World Journal of Dentistry

Register      Login

VOLUME 2 , ISSUE 4 ( October-December, 2011 ) > List of Articles

REVIEW ARTICLE

Immunofluorescence in Oral Pathology: Part I—Methodology

BR Premalatha, Roopa S Rao, Vijaya Mysorekar

Citation Information : Premalatha B, Rao RS, Mysorekar V. Immunofluorescence in Oral Pathology: Part I—Methodology. World J Dent 2011; 2 (4):326-331.

DOI: 10.5005/jp-journals-10015-1107

Published Online: 01-12-2011

Copyright Statement:  Copyright © 2011; The Author(s).


Abstract

Immunofluorescence is an immunological method used to demonstrate the presence of antigen and antibodies in tissues or serum. Immunofluorescence techniques have become indispensable in modern pathology laboratories and have greatly contributed to the diagnosis, treatment and understanding of autoimmune bullous diseases, hereditary bullous diseases, collagen-vascular diseases, many inflammatory dermatosis and cutaneous tumors. This review emphasizes the basics of immunofluorescence techniques, methodology, interpretation of the results and the newer advancements in the field.

The application of immunofluorescence techniques in various vesiculobullous disorders will be extensively discussed in the subsequent parts. Part II will deal with the pemphigus group and other dermatological conditions with oral involvement and part III with subepidermal immunobullous diseases.


PDF Share
  1. Techniques of immunofluorescence and their significance. Indian J of Dermatol, Venereol and Leprol 2008;74(4):415-19.
  2. Immunofluorescence of the immunobullous disorders part one: Methodology. Indian J of Dermatol, Venerol and Leprol 1995;61(4):187-95.
  3. Cullings cellular pathology technique. (4th ed), Butterworths 1984.
  4. Bancroft theory and practice of histopathological techniques (5th ed), Churchill Livingstone 2002.
  5. Immunofluorescence in immunobullous diseases. Journal of Pakistan Association of Dermatologists 2003;13:76-88.
  6. Staining Methods (5th Ed), Chapter 10, Immunofluorescence pg 62-65.
  7. Indirect immunofluorescence to demonstrate lichen planus specific antigen (LPSA) in lichen planus. Indian J Dermatol Venerol Leprol 2006;72:350-52.
  8. Oral and maxillofacial pathology. Quintessence Publishing Co, Inc 2003;6.
  9. Advanced diagnostic methods in oral and maxillofacial pathology (Part II): Immunohistochemistry and immunofluorescent methods. Oral Surg Oral Med Oral Pathol Oral Radio Endo 2002;93:56-74.
  10. Lever's histopathology of skin (9th ed), Lippincott Williams and Wilkins Publications, p 64.
  11. Sensitivity of direct immunofluorescence in oral diseases. Study of 125 cases. Med Oral Patol Oral Cir Bucal 1 May 2008;13(5):E287-91.
  12. Effects of anatomic region on immunofluorescence diagnosis of bullous pemphigoid. J Am Acad Dermatol 1985;12:274.
  13. Immunofluorescence in dermatology. Ind J Dermatol 1996;41(2):45-48.
  14. Direct immunofluorescence of oral mucosal biopsies: A comparison of fresh-frozen tissue and formalin-fixed, paraffinembedded tissue. Journal of Oral Pathology and Medicine Sep 1992;21(8):358-63.
  15. Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist. Indian J Dermatol Venereol Leprol 2010;76(2):150-57.
  16. Autoimmune blistering diseases: An update of diagnostic methods and investigations. Clin Exp Dermatol 1994;19:97-112.
  17. Immunofluorescence in dermatology. Int J Dermatol 1993;32:153-61.
  18. Pemphigus in remission: Value of negative direct immunofluorescence in management. J Am Acad Dermatol 1994;30:547-50.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.