Minimal Inhibitory Concentration and Minimal Bactericidal Concentration of Various Intracanal Medicaments against Enterococcus faecalis: Microbial Analysis
Citation Information :
Babu AK. Minimal Inhibitory Concentration and Minimal Bactericidal Concentration of Various Intracanal Medicaments against Enterococcus faecalis: Microbial Analysis. World J Dent 2023; 14 (8):696-699.
Aim: The aim of the study was to compare the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of three intracanal medicaments against Enterococcus faecalis (E. faecalis).
Materials and methods: Three intracanal medicament groups were used in the current study. Each group had five samples. Group I—calcium hydroxide paste, group II—triple antibiotic paste (TAP) (metronidazole, ciprofloxacin, minocycline), group III—double antibiotic paste (DAP) (metronidazole, ciprofloxacin). The nutrient agar was then stored at room temperature for 2 days before use to verify that it had remained sterile. Brain heart infusion broth was also prepared and stored in 5 mL vials for 2 days. The serial dilution was done with concentrations ranging from for each intracanal medicament in the other test tubes. The lowest dilution inhibiting the growth was taken as MIC. A loopful of the broth dilutions was taken and streaked on nutrient agar plates. The growth of bacteria was checked after incubation for 24 hours at 37°C. The lowest dilution which showed no growth of bacteria was taken as the minimum bactericidal concentration.
Results: Growth of E. faecalis was found to be inhibited even with the lowest dilution of the TAP itself which was 1.8 ± 0.7 µgm per mL while it was a little higher for calcium hydroxide paste with a dilution of 4.39 ± 1.6 µgm per mL and of 5.01 ± 1.69 µgm per mL for DAP (p < 0.05). While the bactericidal effect was seen at 2.19 ± 0.8 µgm per mL for triple antibiotic paste, 4.39 ± 1.69 µgm per mL for CaOH, and 5.63 ± 1.38 µgm per mL for DAP (p < 0.05).
Conclusion: It had been found that MIC and MBC were least for TAP compared to other intracanal medicaments. The addition of minocycline in a TAP increased the antimicrobial property.
Clinical significance: For successful root canal treatment choosing effective intracanal medicament is important to debride the microorganism completely even at lower concentration. The present study provides a minimum concentration of some intracanal medicament used in root canal treatment.
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