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VOLUME 13 , ISSUE 2 ( March-April, 2022 ) > List of Articles


Evaluation of Cytocompatibility of Thermopolymerized Denture Base Copolymer Containing a Novel Ring-opening Oxaspiro Comonomer

Ramajayam Sasikala, Jayaraman Raghunathan, Vellingiri LalithaManohari, Kandasamy Baburajan

Keywords : Cell viability, Copolymer, Cytocompatibility, Cytotoxicity, Ring-opening

Citation Information : Sasikala R, Raghunathan J, LalithaManohari V, Baburajan K. Evaluation of Cytocompatibility of Thermopolymerized Denture Base Copolymer Containing a Novel Ring-opening Oxaspiro Comonomer. World J Dent 2022; 13 (2):127-132.

DOI: 10.5005/jp-journals-10015-1901

License: CC BY-NC 4.0

Published Online: 31-01-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Aim and objective: To evaluate the cytocompatibility of a novel denture base copolymer processed with 3,9-dimethylene-1,5,7,11- tetraoxaspiro[5,5]undecane (DMTOSU) comonomer using human keratinocytes (HKCs) and gingival fibroblasts (HGFs) by tetrazolium assay. Materials and methods: The specimens were grouped based on the composition of the resultant polymer and curing regimen employed. Nine disk-shaped specimens per group were polymerized by one of the following curing regimens. GCW: plain poly (methyl methacrylate) [P(MMA)] polymerized by short curing cycle in water-bath without DMTOSU; GTW: P(MMA-Co-DMTOSU)W copolymer polymerized with 20 wt% DMTOSU at 70°C for 2 h followed by short curing cycle in water bath; GTA: P(MMA-Co-DMTOSU)A copolymer polymerized with 20 wt% DMTOSU at 60°C for 45 min followed by 130°C for 20 min in an autoclave. Human keratinocytes and HGFs were employed to evaluate cell viability (CV%) by elution method through tetrazolium assay. Results: A statistically significant difference was obtained (p < 0.05) among the groups with both the cell types. The ascending order of cytocompatibility is GCW < GTW < GTA with the CV% > 70%. Conclusion: The novel P(MMA-Co-DMTOSU) denture copolymer is found to be more cytocompatible with HKC and HGF than the P(MMA). Clinical significance: The novel P(MMA-Co-DMTOSU) denture base copolymer cytocompatible to HKCs and HGFs might bypass polymerization shrinkage and food accumulation at denture–tissue interface. Therefore, this copolymer is also anticipated to prevent oral malodor and stomatitis due to good tissue adaptability and dimensional accuracy.

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