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VOLUME 13 , ISSUE S2 ( Supplementary Issue 2, 2022 ) > List of Articles

ORIGINAL RESEARCH

Evaluation of Antifungal Activity of Moringa oleifera Seeds on Oral Candida Isolated from Type 2 Diabetic and Nondiabetic Complete Denture Wearers

Tanvi Tekwani, Sunila Bukanakere Sangappa, SubbaRao V Madhunapantula, Tejashree Anantha Balraj Urs, Krishna Karthik

Keywords : Antifungal agents, Antifungal resistance, Candida, Ethanolic extract, Moringa oleifera seed coat, Oral candidiasis, Type 2 diabetes

Citation Information :

DOI: 10.5005/jp-journals-10015-2157

License: CC BY-NC 4.0

Published Online: 31-12-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Aim: To evaluate the antifungal potential of Moringa oleifera seed extracts on Candida isolated from oral cavities of diabetic (case) and nondiabetic (control) complete denture wearers. Materials and methods: Serial exhaustive extraction of M. oleifera seed coat and seed endosperm was done using hexane, dichloromethane, and ethanol. The salivary samples collected from the oral cavities of edentulous complete denture wearers were evaluated for susceptibility with M. oleifera seed coat and endosperm extracts on 60 salivary samples diabetic [case group, 30 individuals hemoglobin A1C (HbA1c) >6.4%] and nondiabetic (control group, 30 individuals HbA1c <6.4%). The potent extract's mean percent inhibition was compared using an independent t-test. The mean zone of inhibition was compared using an independent t-test between diabetic (case group) and nondiabetic (control group) groups using Statistical Package for Social Sciences (SPSS) 24 software, and a p-value of <0.05 was statistically significant. The potent M. oleifera extracts were tested for their in vitro anti-inflammatory potential. Results: M. oleifera seed coat ethanolic extract of 1000 mg/mL concentration showed a zone of inhibition of 20 mm against positive control nystatin (NYS) with a zone of 19 mm is the most potent antifungal agent. The susceptibility of Candida isolated from the saliva of nondiabetics (control) was higher when compared to that isolated from diabetics (case group). The anti-inflammatory potential of the potent extract revealed a dose-dependent % inhibition. Conclusion: The current study provides a potential herbal alternative in the form of M. oleifera seed coat extract against oral Candida. The study also explains the low toxicity of the M. oleifera ethanolic extract on human erythrocytes, making it a safer alternative to NYS. Clinical significance: Oral candidiasis due to the overgrowth of Candida in denture wearers is common. Herbal alternative to conventional antifungal agents against oral Candida that are effective and safe is successfully discussed in this study.

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