Citation Information :
Basavaraju S, Raghavan VH, Priyadarshini V, Yadav AK. Effect of Low-level Laser Therapy on the Viability and Proliferation of Gingival Mesenchymal Stem Cells: An Ex Vivo Pilot Study. World J Dent 2023; 14 (3):254-258.
Aim: The present ex vivo study was designed to assess the in vitro effects of low-level laser therapy (LLLT) on the viability and proliferation of gingival mesenchymal stem cells (GMSCs).
Materials and methods: Gingival explants were obtained from the gingival collar of teeth advised for minor gingival surgical procedures. Following processing, the GMSCs were irradiated with a 660 nm diode laser according to the following groups. Group I—1 J/cm2, 25 mW, and 40 seconds; group II—2 J/cm2, 50 mW, and 10 seconds; and group III—no irradiation (control group). 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was done to assess the rate of cell proliferation by measuring the absorbance values using a spectrometer. The mean values of absorbance in each of the three groups were considered for statistical analysis separately at 12 and 24 hours, respectively.
Results: The absorbance values of tetrazolium reduction were directly proportional to the rate of cell proliferation. Both groups I and II showed statistically significant differences in the absorbance rates from 12 to 24 hours after irradiation. Both at 12 and 24 hours after irradiation, group I exhibited a greater absorbance value compared to group II and this difference was statistically significant (p < 0.05).
Conclusion: Low-level laser therapy (LLLT) using 660 nm diode laser with different energies showed a positive effect on in vitro proliferation of GMSCs. The rate of proliferation was comparatively more significant at 12 hours and lower energy.
Clinical significance: This study provides a basis for the probable application of LLLT as a tool in tissue engineering using GMSCs.
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