving chlorhexidine-fluoride applications. Results after 2 years. Caries Res 1978;12:290-98.
  8. The use of fluoride and chlorhexidine for the prevention of radiation caries. J Am Dent Assoc 1982;104:164-70.
  9. A review of the literature on use of chlorhexidine in dentistry. J Amer Dent Assoc 1986;112:863-69.
  10. Caries-preventive effect of chlorhexidine gel applications among high-risk children. Caries Res 2006;40:514-21.
  11. Effect of chlorhexidine-thymol varnish on caries lesion development in first permanent molars. J Clin Dent 2008;19:18-21.
  12. Effect of chlorhexidine on the relative proportions of Streptococcus mutans and Streptococcus sanguis in hamster plaque. Scan J Dent Res 1979;87:288-95.
  13. Effect of caries preventive measures in children highly infected with the bacterium Streptococcus mutans. Arch Oral Biol 1982;27:861-68.
  14. Effect of tooth brushing with chlorhexidine gel on salivary microflora, oral hygiene and caries. Scan J Dent Res 1976;84:308-19.
  15. Development of sustained-release antimicrobial dental varnishes effective against Streptococcus mutans in vitro. J Dent Res 1985;64:1356-60.
  16. Caries Res 1991;25(4):292-95.
  17. Effect of single and repeated application of chlorhexidine varnish on mutans Streptococci in plaque from fissures of premolar and molar teeth. Caries Res 1993;27(4):303-06.
  18. Effect of Cervitec on mutans Streptococci in plaque and on caries formation on occlusal fissures of erupting permanent molars. Caries Res 2002;36(5):373-76.
  19. Effects of combined application of antimicrobial and fluoride varnishes in orthodontic patients. Am J Orthod Dentofacial Orthop 2001;120(1):28-35.
  20. Effect of chlorhexidine varnish application on mutans Streptococci counts in orthodontic patients. Am J Orthod Dentofacial Orthop 2008;133(3):435-39.
  21. Periodontal disease in pregnancy. Correlation between oral hygiene and periodontal condition. Acta Odont Scand 1964;22:21-35.
  22. Evidence that accumulation of mutants in a biofilm reflects natural selection rather than stress-induced adaptive mutation. Appl Environ Microbiol 2007;73:357-61.
  23. International Journal of Paediatric Dentistry 2008;18:446-51.
  24. Multiple exposures to chlorhexidine and xylitol: Adhesion and biofilm formation by Streptococcus mutans. Curr Microbiol 2006;52:418-23.
  25. Retention of chlorhexidine in the human oral cavity after mouthrinses. Arch Oral Biol 1974;19:209-12.
  26. Additive inhibitory effects of combinations of fluoride and chlorhexidine on acid production by Streptococcus mutans and Streptococcus sanguis. Caries Res 1985;19:64-71.
  27. Effect of a combined chlorhexidine and NaF mouthrinse: An in vivo human caries model study. Scand J Dent Res 1994;102:109-12.
  28. Effects of two fluoride varnishes and one fluoride/chlorhexidine varnish on Streptococcus mutans and Streptococcus sobrinus biofilm formation in vitro. Int J Med Sci 2012;9:129-36.
  29. Caries Res 2011;45:162-73.
  30. Caries Res 1991;25(4):292-95.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.