World Journal of Dentistry

Register      Login

VOLUME 13 , ISSUE 3 ( May-June, 2022 ) > List of Articles

ORIGINAL RESEARCH

Biomineralized Limpet Radular Teeth as Functionalized Bio-fillers in Experimental Dental Composite Resin

Dhanasekaran Sihivahanan, Venugopal V Nandini, Tripuravaram VK Reddy

Keywords : Bio-filler, Composite resin, Limpet radula, Strength

Citation Information : Sihivahanan D, Nandini VV, Reddy TV. Biomineralized Limpet Radular Teeth as Functionalized Bio-fillers in Experimental Dental Composite Resin. World J Dent 2022; 13 (3):201-206.

DOI: 10.5005/jp-journals-10015-2006

License: CC BY-NC 4.0

Published Online: 11-04-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Abstract

Aim and objective: To develop and analyze the mechanical properties of a silanized limpet radula as a bio-filler in an experimental composite resin. The objective is to compare the compressive, tensile, and flexural strength of the experimental composite resin with bio-filler and the Universal Hybrid Composite resin. Materials and methods: Littoraria scabra species have been selected and used in the study. The tongue-like radula was extracted from the buccal mass under an optical microscope. The radula was then subjected to SEM and EDX analysis. The experimental dental composite resin with 2.5% of radula as silanized bio-filler was prepared based on previous studies and SEM analysis was done to verify the filler morphology. The experimental composite resin was compared with the Universal Hybrid Composite resin. Based on ISO 4049, the samples were prepared and 15 samples from each group were then randomly subdivided into three subgroups to analyze the compressive, tensile, and flexural strength. Results: Statistical analysis were conducted using the Shapiro-Wilk and an independent t-test. Compared to universal hybrid composite resin, the experimental composite resin with the bio-filler had relatively high compressive, tensile, and flexural strength. Conclusion: Within the limitations of the analysis, it can be concluded that the addition of 2.5% of silanized limpet radula as bio-filler in experimental composite resin has improved mechanical properties than the Universal Hybrid Composite. Clinical significance: The extreme mechanical properties shown by limpet radula can be used as a bio-filler to improve the mechanical properties of composite resin.


PDF Share
  1. Barber AH, Lu D, Pugno NM. Extreme strength observed in limpet teeth. J R Soc Interface 2015;12(105):1–5. DOI: 10.1098/rsif.2014.1326
  2. Sone ED, Weiner S, Addadi L. Morphology of goethite crystals in developing Limpet teeth: assessing biological control over mineral formation.Cryst Growth Des 2005;5(6):2131–2138. DOI: 10.1021/cg050171l
  3. Hua T E, Li C W. Silica biomineralization in the radula of a limpet Notoacmea schrenckii (Gastropoda: Acmaeidae). Zool Stud 2007;46(4):379–388.
  4. Thomaidis S, Kakaboura A, Mueller WD, et al. Mechanical properties of contemporary composite resins and their interrelations. Dent Mater 2013;29(8):e132–e141. DOI: 10.1016/j.dental.2013.04.025
  5. Raorane D V, Chaughule R S, Pednekar S R, et al. Experimental synthesis of size-controlled TiO 2 nanofillers and their possible use as composites in restorative dentistry. Saudi Dent J 2019;31(2):194–203. DOI: 10.13039/501100001405
  6. Janssen H H, Triebskorn R. Comparative morphology of the radulae in Pomatia elegans and Littorina littorea. Zool Anz 1987;219(2):73–82.
  7. Mikovari A, Williams J, Oakes F, et al. Radula development in the giant key-hole Limpet Megathura crenulata.J Shellfish Res 2015;34(3):893–902. DOI: 10.2983/035.034.0319
  8. Sihivahanan D, Nandini V V. Comparative evaluation of mechanical properties of titanium dioxide nanoparticle incorporated in composite resin as a core restorative material. J Contemp Dent Pract 2021;22(6):686–690.
  9. Rodríguez H A, Kriven W M, Casanova H. Development of mechanical properties in dental resin composite: effect of filler size and filler aggregation state. Mater Sci Eng C 2019;101(52):274–282. DOI: 10.1016/j.msec.2019.03.090
  10. Hosseinalipour M, Javadpour J, Rezaie H, et al. Investigation of mechanical properties of experimental Bis-GMA/TEGDMA dental composite resins containing various mass fractions of silica nanoparticles. J Prosthodont 2010;19(2):112–117. DOI: 10.1111/j.1532-849X.2009.00530.x
  11. Heintze S D,Ilie N, Hickel R, et al. Laboratory mechanical parameters of composite resins and their relation to fractures and wear in clinical trials—a systematic review. Dent Mater 2017;33(3):e101–e114. DOI: 10.1016/j.dental.2016.11.013
  12. Kim KH, Ong JL, Okuno O. The effect of filler loading and morphology on the mechanical properties of contemporary composites. J Prosthet Dent 2002;87(6):642–649. DOI: 10.1067/mpr.2002.125179
  13. Ukmar-Godec T, Kapun G, Zaslansky P, et al. The giant keyhole limpet radular teeth: a naturally-grown harvest machine. J Struct Biol 2015;192(3):392–402. DOI: 10.1016/j.jsb.2015.09.021
  14. Padilla DK. Form and function of radular teeth of herbivorous molluscs: focus on the future. Am Malacol Bull 2003;18(1):1–5.
  15. Shaw J A, Macey D J, Brooker L R. Radula synthesis by three species of iron mineralizing molluscs: Production rate and elemental demand. J Mar Biol Assoc U K 2008;88(3):597–601. DOI: 10.1017/s0025315408000969
  16. Sihivahanan D, Nandini V, Rajesh E, et al. Goethite nanofibers as bio-fillers in dental composite resin. Indian J Forensic Med Toxicol 2020;14(4):1488–1491. DOI: 10.37506/ijfmt.v14i4.11750
  17. Guralnick R, De Maintenon M J. Formation and homology of radular teeth; a case study using columbellid gastropods (Neogastropoda: Columbellidae). J Molluscan Stud 1997;63(1):65–77. DOI: 10.1093/mollus/63.1.65
  18. Ramdas R, Kumar R, Balan P, et al. Comparative evaluation of compressive and flexural strength of newer nanocomposite materials with conventional hybrid composites- an invitro study. IOSR J Dent Med Sci2017;16(12):65–69. DOI: 10.9790/0853-1612046569
  19. Nica I, Iovan G, Stoleriu S, et al. Comparative study regarding the compressive strength of different composite resins used for direct restorations. Mater Plast 2018;55(3):447–453. DOI: 10.37358/MP.18.3.5049
  20. Mirica I C, Furtos G, Bâldea B, et al. Influence of filler loading on the mechanical properties of flowable resin composites. Materials (Basel) 2020;13(6):1–11. DOI: 10.3390/ma13061477
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.