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VOLUME 15 , ISSUE 5 ( May, 2024 ) > List of Articles


Evaluating the Efficacy of Titanium Dioxide Nanoparticles in Combination with Commonly Used Bleaching Agents: An In Vitro Study

Rajasekhar Vemareddy, Sudhakar Naidu, Bala Raju Korrai, Shanmukha Nagadevara, Someshwar Battu, Jyotsnanjali Thati, Sivaji Kavuri

Keywords : Bleaching, Carbamide peroxide, Hydrogen peroxide, Spectrophotometer, Titanium dioxide nanoparticles

Citation Information : Vemareddy R, Naidu S, Korrai BR, Nagadevara S, Battu S, Thati J, Kavuri S. Evaluating the Efficacy of Titanium Dioxide Nanoparticles in Combination with Commonly Used Bleaching Agents: An In Vitro Study. World J Dent 2024; 15 (5):377-380.

DOI: 10.5005/jp-journals-10015-2422

License: CC BY-NC 4.0

Published Online: 28-06-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: To evaluate the efficacy of titanium dioxide (TiO2) nanoparticles in combination with hydrogen peroxide (H2O2) and carbamide peroxide (CH6N2O3). Materials and methods: A total of 40 freshly extracted maxillary central incisors were discolored by storing them in a freshly prepared coffee solution for 2 weeks and subjected to spectroscopic analysis. Baseline values L1, a1, and b1 of each sample were recorded. The teeth were then randomly divided into four groups (n = 10)—group I was treated with 35% H2O2; group II was treated with TiO2 nanoparticles and 15% H2O2; group III was treated with 15% CH6N2O3; and group IV was treated with TiO2 nanoparticles and 15% CH6N2O3. Immediately after treatment, the samples were analyzed spectroscopically, and the L2, a2, and b2 values of each sample were recorded. The samples were then stored in artificial saliva solution for 7 days and analyzed spectroscopically again, recording L3, a3, and b3 values of each sample. The difference was measured as ΔE*2-1 and ΔE*3-2 for each sample, and the data recorded was statistically analyzed. Results: Mean change in color difference (ΔE*2-1) was higher in group II (2.8200 ± 0.74294) than in group I (2.4300 ± 0.50594) and group IV (2.1360 ± 0.56400) than in group III (2.0910 ± 0.58711). Group II had the highest mean, followed by groups I, IV, and III, respectively. The mean change in color difference (ΔE*3-2) was higher in group II (7.3820 ± 1.31952) than in group I (5.7550 ± 1.18895) and group IV (4.3800 ± 1.56449) than in group III (4.1140 ± 1.00087). Group II had the highest mean, followed by groups I, IV, and III, respectively. The results obtained were statistically significant. Conclusion: The use of TiO2 nanoparticles with lower concentrations of H2O2 and CH6N2O3 has increased the efficiency and longevity of the traditional bleaching agent. Clinical significance: Around 35% of H2O2 is a commonly used bleaching agent, but according to some studies, higher concentrations of H2O2 cause sensitivity in patients. Decreasing the concentration of H2O2 helps decrease the postoperative sensitivity. The addition of nano-TiO2, even with less concentration, gains high efficiency as a bleaching material.

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  1. Douglas RD, Steinhauer TJ, Wee AG. Intraoral determination of the tolerance of dentists for perceptibility and acceptability of shade mismatch. J Prosthet Dent 2007;97(4):200–208. DOI: 10.1016/j.prosdent.2007.02.012
  2. Greenwall L. Bleaching Techniques in Restorative Dentistry: An Illustrated Guide. CRC Press; 2001.
  3. Haywood VB. Nightguard vital bleaching. Quintessence Int 1989;20(3):173–176.
  4. Tran C, Choi E, Watu B, et al. Laboratory model to evaluate efficacy of an experimental titanium oxide nanofibers bleaching agent. Restor Dent Endod 2021;46(4):e47. DOI: 10.5395/rde.2021.46.e47
  5. Kawamoto K, Tsujimoto Y. Effects of the hydroxyl radical and hydrogen peroxide on tooth bleaching. J Endod 2004;30(1):45–50. DOI: 10.1097/00004770-200401000-00010
  6. Andrés CM, Pérez de la Lastra JM, Juan CA, et al. Chemistry of hydrogen peroxide formation and elimination in mammalian cells, and its role in various pathologies. Stress 2022;2(3):256–274. DOI: 10.3390/stresses2030019
  7. Costa CA, Riehl H, Kina JF, et al. Human pulp responses to in-office tooth bleaching. Oral Surg Oral Med Oral Pathol Oral Radiol Endodontol 2010;109(4):e59–e64. DOI: 10.1016/j.tripleo.2009.12.002
  8. Markovic L, Jordan RA, Lakota N, et al. Micromorphology of enamel surface after vital tooth bleaching. J Endod 2007;33(5):607–610. DOI: 10.1016/j.joen.2007.01.011
  9. Tredwin CJ, Naik S, Lewis NJ, et al. Hydrogen peroxide tooth-whitening (bleaching) products: review of adverse effects and safety issues. British Dent J 2006;200(7):371–376. DOI: 10.1038/sj.bdj.4813423
  10. Sulieman MA. An overview of tooth-bleaching techniques: chemistry, safety and efficacy. Periodontology 2000 2008;48(1):148–169. DOI: 10.1111/j.1600-0757.2008.00258.x
  11. Heymann HO. Tooth whitening: facts and fallacies. Br Dent J 2005;198(8):514. DOI: 10.1038/sj.bdj.4812298
  12. Kolsuz Ozcetin H, Surmelioglu D. Three-month evaluation of a low concentration (6% hydrogen peroxide) experimental bleaching gel containing TiO2 and chitosan: an in vitro study. Color Res Appl 2020;45(6):1101–1108. DOI: 10.1002/col.22543
  13. Joiner A. The bleaching of teeth: a review of the literature. J Dent 2006;34(7):412–419. DOI: 10.1016/j.jdent.2006.02.002
  14. Kurzmann C, Verheyen J, Coto M, et al. In vitro evaluation of experimental light activated gels for tooth bleaching. Photochem & Photobiol Sci 2019;18:1009–1019. DOI: 10.1039/C8PP00223A
  15. Lee YK, Yu B, Lim HN, et al. Difference in the color stability of direct and indirect resin composites. J Appl Oral Sci 2011;19(2):154–160. DOI: 10.1590/S1678-77572011000200012
  16. Sakai K, Kato J, Kurata H, et al. The amounts of hydroxyl radicals generated by titanium dioxide and 3.5% hydrogen peroxide under 405-nm diode laser irradiation. Laser Physics 2007;17:1062–1066. DOI: 10.1134/S1054660X07080075
  17. Bortolatto JF, Trevisan TC, Bernardi PS, et al. A novel approach for in-office tooth bleaching with 6% H2O2/TiO_N and LED/laser system—a controlled, triple-blinded, randomized clinical trial. Lasers Med Sci 2016;31(3):437–444. DOI: 10.1007/s10103-016-1866-2
  18. Suemori T, Kato J, Nakazawa T, et al. Effects of light irradiation on bleaching by a 3.5% hydrogen peroxide solution containing titanium dioxide. Laser Phys Lett 2008;5(5):379. DOI: 10.1002/lapl.200710137
  19. Moncada G, Sepúlveda D, Elphick K, et al. Effects of light activation, agent concentration, and tooth thickness on dental sensitivity after bleaching. Operat Dent 2013;38(5):467–476. DOI: 10.2341/12-335-C
  20. Mondelli RF, Francisconi AC, Almeida CM, et al. Comparative clinical study of the effectiveness of different dental bleaching methods-two year follow-up. J Appl Oral Sci 2012;20(4):435–443. DOI: 10.1590/s1678-77572012000400008
  21. Benetti AR, Valera MC, Mancini MN, et al. In vitro penetration of bleaching agents into the pulp chamber. Int Endod J 2004;37(2):120–124. DOI: 10.1111/j.0143-2885.2004.00761.x
  22. Gökay O, Zıraman F, Çalı Asal A, et al. Radicular peroxide penetration from carbamide peroxide gels during intracoronal bleaching. Int Endod J 2008;41(7):556–560. DOI: 10.1111/j.1365-2591.2008.01384.x
  23. Martin J, Fernandez E, Bahamondes V, et al. Dentin hypersensitivity after teeth bleaching with in-office systems. Randomized clinical trial. Am J Dent 2013;26(1):10–14.
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