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VOLUME 15 , ISSUE 2 ( February, 2024 ) > List of Articles


Biosynthesis of Zinc Oxide Nanoparticles and Its Cytotoxic Evaluation Using Human Gingival Fibroblast Cell Lines

Mary S Missier, Mahesh Ramakrishnan, Saravana Dinesh, Deepa Gurunathan

Keywords : Cytotoxic testing, Fibroblast, Human gingival fibroblast cells, Lemon juice, Zinc oxide nanoparticles

Citation Information : Missier MS, Ramakrishnan M, Dinesh S, Gurunathan D. Biosynthesis of Zinc Oxide Nanoparticles and Its Cytotoxic Evaluation Using Human Gingival Fibroblast Cell Lines. World J Dent 2024; 15 (2):102-106.

DOI: 10.5005/jp-journals-10015-2366

License: CC BY-NC 4.0

Published Online: 02-04-2024

Copyright Statement:  Copyright © 2024; The Author(s).


Aim: To synthesize zinc oxide (ZnO) nanoparticles (NPs) and to evaluate their cytotoxicity using human gingival fibroblast cell lines. Materials and methods: Zinc oxide (ZnO) NP was synthesized from lemon extract, and an 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was used to measure the cytotoxic effects of ZnO NPs on a human gingival fibroblast cell line. The percentage of surviving cells was determined 24 hours after being exposed to ZnO NPs at 10, 20, 30, 50, 100, and 200 ng/mL. Results: Zinc oxide (ZnO) NPs demonstrated roughly 84% cytotoxicity on human gingival fibroblast cells, even at the highest dose tested (200 ng/mL), compared to the control group with 100% cell viability. The MTT assay showed that after 24 hours of treatment with 100 nm, ZnONP did not significantly alter the cell viability in comparison to the control group, showing it to be less cytotoxic. Conclusion: Zinc oxide (ZnO) NPs are perfectly suited for targeted drug administration due to their regulated size, shape, and surface features. They are also safe for usage due to their biocompatibility and dose-dependent cytotoxic effect. Clinical significance: Zinc oxide (ZnO) NPs synthesized from the above method can be used in various biomedical applications. The lemon extract is an alternative method to the conventional synthesis of silver NPs.

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  1. Lee NY, Ko WC, Hsueh PR. Nanoparticles in the treatment of infections caused by multidrug-resistant organisms. Front Pharmacol 2019;10:1153. DOI: 10.3389/fphar.2019.01153
  2. Blecher K, Nasir A, Friedman A. The growing role of nanotechnology in combating infectious disease. Virulence 2011;2(5):395–401. DOI: 10.4161/viru.2.5.17035
  3. Hajipour MJ, Saei AA, Walker ED, et al. Nanotechnology for targeted detection and removal of bacteria: opportunities and challenges. Adv Sci (Weinh) 2021;8(21):e2100556. DOI: 10.1002/advs.202100556
  4. Makabenta JMV, Nabawy A, Li CH, et al. Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections. Nat Rev Microbiol 2021;19(1):23–36. DOI: 10.1038/s41579-020-0420-1
  5. Malik S, Waheed Y. Emerging applications of nanotechnology in dentistry. Dentistry Journal 2023;11(11):266. DOI: 10.3390/dj11110266.
  6. Zakrzewski W, Dobrzynski M, Dobrzynski W, et al. Nanomaterials application in orthodontics. Nanomaterials (Basel) 2021;11(2):337. PMID: 33525572.
  7. Jasso-Ruiz I, Velazquez-Enriquez U, Scougall-Vilchis RJ, et al. Silver nanoparticles in orthodontics, a new alternative in bacterial inhibition: in vitro study. Prog Orthod 2020;21(1):24. DOI: 10.1186/s40510-020-00324-6
  8. De Stefani A, Bruno G, Preo G, et al. Application of nanotechnology in orthodontic materials: a state-of-the-art review. Dent J (Basel) 2020;8(4): DOI: 10.3390/dj8040126
  9. Mishra PK, Mishra H, Ekielski A, et al. Zinc oxide nanoparticles: a promising nanomaterial for biomedical applications. Drug Discov Today 2017;22(12):1825–1834. DOI: 10.1016/j.drudis.2017.08.006
  10. Singh TA, Das J, Sil PC. Zinc oxide nanoparticles: a comprehensive review on its synthesis, anticancer and drug delivery applications as well as health risks. Adv Colloid Interface Sci 2020;286:102317. DOI: 10.1016/j.cis.2020.102317
  11. Thounaojam TC, Meetei TT, Devi YB, et al. Zinc oxide nanoparticles (ZnO-NPs): a promising nanoparticle in renovating plant science. Acta Physiologiae Plantarum 2021;43:1–21. DOI: 10.1007/s11738-021-03307-0
  12. Hossain A, Abdallah Y, Ali MA, et al. Lemon-fruit-based green synthesis of zinc oxide nanoparticles and titanium dioxide nanoparticles against soft rot bacterial pathogen dickeya dadantii. Biomolecules 2019;9(12). DOI: 10.3390/biom9120863
  13. Mahiuddin M, Ochiai B. Comprehensive study on lemon juice-based green synthesis and catalytic activity of bismuth nanoparticles. ACS Omega 2022;7(40):35626–35634. DOI: 10.1021/acsomega.2c03416
  14. Vankar PS, Shukla D. Biosynthesis of silver nanoparticles using lemon leaves extract and its application for antimicrobial finish on fabric. Appl Nanosci 2012;2:163–168. DOI: 10.1007/s13204-011-0051-y
  15. Luu TL, Cao XT, Nguyen VT, et al. Simple controlling ecofriendly synthesis of silver nanoparticles at room temperature using lemon juice extract and commercial rice vinegar. J Nanotechnol 2020;2020:1–9. DOI: 10.1155/2020/3539701
  16. Wen S, Hui Y, Chuang W. Biosynthesis and antioxidation of nano-selenium using lemon juice as a reducing agent. Green Process Synth 2021;10(1):178–188. DOI: 10.1515/gps-2021-0018
  17. Poonguzhali RV, Kumar ER, Sumithra MG, et al. Natural citric acid (lemon juice) assisted synthesis of ZnO nanostructures: evaluation of phase composition, morphology, optical and thermal properties. Ceramic Int 2021;47(16):23110–23115. DOI: 10.1016/j.ceramint.2021.05.024
  18. Selim YA, Azb MA, Ragab I, et al. Green synthesis of zinc oxide nanoparticles using aqueous extract of deverra tortuosa and their cytotoxic activities. Sci Rep 2020;10(1):3445. DOI: 10.1038/s41598-020-60541-1
  19. Iqbal J, Abbasi BA, Yaseen T, et al. Green synthesis of zinc oxide nanoparticles using Elaeagnus angustifolia L. leaf extracts and their multiple in vitro biological applications. Sci Rep 2021;11(1):20988. DOI: 10.1038/s41598-021-99839-z
  20. Naseer M, Aslam U, Khalid B, et al. Green route to synthesize zinc oxide nanoparticles using leaf extracts of Cassia fistula and Melia azadarach and their antibacterial potential. Sci Rep 2020;10(1):9055. DOI: 10.1038/s41598-020-65949-3
  21. Wang Q, Mei S, Manivel P, et al. Zinc oxide nanoparticles synthesized using coffee leaf extract assisted with ultrasound as nanocarriers for mangiferin. Curr Res Food Sci 2022;5:868–877. DOI: 10.1016/j.crfs.2022.05.002
  22. Aldalbahi A, Alterary S, Ali Abdullrahman Almoghim R, et al. Greener synthesis of zinc oxide nanoparticles: characterization and multifaceted applications. Molecules 2020;25(18): DOI: 10.3390/molecules25184198
  23. Sanaeimehr Z, Javadi I, Namvar F. Antiangiogenic and antiapoptotic effects of green-synthesized zinc oxide nanoparticles using Sargassum muticum algae extraction. Cancer Nanotechnol 2018;9(1):3. DOI: 10.1186/s12645-018-0037-5
  24. Król A, Pomastowski P, Rafińska K, et al. Zinc oxide nanoparticles: synthesis, antiseptic activity and toxicity mechanism. Adv Colloid Interface Sci 2017;249:37–52. DOI: 10.1016/j.cis.2017.07.033
  25. Vanlalveni C, Lallianrawna S, Biswas A, et al. Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Adv 2021;11(5):2804–2837. DOI: 10.1039/d0ra09941d
  26. Braun K, Stürzel CM, Biskupek J, et al. Comparison of different cytotoxicity assays for in vitro evaluation of mesoporous silica nanoparticles. Toxicol In Vitro 2018;52:214–221. DOI: 10.1016/j.tiv.2018.06.019
  27. Selvaraj S, Chokkattu JJ, Shanmugam R, et al. Anti-inflammatory potential of a mouthwash formulated using clove and ginger mediated by zinc oxide nanoparticles: an in vitro study. World J Dent 2023;14(5):394–401. DOI: 10.5005/jp-journals-10015-2232
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