World Journal of Dentistry

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VOLUME 15 , ISSUE 4 ( April, 2024 ) > List of Articles


Evaluation of In Vitro Cytocompatibility of New Dental Restorative Composite Resin Copolymers Containing 2π + 2π Photodimerized Cinnamyl Methacrylate Crosslinker

Murugesan Sreevarun, Ranganathan Ajay, Nasir Nilofernisha, Jambai S Sivakumar, Suthagar Abhinayaa, Karthigeyan Suma

Keywords : Composite, Crosslinker, Cytotoxicity, Monomeric residuum, Triethylene glycol dimethacrylate

Citation Information : Sreevarun M, Ajay R, Nilofernisha N, Sivakumar JS, Abhinayaa S, Suma K. Evaluation of In Vitro Cytocompatibility of New Dental Restorative Composite Resin Copolymers Containing 2π + 2π Photodimerized Cinnamyl Methacrylate Crosslinker. World J Dent 2024; 15 (4):320-325.

DOI: 10.5005/jp-journals-10015-2398

License: CC BY-NC 4.0

Published Online: 17-05-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: To determine the in vitro cytocompatibility of new dental restorative composite resin (RCR) copolymers containing photodimerized cinnamyl methacrylate (PD-CMA) crosslinker using human pulpal fibroblasts (HPF) by tetrazolium (MTT) assay. Materials and methods: Three research groups were based on the composition of the copolymer. There was a negative control (NC) group only with the cell culture. A total of 27 disk-shaped specimens (n = 9 per group) were prepared. Group C0 (control) consisted of photopolymerized specimens made of base matrix-formers (B), a diluent (D), and without PD-CMA [P(BD)]; experimental groups E10 and E20 consisted of copolymers P(BD-Co-CMA) with 10 wt% PD-CMA substituting D and P(B-Co-CMA) with 20 wt% PD-CMA replacing D, respectively. The specimens were eluted, and an MTT assay was performed. The obtained optical density (OD) values in absorbance unit (AU) were subjected to statistical analysis. Results: The mean OD of C0, E10, and E20 was 0.79, 0.92, and 1.18 AU, respectively. The difference between C0 and E10 was not significant (p = 0.067). The comparisons C0-E20 and E10-E20 were statistically significant (p < 0.05). The order of cytocompatibility was C0 = E10 < E20. Conclusion: The new P(B-Co-CMA) was the most cytocompatible copolymer with HPF in vitro when compared to the P(BD-Co-CMA) and P(BD). Clinical significance: The low-viscosity PD-CMA can eschew the toxic effects of the triethylene glycol dimethacrylate (TEGDMA) by replacing it. Nevertheless, the new copolymer P(B-Co-CMA) with hydrophobic PD-CMA crosslinker would not induce pulpal inflammation and necrosis by preserving the intracellular glutathione and preventing the formation of reactive oxygen species.

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