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VOLUME 13 , ISSUE 2 ( March-April, 2022 ) > List of Articles


Development and Evaluation of Silver Nanosystem-based Novel Antimicrobial Dental Stone

Jalis Aaisha Khan, Kishore Ginjupalli, Nagaraj Perampally Upadhya, Nishanth Balachandran Bhat, Tarun Awatramaney, Saleemulla Khan, Mamta Ballal

Keywords : Antimicrobial activity, Copper sulfate, Dental stone, Disinfectants, Inhibitory zone, Potassium permanganate, Silver nitrate

Citation Information : Khan JA, Ginjupalli K, Upadhya NP, Bhat NB, Awatramaney T, Khan S, Ballal M. Development and Evaluation of Silver Nanosystem-based Novel Antimicrobial Dental Stone. World J Dent 2022; 13 (2):104-109.

DOI: 10.5005/jp-journals-10015-1914

License: CC BY-NC 4.0

Published Online: 31-01-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Aim: To find a suitable disinfectant additive with effective antimicrobial activity and physical properties to dental gypsum product. Methods and materials: Design and settings of the study involved three compounds; Silver nitrate (0.3%, 0.5%, 1%); Copper Sulfate (0.5% and 1%), and Potassium Permanganate (0.5% and 1%). Antimicrobial properties, setting time, compressive strength (1 hour and 24 hours) and reproduction of surface details were evaluated. Standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans were used in the study. Dental stone was mixed with test compounds and plain water (control) were sampled in punch well on agar plates which were incubated at 37°C for 24 hours. Mean of Minimum Inhibitory Zone (MIZ) in mm, was measured at 24 hours. The setting time was measured by standard indentation, Compressive strength using Universal testing machine and reproduction of surface details as per ADA guidelines. Statistical analysis of the data was done using one-way ANOVA with Tukey's post hoc test using Graph pad Prism Version 5. p-value of < 0.05 was considered as statistically significant. Results: Silver nitrate showed excellent antimicrobial activity against all the microorganisms. The setting time with 0.5% Silver nitrate was between 30 and 34 minutes, the compressive strength was found to be greater than the control and the surface details were acceptable. Thus, 0.5% silver nitrate was reported as an alternative disinfectant for gypsum products. Conclusion: An amount of 0.5% silver nitrate could be safely suggested as suitable disinfectant additive for gypsum product. Clinical significance: The antimicrobial property imparted to the stone reduces the risk of cross-infection among dental personnel, patients and lab personnel from bacterial, viral, and fungal microorganisms that remain even viable after cleansing and disinfecting the impressions.

  1. Centers for Disease Control and Prevention. Guidelines for Infection Control in Dental Health-Care Settings - 2003. MMWR 2003; 52 (No. RR-17).
  2. Runnells RR. An overview of infection control in dental practice. J Prosthet Dent 1988; 59(5):625–629. DOI: 10.1016/0022-3913(88)90083-2
  3. British Dental Association, Advice sheet, infection control in dentistry. Centers for Disease control and prevention. Guidelines for Infection Control in Dental Health Care Setting- 2003. 2003/52(RR17);1–97.
  4. Anderson, JN. Applied dental materials. Third ed. Blackwell scientific publications. Gypsum Products for Dental Casts 1972;179.
  5. Marya CM, Shukla P, Dahiya V, et al. Current status of disinfection of dental impressions in Indian dental colleges: a cause of concern. J Infect Dev Ctries 2011;5(11):776–780. DOI: 10.3855/jidc.1652
  6. Leung RL, Schonfeld SE. Gypsum casts as a potential source of microbial cross-contamination. J Prosthet Dent 1983;49(2):210–211. DOI: 10.1016/0022-3913(83)90503-6
  7. Kawahara K, Tsuruda K, Morishita M, et al. Antibacterial effect of silver-zeolite on oral bacteria under anaerobic conditions. Dent Mater 2000;16(6):452–455. DOI: 10.1016/s0109-5641(00)00050-6
  8. Pandian SR, Deepak V, Kalishwaralal K, et al. Mechanism of bactericidal activity of Silver Nitrate-a concentration dependent bi-functional molecule. Braz J Microbiol 2010;41(3):805–809. DOI: 10.1590/s1517-83822010000300033
  9. Yang G Xiao, Guo-wei HZ, Chen L. Dimensional accuracy of dental gypsum casts after immersion in stable chlorine dioxide disinfectant Zhejiang Da Xue Xue Bao Yi Xue Ban 2010; 39 (3):318–321.
  10. Goh KT, Chan YW, Womg LYM, et al. The prevalence of hepatitis B virus Markers in dental personnel in Singapore. Trans R Soc Trop Med Hyg 1988; 82(6)908–910. DOI: 10.1016/0035-9203(88)90038-7
  11. Ammon A, Reichart PA, Pauli G, et al. Hepatitis band C among Berlin Dental Personnel: incidence, risk factors and effectiveness of barrier prevention measures. 2000;125(2)407–413. DOI: 10.1017/s0950268899004537
  12. Tebrock OC, Engelmeier RL, Mayfield TG, et al. Managing dental impressions and casts of patients with communicable diseases. Gen Dent 1989;37(6):490–495.
  13. Thiruvenkadam G, Asokan S, John B, et al. Effect of 95% ethanol as a final irrigant before root canal obturation in primary teeth: an in vitro study. Int J Clin Pediatr Dent 2016;9(1):21–24. DOI: 10.5005/jp-journals-10005-1327
  14. Abbaszadegan A, Khayat A, Motamedifar M. Comparison of antimicrobial efficacy of IKI and NaOCl irrigants in infected root canals: an in vivo study. Iran Endod J 2010;5 (3):101–106
  15. Türkün M, Türkün Ç, Kalender A. Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins. Quintessence Int 2004;35(6):469–476.
  16. Schecter A, Pavuk M, Päpke O, et al. Potassium dichromate and ethyl alcohol as blood preservatives for analysis of chlorinated organics. Chemosphere 2003; 60:154–157. DOI: 10.1016/j.chemosphere.2004.04.062
  17. Sharma V, Nainan MT, Shivanna V. The effect of cavity disinfectants on the sealing ability of dentin bonding system: an in vitro study. J Conserv Dent 2009;12(3):109–113. DOI: 10.4103/0972-0707.57634
  18. Thosar N, Basak S, Bahadure RN, et al. Antimicrobial efficacy of five essential oils against oral pathogens: an in vitro study. Eur J Dent 2013;7(S 01):S071–S077. DOI: 10.4103/1305-7456.119078
  19. Cruz-Filho AM, Sousa-Neto MD, Savioli RN, et al. Effect of chelating solutions on the microhardness of root canal lumen dentin. J Endod 2011;37(3):358–362. DOI: 10.1016/j.joen.2010.12.001
  20. Pthalates an overview science direct topics https;// and planetary science/phthalates (W.J.G.M. Peijnenburg, in Encyclopedia of Ecology, 2008)
  21. Therapeutic composition formulated as a dental rinse that stimulates Prostaglandin synthesis in the mouth to prevent plaque buildup on the teeth and Periodontal disease patent by Inventor John E. McHugh.
  22. Delgado-Enciso I, Madrigal-Perez VM, Lara-Esqueda A, et al. Topical 5% potassium permanganate solution accelerates the healing process in chronic diabetic foot ulcers. Biomed Rep 2018;8(2):156–159. DOI: 10.3892/br.2018.1038
  23. Bonev B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother 2008;61(6):1295–1301. DOI: 10.1093/jac/dkn090
  24. Amin WM, Al-Ali MH, Al Tarawneh SK, et al. The effects of disinfectants on dimensional accuracy and surface quality of impression materials and gypsum casts. J Clin Med Res 2009;1(2):81–89. DOI: 10.4021/jocmr2009.04.1235
  25. Lu H, Nguyen B, Powers JM. Mechanical properties of 3 hydrophilic addition silicone and polyether elastomeric impression materials. J Prosthet Dent 2004; 92(2):151–154. DOI: 10.1016/j.prosdent.2004.05.016
  26. Lucas MG, Arioli-Filho JN, Nogueira SS, et al. Effect of incorporation of disinfectant solutions on setting time, linear dimensional stability, and detail reproduction in dental stone casts. J Prosthodont 2009;18(6):521–526. DOI: 10.1111/j.1532-849X.2009.00466.x
  27. Zakaria MR, Johnston WM, Reisbick MH, et al. The effect of the liquid dispensing agent and a microcrystalline additive on the physical properties of Type IV gypsum. J Pros Dent 1988; 60(5):630–637.
  28. Russell AD, Day MJ Antibacterial activity of chlorhexidine, J Hosp Infect 1993; 25(4);229–238.
  29. Labeeb RM, Jaffer NT. Effectiveness of some disinfection solutions on compressive strength and Hardness properties of two types of dental stones JODR 2019;6;(2):47–58.
  30. Jaffer NT. Effectiveness ofsome disinfectant solutions on the compressive strength and hardness properties of two types of dental stone. JODR 2019;6(2).
  31. Powers, JM, Sakaguchi, RL. (2006) Craig's Restorative Dental Materials 12th Edition, Mosby, Missouri, 2006.386–393.
  32. Kenneth J. Anusavice, Phillps’ Science of Dental Materials 11th Edition Philidelphia, Saunders an Imprint of Elsevier.2003.269.
  33. ISO 6873. Dental gypsum products. International Organization for Standardization. 3rd ed. Geneva: Switzerland; 2013.
  34. Taqa AA, Mohammed NZ, Bash TY. The effect of adding some chemical materials on the water powder ratio of dental stone. IJAHMR 2018; 2(7): 13–17.
  35. Abdelaziz KM, Combe EC, Hodges JS. The effect of disinfectants on the properties of dental gypsum: 1. mechanical properties. J Prosthodont 2002;11(3):161–167. DOI: 10.1053/jopr. 2002.126860
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