World Journal of Dentistry

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VOLUME 14 , ISSUE 10 ( October, 2023 ) > List of Articles


Efficacy of Chemically and Biologically Synthesized Zinc Oxide Nanoparticles Incorporated in Soft Denture Liner against Candida albicans: A Comparative In Vitro Study

Laura APM Albuquerque, Sunila B Sangappa, Asha Srinivasan, Ann Archer, Raghavendra Rao

Keywords : Azadirachta indica (Neem), Candida albicans, Denture stomatitis, Soft liner, Zinc oxide nanoparticles

Citation Information : Albuquerque LA, Sangappa SB, Srinivasan A, Archer A, Rao R. Efficacy of Chemically and Biologically Synthesized Zinc Oxide Nanoparticles Incorporated in Soft Denture Liner against Candida albicans: A Comparative In Vitro Study. World J Dent 2023; 14 (10):851-859.

DOI: 10.5005/jp-journals-10015-2311

License: CC BY-NC 4.0

Published Online: 07-11-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Aim: The present study aimed to comparatively evaluate the efficacy of varying concentrations of chemically synthesized zinc oxide nanoparticles (CSZnO-NPs) and biologically synthesized ZnO-NPs (BSZnO-NPs) incorporated separately in soft denture liners against Candida albicans (C. albicans). Materials and methods: The sample size for this experimental comparative in vitro study was 120. ZnO-NPs were biologically synthesized from Azadirachta indica (A. indica) (neem) leaves and characterized by ultraviolet visible (UV-vis), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray powder diffraction (XRD). CSZnO-NPs were procured from Sigma Aldrich. CSZnO-NPs and BSZnO-NPs were then incorporated separately into soft liner in concentrations of 0 (control), 3, 5, 7, 9, and 11 wt% for the purpose of comparison. The disk diffusion method was used to evaluate antifungal activity and the zones of inhibition (ZOI) were measured. Data was compiled and analyzed using the Statistical Package for the Social Sciences (SPSS) software version 20. Inferential statistics used were analysis of variance (ANOVA) and independent t-test. A p-value of <0.05 was considered significant. Results: Characterized BSZnO-NPs subjected to SEM showed particles of <100 nm in size with spherical morphology, UV-vis spectroscopy showed the absorption peak at a wavelength of 220 nm, XRD showed a good crystalline structure formation, and FTIR showed the presence of functional groups. For antifungal evaluation, the mean ZOI were measured, and an ANOVA test was performed, which showed a significant difference (p < 0.0001) between the concentrations of CSZnO-NPs and BSZnO-NPs incorporated in soft liners, respectively. Least significant difference (LSD) post hoc analysis showed a significant difference (p < 0.001) between the various concentrations, with the most significant difference seen at 7 wt% of CSZnO-NPs and 9 wt% of BSZnO-NPs. On performing an independent t-test, it was inferred that 3, 5, 7, and 11 wt% incorporation of CSZnO-NPs showed significantly higher ZOI against C. albicans as compared to the respective concentrations of BSZnO-NPs. At 9 wt%, there was no significant difference between the two groups. Conclusion: In conclusion, the optimal antifungal concentration of CSZnO-NPs and BSZnO-NPs is 7 and 9 wt%, respectively, when incorporated into the soft liner. Overall, CSZnO-NPs proved to be more efficacious than BSZnO-NPs against C. albicans. Clinical significance: Incorporation of optimal concentrations of CSZnO-NPs or BSZnO-NPs into soft denture liner is efficacious in combating the incidence of denture stomatitis (DS). Valuing the advantages of green synthesis, BSZnO-NPs can also be recognized for their antifungal efficacy.

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  1. Cunha-Cruz J, Hujoel PP, NadanovsḰy P. Secular trends in socio-economic disparities in edentulism: USA, 1972-2001. J Dent Res 2007;86(2):131–136. DOI: 10.1177/154405910708600205
  2. Daniluk T, Tokajuk G, Stokowska W, et al. Occurrence rate of oral Candida albicans in denture wearer patients. Adv Med Sci 2006;51(Suppl 1):77–80. PMID: 17458064.
  3. Bhat V, Sharma SM, Shetty V, et al. Prevalence of Candida associated denture stomatitis (cads) and speciation of candida among complete denture wearers of South West coastal region of Karnataka. J Heal Allied Sci NU 2013;03(3):59–63. DOI: 10.1055/s-0040-1703679
  4. Pereira-Cenci T, Del Bel Cury AA, Crielaard W, et al. Development of Candida-associated denture stomatitis: new insights. J Appl Oral Sci 2008;16(2):86–94. DOI: 10.1590/s1678-77572008000200002
  5. Abualsaud R, Aleraky DM, Akhtar S, et al. Antifungal activity of denture base resin containing nanozirconia: in vitro assessment of Candida albicans biofilm. Scientific World Journal 2021;2021:5556413. DOI: 10.1155/2021/5556413
  6. Contaldo M, Romano A, Mascitti M, et al. Association between denture stomatitis, Candida species and diabetic status. J Biol Regul Homeost Agents 2019;33(3):35–42. PMID: 31538448.
  7. Saeedi P, Petersohn I, Salpea P, et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pract 2019;157:107843. DOI: 10.1016/j.diabres.2019.107843
  8. Rao CR, Kamath VG, Shetty A, et al. A study on the prevalence of type 2 diabetes in coastal Karnataka. Int J Diabetes Dev Ctries 2010;30(2):80–85. DOI: 10.4103/0973-3930.62597
  9. Dorocka-Bobkowska B, Budtz-Jörgensen E, Wtoch S. Non-insulin-dependent diabetes mellitus as a risk factor for denture stomatitis. J Oral Pathol Med 1996;25(8):411–415. DOI: 10.1111/j.1600-0714.1996.tb00288.x
  10. Kreve S, Dos Reis AC. Denture liners: a systematic review relative to adhesion and mechanical properties. Sci World J 2019;2019:6913080. DOI: 10.1155/2019/6913080
  11. Kusama T, Aida J, Yamamoto T, Kondo K, et al. Infrequent denture cleaning increased the risk of pneumonia among community-dwelling older adults: a population-based cross-sectional study. Sci Rep 2019;9(1):13734. DOI: 10.1038/s41598-019-50129-9
  12. Vural C, Ozdemir G, Kurtulmus H, et al. Comparative effects of two different artificial body fluids on candida albicans adhesion to soft lining materials. Dent Mater J 2010;29(2):206–212. DOI: 10.4012/dmj.2009-081
  13. Akpan A, Morgan R. Oral candidiasis. Postgrad Med J 2002;78(922):455–459. DOI: 10.1136/pmj.78.922.455
  14. Mousavi SA, Ghotaslou R, Akbarzadeh A, et al. Evaluation of antibacterial and antifungal properties of a tissue conditioner used in complete dentures after incorporation of ZnO–Ag nanoparticles. J Dent Res Dent Clin Dent Prospects 2019;13(1):11. DOI: 10.15171/joddd.2019.002
  15. Ansarifard E, Zareshahrabadi Z, Sarafraz N, et al. Evaluation of antimicrobial and antibiofilm activities of copper oxide nanoparticles within soft denture liners against oral pathogens. Bioinorg Chem Appl 2021;2021:9939275. DOI: 10.1155/2021/9939275
  16. Ahmed AQ, Ali M. The influence of titanium dioxide nanoparticles incorporation into soft denture lining material on candida albicans adherence and some mechanical and physical properties. J Pure Appl Microbiol 2018;12(2):783–791. DOI: 10.22207/JPAM.12.2.39
  17. Mousavi SA, Ghotaslou R, Khorramdel A, et al. Antibacterial and antifungal impacts of combined silver, zinc oxide, and chitosan nanoparticles within tissue conditioners of complete dentures in vitro. Ir J Med Sci 2020;189(4):1343–1350. DOI: 10.1007/s11845-020-02243-1
  18. Hosseini SS, Ghaemi E, Koohsar F. Influence of ZnO nanoparticles on Candida albicans isolates biofilm formed on the urinary catheter. Iran J Microbiol 2018;10(6):424–432. PMID: 30873271.
  19. Dobrucka R, Długaszewska J. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. Saudi J Biol Sci 2016;23(4):517–523. DOI: 10.1016/j.sjbs.2015.05.016
  20. Mahmoud DA, Hassanein NM, Youssef KA, et al. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. Braz J Microbiol 2011;42(3):1007–1016. DOI: 10.1590/S1517-838220110003000021
  21. Bajunaid SO. How effective are antimicrobial agents on preventing the adhesion of Candida albicans to denture base acrylic resin materials? A systematic review. Polymers (Basel) 2022;14(5):908. DOI: 10.3390/polym14050908
  22. Karthika D, Vimala T. Green synthesis of zinc oxide nanoparticles using Azadirachta indica leaves extract and its characterization and application in the degradation of methylene blue. Int J Sci Res 2018;7(8):1175–1177. DOI: 10.21275/ART2019823
  23. Pillai AM, Sivasankarapillai VS, Rahdar A, et al. Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. J Mol Struct 2020;1211:128107. DOI: 10.1016/j.molstruc.2020.128107
  24. Singh A, Neelam, Kaushik M. Physicochemical investigations of zinc oxide nanoparticles synthesized from Azadirachta indica (Neem)leaf extract and their interaction with Calf-Thymus DNA. Results Phy 2019;13:102168. DOI: 10.1016/j.rinp.2019.102168
  25. Elumalai K, Velmurugan S. Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L.). Appl Surf Sci 2015;345:329–336. DOI: 10.1016/j.apsusc.2015.03.176
  26. Hashem MI. Advances in soft denture liners: an update. J Contemp Dent Pract 2015;16(4):314–318. DOI: 10.5005/jp-journals-10024-1682
  27. Moradpoor H, Safaei M, Mozaffari HR, et al. An overview of recent progress in dental applications of zinc oxide nanoparticles. RSC Adv 2021;11(34):21189–21206. DOI: 10.1039/d0ra10789a
  28. Sirelkhatim A, Mahmud S, Seeni A, et al. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett 2015;7(3):219–242. DOI: 10.1007/s40820-015-0040-x
  29. Parveen K, Banse V, Ledwani L. Green synthesis of nanoparticles: their advantages and disadvantages. AIP Conf Pro 2016;1724. DOI: 10.1063/1.4945168
  30. Gour A, Jain NK. Advances in green synthesis of nanoparticles. Artif Cells Nanomed Biotechnol 2019;47(1):844–851. DOI: 10.1080/21691401.2019
  31. Homsiang W, Kamonkhantikul K, Arksornnukit M, et al. Effect of zinc oxide nanoparticles incorporated into tissue conditioner on antifungal, physical, and mechanical properties. Dent Mater J 2021;40(2):481–486. DOI: 10.4012/dmj.2020-095
  32. Subapriya R, Nagini S. Medicinal properties of neem leaves: a review. Curr Med Chem - Anticancer Agents 2005;5(2):149–156. DOI: 10.2174/1568011053174828
  33. Govindachari TR, Suresh G, Gopalakrishnan G, et al. Identification of antifungal compounds from the seed oil of Azadirachta indica. Phytoparasitica 1998;26(2):109–116. DOI: 10.1007/BF02980677
  34. Noorjahan CM, Shahina SKJ, Deepika T, et al. Green synthesis and characterization of zinc oxide nanoparticles from neem (Azadirachta indicia). Int J Sci Eng Technol Res 2015;4(30):5757–5753.
  35. Rajendran SP, Sengodan K. Synthesis and characterization of zinc oxide and iron oxide nanoparticles using sesbania grandiflora leaf extract as reducing agent. J Nanosci 2017;2017(17):1–7. DOI: 10.1155/2017/8348507
  36. El Badri Nagla El Badri MS, Sulieman KM. Optical absorption measurement of synthesized ZnO using ultra violet-visible spectrophotometer. Int J Sci Res 2015;4(11):62–65.
  37. Bhuyan T, Mishra K, Khanuja M, et al. Biosynthesis of zinc oxide nanoparticles from Azadirachta indica for antibacterial and photocatalytic applications. Mater Sci Semicond Process 2015;32:55–61. DOI: 10.1016/j.mssp.2014.12.053
  38. Kreve S, Oliveira VC, Bachmann L, et al. Influence of AgVO3 incorporation on antimicrobial properties, hardness, roughness and adhesion of a soft denture liner. Sci Rep 2019;9(1):11889. DOI: 10.1038/s41598-019-48228-8
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