Aim: To evaluate and compare microbial leakage occurring in three different popularly used implant-abutment connections (IAC). Materials and methods: Nine implant analogs and abutments have a Conelog connection (BioHorizons, United States of America), nine implant analog and abutments have a Conexa connection (B&B, Italy), and nine implant analog and abutments have an Internal octagon connection (Osstem, Korea) were immersed in bacterial peptone broth containing Staphylococci bacteria for 14 days. After this time, the implant analog abutment assemblies were disassembled, and swabs were taken from the internal surface of the connection, which were then cultured and evaluated for growth. Results: Microbial leakage was seen in 15 out of 27 samples. Conclusion: Higher quantities of bacterial ingress were seen in the Conexa and Internal octagon connections. However, the differences were not found to be statistically significant. Clinical significance: The implant abutment connections is one of the most crucial components of the implant-prosthesis system, especially when it comes to the longevity of the treatment. When this IAC is subjected to occlusal loading, micromovements of the abutment occur, which perpetuate a microgap between the abutment and implant. This microgap can cause leakage of microorganisms, which would then act as a bacterial reservoir. This gap is located at the level of the alveolar crest. This could lead to unfavorable biological consequences such as inflammation and infection, leading to peri-implantitis and increased crestal bone loss, which could ultimately lead to implant failure.