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VOLUME 15 , ISSUE 3 ( March, 2024 ) > List of Articles

ORIGINAL RESEARCH

In Vitro Evaluation of Microhardness of Hydrophilic Pit and Fissure Sealants Reinforced with Green-synthesized Silver Nanoparticles

Mahalakshmi Kumaraguru, Jayashri Prabakar, Meignana Arumugham Indiran, Ganesh Jeevanandan, Rajesh Kumar Shanmugam

Keywords : Green synthesis, Hydrophilic chemistry, Microhardness, Pit and fissure sealant, Silver nanoparticles

Citation Information : Kumaraguru M, Prabakar J, Indiran MA, Jeevanandan G, Shanmugam RK. In Vitro Evaluation of Microhardness of Hydrophilic Pit and Fissure Sealants Reinforced with Green-synthesized Silver Nanoparticles. World J Dent 2024; 15 (3):257-261.

DOI: 10.5005/jp-journals-10015-2395

License: CC BY-NC 4.0

Published Online: 20-04-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Abstract

Aim: This in vitro study aimed to compare the microhardness of hydrophilic pit and fissure sealants containing green-synthesized silver nanoparticles with hydrophilic sealants and conventional resin sealants on permanent molars. Materials and methods: Silver nanoparticles were green-synthesized using beetroot extract and incorporated into the hydrophilic UltraSeal XT hydro sealant. A total of 33 molars that were extracted were randomly distributed equally to three distinct groups—group I: conventionally used Clinpro sealant, group II: hydrophilic UltraSeal XT Hydro, and group III: UltraSeal XT hydro reinforced with silver nanoparticles, which were then subjected to surface microhardness testing utilizing digital display Vickers microhardness tester. Data were analyzed using Statistical Package for the Social Sciences (SPSS) software (IBM Corp. Released 2015. IBM SPSS Statistics for Windows, Version 23.0. Armonk, New York, United States of America: IBM Corp). Shapiro–Wilks test was used to determine the normality of the data. Kruskal–Wallis test was administered to compare the difference in mean microhardness scores of groups I, II, and III sealants. Results: The difference in microhardness scores [Vickers hardness number (VHN)] of the three groups was statistically significant, with reinforced UltraSeal XT hydro exhibiting the highest value (25.39 ± 1.21), followed by UltraSeal XT hydro (19.65 ± 1.35) and Clinpro sealant (19.62 ± 1.57). Conclusion: UltraSeal XT hydro reinforced with silver nanoparticles (group III) showed the highest microhardness values in comparison with the other groups. Clinical significance: Nanoparticles are gaining attention attributable to their superior physicochemical and biological characteristics, which in turn has facilitated the development of innovative materials with improved functions. Though silver nanoparticles have proven antimicrobial efficacy, their application in pit and fissure sealants is yet to be established beyond doubt. This study has laid the foundation for future research on sealants reinforced with silver nanoparticles.

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  1. Carvalho JC. Caries process on occlusal surfaces: evolving evidence and understanding. Caries Res 2014;48(4):339–346. DOI: 10.1159/000356307
  2. Kühnisch J, Galler M, Seitz M, et al. Irregularities below the enamel-dentin junction may predispose for fissure caries. J Dent Res 2012;91(11):1066–1070. DOI: 10.1177/0022034512458688
  3. Jayachandar D, Gurunathan D, Jeevanandan G. Prevalence of early loss of primary molars among children aged 5–10 years in Chennai: a cross-sectional study. J Indian Soc Pedod Prev Dent 2019;37(2):115–119. DOI: 10.4103/JISPPD.JISPPD_22_19
  4. Jayakumar A, Gurunathan D, Mathew MG. Correlation of protein level with the severity of early childhood caries–an observational study. J Res Med Dent Sci 2020;8(7):534–536.
  5. Gandhi JM, Gurunathan D, Doraikannan S, et al. Oral health status for primary dentition - a pilot study. J Indian Soc Pedod Prev Dent 2021;39(4):369–372. DOI: 10.4103/jisppd.jisppd_155_21
  6. Carvalho JC, Dige I, Machiulskiene V, et al. Occlusal caries: biological approach for its diagnosis and management. Caries Res 2016;50(6):527–542. DOI: 10.1159/000448662
  7. Verma P, Muthuswamy Pandian S. Bionic effects of nano hydroxyapatite dentifrice on demineralised surface of enamel post orthodontic debonding: in-vivo split mouth study. Prog Orthod 2021;22(1):39. DOI: 10.1186/s40510-021-00381-5
  8. Schwendicke F, Jäger AM, Paris S, et al. Treating pit-and-fissure caries: a systematic review and network meta-analysis. J Dent Res 2015;94(4):522–533. DOI: 10.1177/0022034515571184
  9. Swift EJ Jr, Perdigão J, Heymann HO. Bonding to enamel and dentin: a brief history and state of the art. Quintessence Int 1995;26(2):95–110.
  10. Simonsen RJ. The clinical effectiveness of a colored pit and fissure sealant at 36 months. J Am Dent Assoc 1981;102(3):323–327. DOI: 10.14219/jada.archive.1981.0051
  11. Mohapatra S, Prabakar J, Indiran MA, et al. Comparison and evaluation of the retention, cariostatic effect, and discoloration of conventional Clinpro 3M ESPE and hydrophilic UltraSeal XT Hydro among 12–15-year-old schoolchildren for a period of 6 months: a single-blind randomized clinical trial. Int J Clin Pediatr Dent 2020;13(6):688–693. DOI: 10.5005/jp-journals-10005-1859
  12. Prabakar J, Indiran MA, Kumar P, et al. Microleakage assessment of two different pit and fissure sealants: a comparative confocal laser scanning microscopy study. Int J Clin Pediatr Dent 2020;13(Suppl 1):S29–S33. DOI: 10.5005/jp-journals-10005-1862
  13. Khogli AE, Cauwels R, Vercruysse C, et al. Microleakage and penetration of a hydrophilic sealant and a conventional resin-based sealant as a function of preparation techniques: a laboratory study. Int J Paediatr Dent 2013;23(1):13–22. DOI: 10.1111/j.1365-263X.2011.01218.x
  14. Brinker SP. Preventing carious lesions. Clinical steps for applying a newly introduced hydrophilic sealant. Dent Today 2013;32(10):82–83.
  15. Targino AG, Flores MA, dos Santos Junior VE, et al. An innovative approach to treating dental decay in children. A new anti-caries agent. J Mater Sci Mater Med 2014;25(8):2041–2047. DOI: 10.1007/s10856-014-5221-5
  16. Khubchandani M, Thosar NR, Dangore-Khasbage S, et al. Applications of silver nanoparticles in pediatric dentistry: an overview. Cureus 2022;14(7):e26956. DOI: 10.7759/cureus.26956
  17. Favaro JC, Detomini TR, Maia LP, et al. Anticaries agent based on silver nanoparticles and fluoride: characterization and biological and remineralizing effects-an in vitro study. Int J Dent 2022;2022:9483589. DOI: 10.1155/2022/9483589
  18. Mehdizadeh S, Ghasemi N, Ramezani M. The synthesis of silver nanoparticles using beetroot extract and its antibacterial and catalytic activity. Eurasian Chem Commun 2019;1(6):545–558. DOI: 10.33945/SAMI/ECC.2019.6.5
  19. Prabhakar AR, Murthy SA, Sugandhan S. Comparative evaluation of the length of resin tags, viscosity and microleakage of pit and fissure sealants - an in vitro scanning electron microscope study. Contemp Clin Dent 2011;2(4):324–330. DOI: 10.4103/0976-237X.91797
  20. Buonocore MG. A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res 1955;34(6):849–853. DOI: 10.1177/00220345550340060801
  21. Borges BC, Bezerra GV, Mesquita Jde A, et al. Effect of irradiation times on the polymerization depth of contemporary fissure sealants with different opacities. Braz Oral Res 2011;25(2):135–142. DOI: 10.1590/s1806-83242011000200007
  22. Mitra SB, Wu D, Holmes BN. An application of nanotechnology in advanced dental materials. J Am Dent Assoc 2003;134(10):1382–1390. DOI: 10.14219/jada.archive.2003.0054
  23. Alkheraif AA, AlMufareh NA, AlQhtani FA, et al. Minocycline incorporated cobalt oxide nanoparticles in dental sealants: An in vitro study. Microsc Res Tech 2022;85(7):2558–2566. DOI: 10.1002/jemt.24109
  24. Kamangar HSS, Zareian H, Bahador A, et al. Evaluation of antibacterial effects of fissure sealants containing Chitosan nanoparticles. Int J Dent 2021;2021:8975948. DOI: 10.1155/2021/8975948
  25. Yasir M, Singh J, Tripathi MK, et al. Green synthesis of silver nanoparticles using leaf extract of common arrowhead houseplant and its anticandidal activity. Pharmacogn Mag 2018;13(Suppl 4):S840–S844. DOI: 10.4103/pm.pm_226_17
  26. Ahmed S, Ahmad M, Swami BL, et al. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 2016;7(1):17–28. DOI: 10.1016/j.jare.2015.02.007
  27. Mandhalkar R, Paul P, Reche A. Application of nanomaterials in restorative dentistry. Cureus 2023;15(1):e33779. DOI: 10.7759/cureus.33779
  28. Mota EG, Oshima HMS, Burnett LH Jr, et al. Evaluation of diametral tensile strength and Knoop microhardness of five nanofilled composites in dentin and enamel shades. Stomatologija 2006;8(3):67–69.
  29. Salas-López EK, Pierdant-Pérez M, Hernández-Sierra JF, et al. Effect of silver nanoparticle-added pit and fissure sealant in the prevention of dental caries in children. J Clin Pediatr Dent 2017;41(1):48–52. DOI: 10.17796/1053-4628-41.1.48
  30. Osorio E, Osorio R, Davidenko N, et al. Polymerization kinetics and mechanical characterization of new formulations of light-cured dental sealants. J Biomed Mater Res B Appl Biomater 2007;80(1):18–24. DOI: 10.1002/jbm.b.30563
  31. Güçlü ZA, Dönmez N, Hurt AP, et al. Characterisation and microleakage of a new hydrophilic fissure sealant - UltraSeal XT® hydro™. J Appl Oral Sci 2016;24(4):344–351. DOI: 10.1590/1678-775720160010
  32. Sulimany AM, BinSaleh SS, AlYahya E, et al. Effect of aging on the microhardness of different resin based fluoride-releasing fissure sealants: an in vitro study. J Contemp Dent Pract 2022;22(10):1144–1149. DOI: 10.5005/jp-journals-10024-3153
  33. Beun S, Bailly C, Devaux J, et al. Physical, mechanical and rheological characterization of resin-based pit and fissure sealants compared to flowable resin composites. Dent Mater 2012;28(4):349–359. DOI: 10.1016/j.dental.2011.11.001
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