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VOLUME 15 , ISSUE 5 ( May, 2024 ) > List of Articles


Evaluating the Efficiency of Ultraviolet Light for Removable Denture Disinfection

Prakash Pugazhendhi, Anil Kumar Gujjari, Raghavendraswamy Kudalakuppe Nagaraj, Rashmi P Mahale

Keywords : Denture contamination, Denture disinfection, Denture stomatitis, Removable partial denture, Ultraviolet light disinfection, Ultraviolet rays

Citation Information : Pugazhendhi P, Gujjari AK, Nagaraj RK, Mahale RP. Evaluating the Efficiency of Ultraviolet Light for Removable Denture Disinfection. World J Dent 2024; 15 (5):381-388.

DOI: 10.5005/jp-journals-10015-2426

License: CC BY-NC 4.0

Published Online: 28-06-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Introduction: Denture disinfection is not taken seriously because patients are unaware of the effects of contaminated prostheses on systemic conditions. Among different methods of physical disinfection, ultraviolet (UV) light disinfection has a definite future because of the nondestructive nature of UV light on removable acrylic dentures. Aim: This study aims to “evaluate microbial contamination and compare the efficiency of UV disinfection of acrylic removable dentures with the conventional method of disinfection on contaminated dentures.” Materials and methods: In this study, 45 patients with complete and removable partial dentures were selected and subjected to three different disinfection methods. Group I—UV light-emitting diode (LED) disinfection (wavelength 254 nm) for approximately 300 seconds; group II—brushing with dentifrice; and group III—brushing with tap water for approximately 15–20 strokes. Pre- and postdisinfection microbial swab samples were collected, and the microbiological load in CFU/mL was determined for each sample. Specific microorganisms from the isolated colonies were also identified and analyzed. Results: According to the statistical analysis, there was a positive correlation between pre- and postdisinfection within the groups of disinfection (group I—UV light LED disinfection and group II—brushing with a dentifrice, but not within group III—brushing with tap water). Conclusion: A comparison of UV light disinfection with the other conventional disinfection methods shows a significant reduction in contamination, as measured by postdisinfection microbial colonies, and is comparable with brushing with dentifrices but not with brushing with tap water. Clinical significance: This study provides insights into the efficiency of UV light denture disinfection.

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  1. Ngatia EM, Gathece LW, Macigo FG, et al. Nutritional and oral health status of an elderly population in Nairobi. East African Med J 2008;85(8):378–385. DOI: 10.4314/eamj.v85i8.9655
  2. Coulthwaite L, Verran J. Potential pathogenic aspects of denture plaque. Br J Biomed Sci 2007;64(4):180–189. DOI: 10.1080/09674845.2007.11732784
  3. Manikandan S, Vinesh E, Selvi DT, et al. Prevalence of Candida among denture wearers and nondenture wearers. J Pharm Bioallied Sci 2022;14(Suppl 1):702–705. DOI: 10.4103/jpbs.jpbs_781_21
  4. Orsi IA, Junior AG, Villabona CA, et al. Evaluation of the efficacy of chemical disinfectants for disinfection of heat-polymerised acrylic resin. Gerodontology 2011;28(4):253–257. DOI: 10.1111/j.1741-2358.2010.00400.x
  5. Mima EGDO, Pavarina AC, Ribeiro DG, et al. Effectiveness of photodynamic therapy for the inactivation of Candida spp. on dentures: in vitro study. Photomed Laser Surg 2011;29(12):827–833. DOI: 10.1089/pho.2011.3022
  6. Binns R, Li W, Wu CD, et al. Effect of ultraviolet radiation on Candida albicans biofilm on poly(methylmethacrylate) resin. J Prosthodontics 2020;29(8):686–692. DOI: 10.1111/jopr.13180
  7. Degirmenci BU, Kakai RTH, Guducuoglu H, et al. Evaluation of the effectiveness of current disinfection methods in complete denture patients. Quintessence Int (Berl) 2022;53(1):36–46. DOI: 10.3290/j.qi.b1702307
  8. Paranhos HFO, Silva-Lovato CH, Souza RF, et al. Effects of mechanical and chemical methods on denture biofilm accumulation. J Oral Rehabil 2007;34(8):606–612. DOI: 10.1111/j.1365-2842.2007.01753.x
  9. ISO T. Dental materials guidance on testing of wear: Part 1. Wear by tooth brushing. Technical report 2007.
  10. Sanders ER. Aseptic laboratory techniques: plating methods. J Vis Exp 2012;(63):1–18. DOI: 10.3791/3064
  11. Baba Y, Sato Y, Owada G, et al. Effectiveness of a combination denture-cleaning method versus a mechanical method: comparison of denture cleanliness, patient satisfaction, and oral health-related quality of life. J Prosthodont Res 2018;62(3):353–358. DOI: 10.1016/j.jpor.2018.01.005
  12. Tsutsumi-Arai C, Arai Y, Terada-Ito C, et al. Effectiveness of 405-nm blue LED light for degradation of Candida biofilms formed on PMMA denture base resin. Lasers Med Sci 2019;34(7):1457–1464. DOI: 10.1007/s10103-019-02751-2
  13. Williams DW, Chamary N, Lewis MAO, et al. Microbial contamination of removable prosthodontic appliances from laboratories and impact of clinical storage. Br Dent J 2011;211(4):163–166. DOI: 10.1038/sj.bdj.2011.675
  14. Kaur BN, N AU, Raghunath P, et al. To assess candidal colonization and species diversity in the oral cavity of diabetic and nondiabetic denture wearers and correlation with the presence of candida on finger tips of the patients: an in vivo study. Indian J Health Sci Biomed Res 2014;7(1):45–51. DOI: 10.4103/2349-5006.135041
  15. Pereira CA, Toledo BC, Santos CT, et al. Opportunistic microorganisms in individuals with lesions of denture stomatitis. Diagn Microbiol Infect Dis 2013;76(4):419–424. DOI: 10.1016/j.diagmicrobio.2013.05.001
  16. Arendorf TM, Walker DM. Denture stomatitis: a review. J Oral Rehabil 1987;14(3):217–227. DOI: 10.1111/j.1365-2842.1987.tb00713.x
  17. Shay K, Truhlar MR, Renner RP. Oropharyngeal candidosis in the older patient. J Am Geriatr Soc 1997;45(7):863–870. DOI: 10.1111/j.1532-5415.1997.tb01517.x
  18. Epstein JB, Polsky B. Oropharyngeal candidiasis: a review of its clinical spectrum and current therapies. Clin Therapeut 1998;20(1):40–57. DOI: 10.1016/s0149-2918(98)80033-7
  19. Muzyka BC. Oral fungal infections. Dent Clin North Am 2005;49(1):49–65. DOI: 10.1016/j.cden.2004.07.007
  20. Estrela C, Estrela CRA, Luis Barbin E, et al. Mechanism of action of sodium hypochlorite 113. Braz Dent J 2002;13(2):113–117. DOI: 10.1590/s0103-64402002000200007
  21. Uludamar A, Gökhan Özyeşil A, Ozkan YK. Clinical and microbiological efficacy of three different treatment methods in the management of denture stomatitis. Gerodontology 2011;28(2):104–110. DOI: 10.1111/j.1741-2358.2009.00354.x
  22. Jagger DC, Harrison A. Denture cleansing–the best approach. Br Dent J 1995;178(11):413–417. DOI: 10.1038/sj.bdj.4808788
  23. de Freitas Pontes KM, de Holanda JC, Fonteles CS, et al. Effect of toothbrushes and denture brushes on heat-polymerized acrylic resins. Gen Dent 2016;64(1):49–53. PMID: 26742168.
  24. Campanha NH, Pavarina AC, Brunetti IL, et al. Candida albicans inactivation and cell membrane integrity damage by microwave irradiation. Mycoses 2007;50(2):140–147. DOI: 10.1111/j.1439-0507.2006.01339.x
  25. Rosaspina S, Salvatorelli G, Anzanel D, et al. Effect of microwave radiation on Candida albicans. Microbios 1994;78(314):55–59. PMID: 8022308.
  26. Aslanimehr M, Mojarad N, Ranjbar S, et al. In vitro comparison of the effects of microwave irradiation and chemical and mechanical methods on the disinfection of complete dentures contaminated with Candida albicans. Dent Res J 2018;15(5):340. PMID: 30233654.
  27. Risović D, Maver-Bišćanin M, Mravak-Stipetić M, et al. Quantitative investigation of efficiency of ultraviolet and visible light in eradication of Candida albicans in vitro. Photomed Laser Surg 2014;32(4):232–239. DOI: 10.1089/pho.2013.3691
  28. IESNA. Lighting Handbook: Reference and Application IESNA HB-9-2000. New York: illumination Engineering Society of North America; 2000.
  29. Bettles T, Schujman S, Smart JA, et al. UV light emitting diodes-their applications and benefits. 2007.
  30. Ozcelik B. Fungi/bactericidal and static effects of ultraviolet light in 254 and 354 nm wavelengths. Res J Microbiol 2007;2(1):42–49.
  31. Masschelein WJ, Rice RG. Ultraviolet Light in Water and Wastewater Sanitation. CRC Press; 2016.
  32. Abdulkareem MM. Effectiveness of ultraviolet light on the disinfection of complete dentures. Ann Trop Med Public Health 2020;125–131.
  33. Li J, Hirota K, Yumoto H, et al. Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms. J Appl Microbiol 2010;109(6):2183–2190. DOI: 10.1111/j.1365-2672.2010.04850.x
  34. Sini MK, Abu-Elteen KH, Elkarmi AZ, et al. Influence of various ultraviolet light intensities on pathogenic determinants of Candida albicans. Biotechnology 2007;6(2):210–217. DOI: 10.3923/biotech.2007.210.217
  35. Heath JR, Davenport JC, Jones PA. The abrasion of acrylic resin by cleaning pastes. J Oral Rehabil 1983;10(2):159–175. DOI: 10.1111/j.1365-2842.1983.tb00110.x
  36. Fayaz A, Shakerian M, Ansari G. Comparing the efficiency of Denture brush and Ordinary brush in complete denture cleaning. Novelty Biomed 2013;1(2):62–65. DOI: 10.22037/nbm.v1i2.5248
  37. Nair VV, Karibasappa GN, Dodamani A, et al. Microbial contamination of removable dental prosthesis at different interval of usage: an in vitro study. J Indian Prosthod Soc 2016;16(4):346–351. DOI: 10.4103/0972-4052.176536
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