World Journal of Dentistry

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VOLUME 12 , ISSUE 4 ( July-August, 2021 ) > List of Articles


Assessment of DNA Damage of Periodontal Tissues in Premalignant Condition: By Comet Assay

Anubha M Raj, Surekha Rathod, Abhay P Kolte, Pranjali V Bawankar

Keywords : DNA, Oral submucous fibrosis, Periodontitis, Premalignant lesions

Citation Information : Raj AM, Rathod S, Kolte AP, Bawankar PV. Assessment of DNA Damage of Periodontal Tissues in Premalignant Condition: By Comet Assay. World J Dent 2021; 12 (4):316-321.

DOI: 10.5005/jp-journals-10015-1850

License: CC BY-NC 4.0

Published Online: 15-07-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Aim and objective: The present study aimed to determine the effect of OSMF on periodontium and DNA damage in affected and healthy subjects. Materials and methods: Sixty patients (20 patients with OSMF and periodontitis, 20 patients having only OSMF, and 20 controls) were examined clinically with regard to probing pocket depth (PPD), clinical attachment level (CAL), and gingival recession (GR). Radiovisiographs were obtained for the assessment of alveolar bone level. Comet assay was used for the assessment of DNA damage by evaluating tail length, percentage of DNA in the tail, and olive tail moment. Results: Mean PPD, CAL, and GR of patients having both OSMF and periodontitis were 4.21, 5.75, and 1.6 mm, respectively, which was significantly higher than PPD, CAL, and GR of the other two groups. The mean alveolar bone loss was 5.94, 0.57, and 0.51 mm for groups I, II, and III, respectively. Mean tail DNA percent was 49.77, 44.55, and 11.01 and the olive tail moment was 14.16, 12.12, and 2.50 for groups I, II, and III, respectively. The mean tail parameters for the OSMF population were significantly higher than healthy group. Conclusion: OSMF was found to have an adverse effect on periodontal tissue and the severity of DNA damage was more in patients having OSMF with periodontitis. Clinical significance: Comet assay is a noninvasive, rapid, and inexpensive indicator for the assessment of DNA damage in periodontal tissue of OSMF patients with periodontitis.

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