Aim: To assess the impact of dental fluorosis on the oral health-related quality of life (OHRQoL) in 10–14-year-old children. Materials and methods: The present study included a total of 545 children aged 10–14 years. All the children were screened to note dean fluorosis indices, decayed, missing, filled teeth (DMFT), and oral hygiene index-simplified (OHI-S) followed by which, children were asked to answer the Child Perception Questionnaire (CPQ) in their local language. Screening at schools ended with an oral healthcare talk. The data collected was compiled, tabulated, and subjected to statistical analysis. Results: Among the 545 subjects in the study 58.3% were males and 41.7% were females. The mean Dean fluorosis index score was 2.48 ± 0.77. The means of domains oral symptoms, functional limitations, emotional well-being, and social well-being were 2.41 ± 2.56, 2.14 ± 2.70, 3.73 ± 4.58, and 4.38 ± 5.55, respectively. Multiple regression analysis of the data showed that dental fluorosis had a significant impact on OHRQoL in children. A comparison of OHRQoL scores between various Dean's fluorosis index scores shows that OHRQoL was mostly affected in children with severe fluorosis followed by moderate fluorosis. Conclusion: Dental fluorosis has a negative impact on the OHRQoL of children. An increase in the degree of fluorosis has a more negative effect on the OHRQoL. Dental fluorosis mainly affects the children's social and emotional well-being. Clinical significance: Fluorosis affects the OHRQoL of children, suitable health education and preventive measures can be provided. It is essential to take precautions to lower the danger of fluoride exposure. Parents can be educated about the causes of dental fluorosis, preventive measures, and management.