World Journal of Dentistry

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VOLUME 13 , ISSUE 1 ( January-February, 2022 ) > List of Articles


Evaluation of Interleukin 6 Levels in Gingival Crevicular Fluid and Periodontal Ligament on Application of Orthodontic Forces

Akhil Shetty, Mahima Jain, K Sneha, Veena Shetty, Shama Rao, Ashutosh Shetty

Keywords : ELISA, Gingival crevicular fluid, Interleukin 6, Orthodontic force, Periodontal ligament

Citation Information : Shetty A, Jain M, Sneha K, Shetty V, Rao S, Shetty A. Evaluation of Interleukin 6 Levels in Gingival Crevicular Fluid and Periodontal Ligament on Application of Orthodontic Forces. World J Dent 2022; 13 (1):16-20.

DOI: 10.5005/jp-journals-10015-1891

License: CC BY-NC 4.0

Published Online: 29-12-2021

Copyright Statement:  Copyright © 2022; The Author(s).


Aim and objective: The purpose of this study was to assess and compare the interleukin 6 levels in gingival crevicular fluid and periodontal ligament before and after application of the orthodontic force. Materials and methods: It is a split mouth study where the gingival crevicular fluid (GCF) and periodontal ligament (PDL) samples of 20 individuals were collected from control and experimental site. GCF samples were collected using 1–5 μL calibrated volumetric microcapillary pipette. PDL samples were collected from the extracted tooth, and PDL tissue was scraped using Gracey curette and was immediately transferred to Eppendorf microcentrifuge tubes (Sigma–Aldrich; St. Louis, MO, USA). The collected samples were stored at −80°C for further analysis using ELISA kit (Human IL-6 ELISA MAXTM Delux-5 plates) and the optical density was recorded at 450 nm. Result: In the study it was observed that there was an increase in IL-6 concentration in the PDL in the experimental group (3rd day of force application). The concentration of IL-6 was recorded after 3rd day of force application between the experimental groups of PDL and GCF and an increase in the levels of IL-6 was noted. The increase in the level of IL-6 in these groups was statistically insignificant. Conclusion: The study depicted similar concentration level of IL-6 in GCF and PDL on the 3rd day of application of orthodontic force. Therefore, GCF can be used as a biomarker in the assessment of IL-6 for up to 3 days of force application instead of PDL. Clinical significance: GCF can be assessed to know the concentration of cytokines (IL-6) upto 3 days of force application in the PDL, as collection of GCF is a non-invasive technique.

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