Aim: To compare and evaluate the efficacy of two in-office bleaching systems with and without laser activation. Materials and methods: A total of 75 extracted anterior teeth were collected, cleaned, and stored in distilled water at 37°C for 60 days. The specimens were artificially stained using a tea solution. The solution was renewed each day, and the specimens were stained to a uniform shade of C4 as judged visually and by a spectrophotometer. The teeth were divided into five groups of 15 each—group I, control group; group II, bleaching using Pola Office bleaching system; group III, bleaching using Opalescence Boost; group IV, Pola Office activated with diode laser; and group V, Opalescence Boost activated with diode laser. The color evaluation was done both prior to and after the procedure and the resultant data was subjected to statistical analysis. Results: The multiple comparisons of the mean difference of ΣE between groups, showed that group II (p = 0.002) and III (p = 0.003) showed a significantly lesser mean ΣE value as compared to group IV (p = 0.007), and V (p = 0.03). Similar results were observed for L values. For C and H, group III demonstrated better results followed by groups V, II, and IV. Conclusion: Within the limits of this study, groups with laser activation demonstrated greater improvement in color for the evaluated parameters compared to groups without laser activation with the Opalescence Boost and laser group showing the best results. Clinical significance: Lasers can serve as valuable adjuncts to traditional bleaching techniques as they significantly enhance the results.