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VOLUME 3 , ISSUE 3 ( July-September, 2012 ) > List of Articles

RESEARCH ARTICLE

The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method

Ehsan Ghasemi, Hamid Badrian, Nafiseh Hosseini, Navid Khalighinejad

Citation Information : Ghasemi E, Badrian H, Hosseini N, Khalighinejad N. The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method. World J Dent 2012; 3 (3):229-233.

DOI: 10.5005/jp-journals-10015-1161

Published Online: 01-09-2012

Copyright Statement:  Copyright © 2012; The Author(s).


Abstract

Background

Dentists, dental equipments and dental laboratories are exposed to different types of pathogenic microorganisms. The aim of this study is to investigate the effect of three different types of disinfectant agents: Sodium hypochlorite 0.525%, Epimax and Deconex, on polyether impressions after 5 and 10 minutes.

Materials and methods

In this in vitro experimental study, 66 circular samples of polyether impression material of 1 cm diameter and 2 mm thickness were contaminated with Staphylococcus aureus (ATCC29213) Pseudomonas aeruginosa (ATCC27853) and Candida albicans fungus (PTCC5027). Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex and Epimax by way of spraying. Afterward, they kept in plastic bags with humid rolled cotton for 5 and 10 minutes. In order to isolate bacteria, the samples were immersed in 2% trypsin for 1 hour and then the solution was diluted with normal salin in portion of 1, 1/2, and 1/4. The trypsin suspensions were transferred to culture plates and the number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. For data analysis Mann-Whitney statistical test was used (α = 0.05).

Results

Epimax and sodium hypochlorite thoroughly eliminated Candida albicans as time elapsed from 5 to 10 minutes; however, the other two microorganisms were not eliminated completely. Deconex was completely efficient for all microorganisms when immersion time was enhanced from 5 to 10 minutes. There was a significant difference between efficacy of Deconex-sodium hypochlorite and sodium hypochlorite-Epimax for Pseudomonas aeruginosa in 5-minute immersion (p-value:0/046) Moreover, Deconex and sodium hypochlorite were significantly different for Staphylococcus aureus (p-value:0/046) and Pseudomonas aeruginosa in 10-minute immersion (p < 0.05).

Conclusion

According to the results of this study, none of the three disinfection materials could eradicate three different kinds of microorganisms in 5 minutes, but in 10 minutes, Deconex could completely eradicate all microorganisms compared to other disinfection agents, which is a good indicator for high efficacy of this agent in disinfecting polyether impressions.

How to cite this article

Ghasemi E, Badrian H, Hosseini N, Khalighinejad N. The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method. World J Dent 2012;3(3):229-233.


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