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VOLUME 15 , ISSUE 2 ( February, 2024 ) > List of Articles


Chemopreventive Role of Sulindac Sulfone on 4-nitroquinoline 1-oxide-induced Head and Neck Squamous Cell Carcinogenesis in Rats

Nivethitha Karuppiah, S Gheena, Pratibha Ramani, Abilasha Ramasubramanian

Keywords : β-catenin, E-cadherin, Head and neck squamous cell carcinoma, Histopathology, Immunohistochemistry, 4-nitroquinoline 1-oxide, Sulindac sulfone

Citation Information : Karuppiah N, Gheena S, Ramani P, Ramasubramanian A. Chemopreventive Role of Sulindac Sulfone on 4-nitroquinoline 1-oxide-induced Head and Neck Squamous Cell Carcinogenesis in Rats. World J Dent 2024; 15 (2):141-149.

DOI: 10.5005/jp-journals-10015-2370

License: CC BY-NC 4.0

Published Online: 02-04-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: The objective of this study was to investigate the efficacy of sulindac sulfone against head and neck squamous cell carcinoma (HNSCC) induced with 4-nitroquinoline 1-oxide (4NQO) in a murine model. Materials and methods: A total of 18 male Wistar albino rats were divided into three groups (n = 6) as follows—group I (control), which received normal drinking water; group II (test), which received 4-NQO through topical application and drinking water; and group III (treatment) which received 4-NQO followed by with sulindac sulfone (100 mg/kg). At the end of the experimental period in the 21st week, the rats were sacrificed, the tissues were collected, formalin-fixed, and sections were made. Histopathological analysis and immunohistochemical (IHC) expression of e-cadherin and β-catenin were analyzed. Results: Histopathological analysis showed improvement in the histopathological parameters in the treatment group; however, it was not statistically significant. IHC analysis showed increased β-catenin (p = 0.296) and increased E-cadherin expression (p = 0.002) in treatment compared to the test group. Conclusion: The study data indicate that sulindac sulfone displayed showed effective chemoprotective activity in 4-NQO induced HNSCC mediated by improvement in histopathology parameters and upregulation in the expression of E-cadherin and β-catenin. Clinical significance: Sulindac sulfone elicits potent chemoprotective activity against 4-NQO provoked HNSCC mediated by activation of pro-inflammatory condition and apoptosis.

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