Aim: The study aims to assess the wettability of three different commercially available types of mouthwash—Betadine, chlorhexidine (CHX), and herbal mouthwash (HiOra) using contact angle goniometer. Materials and methods: A total of 30 anterior teeth was sectioned longitudinally to produce 10 mm long, thin enamel plates with intact labial surfaces and divided into three groups corresponding to the three different types of mouthwash (Betadine, CHX, and HiOra mouthwash) with 10 enamel plates in each group. Using the Ossila goniometer, the contact angle for each droplet was measured from the base of the enamel plate section and the line angle at which the droplet makes contact with the tooth surface. The wettability of the samples was evaluated as it is inversely proportional to the contact angle with the facial surface of the microtomed teeth. One-way analysis of variance (ANOVA) test was used to compare the contact angle measurements among the three different types of mouthwash and multiple intergroup comparisons were done using Tukey's post hoc test. Results: HiOra mouthwash had the lowest average contact angle of 15.7980 than Betadine (17.9600) and CHX (23.5340) and the results were statistically significant (p-value of 0.0070). Conclusion: The results showed that the wettability of HiOra was more when compared to Betadine and CHX and thus further studies are to be evaluated to determine its beneficial activities. Clinical significance: Low contact-angle values demonstrate the tendency of the mouthwash to spread and adhere to the surface. Numerous biological processes depend heavily on surface wettability of mouthwashes. Evaluating the wettability of commonly prescribed mouthwashes is crucial in determining the efficacy of these mouthwashes for their biological actions.