Aim and objective: The present study aimed to determine the effect of OSMF on periodontium and DNA damage in affected and healthy subjects.
Materials and methods: Sixty patients (20 patients with OSMF and periodontitis, 20 patients having only OSMF, and 20 controls) were examined clinically with regard to probing pocket depth (PPD), clinical attachment level (CAL), and gingival recession (GR). Radiovisiographs were obtained for the assessment of alveolar bone level. Comet assay was used for the assessment of DNA damage by evaluating tail length, percentage of DNA in the tail, and olive tail moment.
Results: Mean PPD, CAL, and GR of patients having both OSMF and periodontitis were 4.21, 5.75, and 1.6 mm, respectively, which was significantly higher than PPD, CAL, and GR of the other two groups. The mean alveolar bone loss was 5.94, 0.57, and 0.51 mm for groups I, II, and III, respectively. Mean tail DNA percent was 49.77, 44.55, and 11.01 and the olive tail moment was 14.16, 12.12, and 2.50 for groups I, II, and III, respectively. The mean tail parameters for the OSMF population were significantly higher than healthy group.
Conclusion: OSMF was found to have an adverse effect on periodontal tissue and the severity of DNA damage was more in patients having OSMF with periodontitis.
Clinical significance: Comet assay is a noninvasive, rapid, and inexpensive indicator for the assessment of DNA damage in periodontal tissue of OSMF patients with periodontitis.
Ekanayaka RP, Tilakaratne WM. Oral submucous fibrosis: review on mechanisms of malignant transformation. Oral Surg Oral Med Oral Pathol Oral Radiol 2016;122(2):192–199. DOI: 10.1016/j.oooo.2015.12.018.
Dodani K, Anumala N, Avula H, et al. Periodontal findings in patients with oral submucous fibrosis and comet assay of affected gingival epithelial cells. J Periodontol 2012;83(8):1038–1047. DOI: 10.1902/jop.2011.110352.
Sirsat SM, Khanolkar VR. Submucous fibrosis of the palate in diet preconditioned wister rats Induction by local painting of capsaicin - an optical and electron microscopic study. Arch Patho 1960;l70:171–179.
Murti PR, Bhonsle RB, Gupta PC, et al. Etiology of oral submucous fibrosis with special reference to the role of areca nut chewing. J Oral Pathol Med 1995;24(4):145–152. DOI: 10.1111/j.1600-0714.1995.tb01156.x.
Chiu CJ, Chiang CP, Chang ML, et al. Association between genetic polymorphism of tumor necrosis factor-alpha and risk of oral submucous fibrosis, a precancerous condition of oral cancer. J Dent Res 2001;80(12):2055–2059. DOI: 10.1177/00220345010800120601.
Oliver AJ, Radden BG. Oral submucous fibrosis. Case report and review of the literature. Aust Dent J. 1992;37(1):31–34. DOI: 10.1111/j.1834-7819.1992.tb00830.x.
Canniff JP, Harvey W, Harris M. Oral submucous fibrosis: its pathogenesis and management. Br Dent J 1986;160(12):429–434. DOI: 10.1038/sj.bdj.4805876.
Hazarey VK, Erlewad DM, Mundhe KA, et al. Oral submucous fibrosis: study of 1000 cases from central India. J Oral Pathol Med 2007;36(1):12–17. DOI: 10.1111/j.1600-0714.2006.00485.x.
Ariyawardana A, Athukorala AD, Arulanandam A. Effect of betel chewing, tobacco smoking and alcohol consumption on oral submucous fibrosis: a case-control study in Sri Lanka. Oral Pathol Med. 2006;35(4):197–201. DOI: 10.1111/j.1600-0714.2006.00400.x.
Ho WH, Lee YY, Chang LY, et al. Effects of areca nut extract on the apoptosis pathways in human neutrophils. J Periodont Res. 2010;45(3):412–420. DOI: 10.1111/j.1600-0765.2009.01253.x.
de Miranda CM, van Wyk CW, van der Biji P, et al. The effect of areca nut on salivary and selected oral microorganisms. Int Dent J 1996;46(4):350–356.
Hung SL, Chen YL, Wan HC, et al. Effects of areca nut extracts on the functions of human neutrophils in vitro. J Periodontal Res 2000;35(4):186–193. DOI: 10.1034/j.1600-0765.2000.035004186.x.
Waddington RJ, Moseley R, Embery G. Reactive oxygen species: a potential role in pathogenesis of periodontal diseases. Oral Dis 2000;6(3):138–151. DOI: 10.1111/j.1601-0825.2000.tb00325.x.
Govindaraj P, Khan NA, Gopalakrishna P, et al. Mitochondrial dysfunctionand genetic heterogeneity in chronic periodontitis. Mitochondrion. 2011;11(3):504–512. DOI: 10.1016/j.mito.2011. 01.009.
Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4(1):1–6. DOI: 10.1902/annals.1918.104.22.168.
Khanna JN, Andrade NN. Oral submucous fibrosis: A new concept in surgical management. Int J Oral Maxillofac Surg 1995;24:433–439.
Turesky S, Gilmore ND, Glickman I, et al. Reduced plaque formation by the chloromethyl analogue of vitamin C. J Periodontol 1970;41(1):41–43. DOI: 10.1902/jop.1922.214.171.124.
Loe H, Silness J. Periodontal disease in pregnancy: Prevalence and severity. Acta Odontol Scand 1963;21(6):533–551. DOI: 10.3109/00016356309011240.
Singh NP, McCoy MT, Tice RR, et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175(1):184–191. DOI: 10.1016/0014-4827(88)90265-0.
IARC. Betel-quid and areca-nut chewing. Monographs on the evaluation of the carcinogenic risk of chemicals to humans. Lyon, France: International Agency for Research on Cancer; 1985;37:141–202.
Waerhaug J. Prevalence of periodontal disease in Ceylon. Association with age, sex, oral hygiene, socioeconomic factors, vitamin deficiencies, malnutrition, betel and tobacco consumption and ethnic group final report. Acta Odontol Scand 1967;25(2):205–231. DOI: 10.3109/00016356709028749.
Jeng JH, Lan WH, Hahn LJ, et al. Inhibition of the migration, attachment, spreading, growth and collagen synthesis of human gingival fibroblasts by arecoline a major alkaloid, in vitro. J Oral Pathol Med 1996;25(7):371–375. DOI: 10.1111/j.1600-0714.1996.tb00281.x.
Papa S, Skulachev VP. Reactive oxygen species, mitochondria, apoptosis and aging. Mol Cell Biochem 1997;174(1-2):305–319. DOI: 10.1023/A:1006873518427.
Sundqvist NJ, Bartschh K, Grafstromr C, et al. Cytotoxic and genotoxic effects of areca-nut related compounds in cultured human buccal epithelial cells. Cancer Res 1989;;49(19):5294–5298.
Akhter R, Hassan NM, Aida J, et al. Relationship between betel quid additives and established periodontitis among Bangladeshi subjects. J Clin Periodontol 2008;35(1):9–15. DOI: 10.1111/j.1600-051X.2007.01164.x.
Ling LJ, Hung SL, Tseng SC, et al. Association between betel quid chewing, periodontal status and periodontal pathogens. Oral Microbiol Immunol 2001;16(6):364–369. DOI: 10.1034/j.1399-302x.2001.160608.x.
Reichart PA, Schmidtberg W, Scheifele C. Betel chewer's mucosa in elderly Cambodian women. J Oral Pathol Med. 1996;25(7):367–370. DOI: 10.1111/j.1600-0714.1996.tb00280.x.
Wyk CW, Olivier A, Miranda CM, et al. Observations on the effect of areca nut extracts onoral fibroblast proliferation. J Oral Pathol Med 1994;23(4):145–148. DOI: 10.1111/j.1600-0714.1994.tb01103.x.
Fisher MA, Taylor GW, Tilashalski KR. Smokeless tobacco and severe active periodontal disease, NHANES III. J Dent Res 2005;84(8):705–710. DOI: 10.1177/154405910508400804.
Martinez CP, Lorca A, Magan R. Smoking and periodontal disease severity. J Clin Periodontol 1995;22(10):743–749. DOI: 10.1111/j.1600-051X.1995.tb00256.x.
Axell TE. Oral mucosal changes related to smokeless tobacco usage: research findings in Scandinavia. Eur J Cancer B Oral Oncol 1993;29B(4):299–302. DOI: 10.1016/0964-1955(93)90052-g.
Poulson TC, Lindenmuth JE, Greer RO. A comparison of the use of smokeless tobacco in rural and urban teenagers. CA Cancer J Clin 1984;34(5):248–261. DOI: 10.3322/canjclin.34.5.248.
Augustine D, Rao RS, Anbu J, et al. In vitro cytotoxic and apoptotic induction effect of earthworm coelomic fluid of Eudrilus eugeniae, Eisenia foetida, and Perionyx excavatus on human oral squamous cell carcinoma-9 cell line. Toxicol Rep 2019;6:347–357. DOI: 10.1016/j.toxrep.2019.04.005.