Antimicrobial Effect of Selenium-containing Primer in Prevention of White Spot Lesions: An In Vitro Study
P Deepak, AKR S Priya
Keywords :
Cross-sectional microhardness, DenteShield™, Mineral loss, Shear bond strength, Transbond XT
Citation Information :
Deepak P, Priya AS. Antimicrobial Effect of Selenium-containing Primer in Prevention of White Spot Lesions: An In Vitro Study. World J Dent 2024; 15 (5):389-393.
Aim: This study aimed to evaluate the effects of an antimicrobial primer containing selenium on the development of white spot lesions (WSLs) and shear bond strength after artificial caries induction.
Materials and methods: A total of 90 maxillary premolars were divided into two groups, with 40 in each. Group I comprised specimens treated with Transbond XT primer (Etch + Transbond XT primer application + Transbond XT adhesive), while group II consisted of specimens treated with DenteShield™ primer (Etch + DenteShield™ primer + Transbond XT adhesive). The remaining 10 samples underwent only a cross-sectional microhardness test (at 10, 30, 50, 80, and 100 µm) to establish baseline data. After bonding, all samples were stored in artificial saliva for 24 hours. Out of the 40 samples in each group, 30 were subjected to microbial caries induction for 10, 30, and 60 days, respectively, by inoculating dental plaque containing Streptococcus mutans in a medium of brain heart infusion (BHI). These samples were then tested for shear bond strength and cross-sectional microhardness. Both groups were evaluated for their antimicrobial property by agar diffusion test and minimum inhibitory concentration (MIC). Data were recorded and statistically analyzed.
Results: In group I, the microhardness decreased at depths of 10, 30, and 50 µm, with a mineral loss of 50, 36, and 13%, respectively. Comparatively, there was a reduced mineral loss of 18 and 11% for depths of 10 and 30 µm, respectively, in group II. Group II exhibited a shear bond strength of 12.9 ± 2.8 MPa, while group I showed 13.5 ± 1.9 MPa. The shear bond strength between the two groups showed no statistical significance. Group II demonstrated effective antimicrobial properties when evaluated with the agar diffusion method and MIC.
Conclusion: Within the limitations of this study, it was concluded that DenteShield™ inhibited biofilm and reduced WSLs without compromising the shear bond strength.
Clinical significance: The clinical significance of these findings lies in addressing the challenge of preventing WSLs in orthodontics. Therefore, DenteShield™ could serve as a viable alternative to conventional primers in orthodontic bonding procedures, due to its favorable impact on reducing WSLs while maintaining shear bond strength.
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